Deli Bal ve Grayanotoksin’in Karaciğer Dokusu Üzerindeki Etkilerinin Zamana Bağlı Araştırılması

Year 2020, , 97 - 111, 02.03.2020

Hümeyra Haksoy Gülgün Çakmak-arslan , Pınar Göç-rasgele , Meral Kekeçoğlu

https://doi.org/10.18521/ktd.598739

Cited By: 1

Abstract

Amaç: İçindeki grayanotoksin
bileşiğinden dolayı insanlarda zehirlenmeye sebep olan deli bal (DB), özellikle
Türkiye’nin Karadeniz bölgesinde bazı rahatsızlıkların tedavisinde yaygın
olarak kullanılmaktadır. DB zehirlenmesindeki semptomların 1-2 gün içinde
normale döndüğü rapor edilmiş olmasına rağmen, iyileşme süresi hakkında
ayrıntılı bir çalışma mevcut değildir. Zehirlenme etkilerinin ne kadar süreli
olduğunun bilinmesi, hastalara doğru tedavi metodunun uygulanması ve zamanında
taburcu edilebilmeleri açısından önemlidir. Bu çalışmanın amacı, etken doz DB
ve içindeki aktif maddenin saf hali olan GTX’in karaciğer dokusu üzerindeki
toksik etkilerinin zamana bağlı olarak incelenmesidir.

Yöntem:
Mus musculus türü erkek farelere 75
mg/kg (etken doz) DB ve 0,01 mg/kg GTX-III uygulanmış, bu farelerin 24 ve 48
saat sonra karaciğerleri çıkartılmış ve Azaltılmış Toplam Yansıma-Fourier
Dönüşüm Kızılötesi (ATR-FTIR) spektroskopisi ile moleküler düzeyde
incelenmiştir.

Bulgular: 24 saatlik DB
ve GTX uygulaması, karaciğerde lipit peroksidasyonuna, glikojen miktarında
azalmaya, doymuş lipit miktarında artışa, membran düzeninde azalmaya, membran
akışkanlığında artışa, proteinlerin ve nükleik asitlerin yapı ve
konformasyonlarında değişikliklere sebep olmuştur. 48 saatlik süre sonunda glikojen
miktarındaki artış her iki grupta, doymuş lipit miktarındaki artış ise sadece
DB grubunda normale dönmüştür. Fakat, lipit peroksidasyonu, protein ve nükleik
asitlerin yapı ve konformasyonlarındaki, membran düzen ve akışkanlığındaki
değişiklikler için 48 saatlik süre yeterli olmamıştır. 

Sonuç: Sonuç olarak,
24 saatlik DB ve GTX uygulaması karaciğer dokusu üzerinde önemli toksik etkiler
oluşturmaktadır ve bu toksik etkilerin hepsinin normale dönmesi için 48 saatlik
süre yeterli değildir. Çalışmamızın sonuçları, DB’nin etki süresini ve DB
zehirlenmesi vakalarında hastaların tedavi ve taburcu sürelerini belirlemek
için faydalı olacaktır.

Keywords

Deli Bal, FTIR Spektroskopisi, Karaciğer, Grayanotoksin

Supporting Institution

Bu çalışma herhangi bir kurumdan destek almamıştır.

Time-dependent Investigation of Mad Honey and Grayanotoxin on Liver Tissue

Abstract

OBJECTIVE:
Mad Honey (MH), which causes poisoning in people because of grayanotoxin (GTX)
in it, is widely used in the treatment of some disorders, especially in the
Black Sea Region of Turkey. Although it has been reported that symptoms of MH
poisoning return to normal within 1-2 days, there is no detailed study about
the recovery time. It is important to know how long the effects of poisoning
last in order to apply the correct treatment method and to be discharged from
hospital on time. The aim of this study is to investigate the toxic effects of
effective dose of MH and GTX, which is the pure form of the active agent of MH,
on liver tissue at molecular level depending on the time.

METHODS:
Mus musculus male mice were treated
with 75 mg/kg DB (effective dose) and 0.01 mg/kg GTX-III, liver tissues of
these mice were removed after 24 and 48 hours and examined by Attenuated Total
Reflection-Fourier Transformation Infrared (ATR-FTIR) Spectroscopy at molecular
level.

RESULTS:
24 h MH and GTX administration caused lipid peroxidation, a decrease in
glycogen amount, an increase in saturated lipid amount, a decrease in membrane
order, an increase in membrane fluidity, changes in the structure and
conformations of proteins and nucleic acids. After 48 h the decrease in
glycogen amount returned to normal values in both groups, the increase in the
saturated lipids returned to normal values only in the MH group. However, the
48 h-period were not sufficient to return to normal values for the lipid
peroxidation, changes in the structure and conformation of proteins and nucleic
acids and membrane order and fluidity.

CONCLUSION:
In conclusion, 24 hour MH and GTX treatment induces toxic effects on liver
tissue and the 48 h-period is not sufficient for normalization of all these
toxic effects. The results of this study will be useful to determine the
duration of treatment and discharge from hospital for patients with MH
poisoning.

Keywords

Mad Honey, FTIR Spectroscopy, Liver, Grayanotoxin

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