Yerli Kara Düvelerde Süperovulasyon ve Embriyo Verimi Üzerine Farklı FSH Uygulamalarının Etkileri

Year 2016, Volume: 9 Issue: 4, 322 - 326, 01.12.2016

Umut Taşdemir , Tahir Karaşahin Muharrem Satılmış Sedat Hamzi Kızıl Mustafa Kaymaz

Abstract

Bu çalışmanın amacı Yerli Kara düvelerde süperovulasyon oluşturma ve embriyo elde etme yönünde iki farklı follikül uyarıcı hormonunun etkinliğini gözlemlemekti. Düvelere 1.56 g progesteron içeren Cue-mate uygulandı. Düveler dört gruba ayrıldı. Folltropin (F) grubuna control olarak 400 mg folltropin diğer taraftan Ovagen (O) grubuna ise sırasıyla 8.8 mg (O1), 11.4 (O2) ve 17.6 (O3) olmak üzere Ovagen uygulandı. Süperovulasyon protokolünün 9. gününde Cue-mate çıkarıldı ve gruplardaki tüm ineklere 500 μg prostaglandinF2α yapıldı. 11. gün akşam ve 12. gün sabah olmak üzere 12 saat ara ile tüm ineklere Yerli Kara boğalardan alınan spermalar ile suni tohumlama yapıldı. Suni tohumlama uygulamasından 7 gün sonra embriyo elde etme çalışması ile embriyolar toplandı. Ortalama CL sayıları F grubunda 6.33 ± 0.718 belirlenirken, O grubunda uygulanan üç farklı dozdan (sırasıyla 3.82 ± 0.502, 3.50 ± 0.513, 3.58 ± 0.448; p<0.001) elde edilen CL sayılarından daha yüksek bulundu. Transfer edilebilir embriyo oranları uygulama grupları arasında farklı değildi (p>0.05). Sonuç olarak, bulgular F grubunda yapılan uygulamanın CL, elde edilen embriyo ve ovum sayısını artırmasına rağmen transfer edilebilir embriyo sayısına olumlu bir etkisinin olmadığını gösterdi.

Keywords

Yerli Kara Düve, Folltropin, FSH, Ovagen, Süperovulasyon

Various FSH Administration on Superovulatory Response and Embryo Yield in Anatolian Black Heifers

Abstract

The aim of the present study was to investigate effectiveness of superovulatory response and embryo yield in Anatolian Black heifers, induced with the administration of two different follicle stimulating hormone. Heifers received a progesterone releasing device (Cue-mate containing 1.56 g progesterone). The heifers were randomly assigned to four groups. Group Folltropin (F) were administered with Folltropin (400 mg) as a control, while those included in Groups Ovagen (O) were administered with Ovagen at doses of 8.8 mg (O1), 11.44 mg (O2) and 17.6 mg (O3) respectively. On the 9th day of the application, the Cue-mate was removed and the heifers received 500 μg prostaglandinF2α in all goups. The heifers were artificially inseminated (AI) using semen obtained from Anatolian Black bulls; in the evening of day 11 and in the morning of day 12 with 12 hours intervals. Embryos were collected by uterine flushing 7 days after AI. The mean number of CL determined as 6.33±0.718 in F, was found to be higher than the numbers obtained with the administration of the three different doses of O (3.82±0.502, 3.50±0.513, 3.58±0.448 respectively; p<0.001). The transferable embryo yield did not differ significantly among the treatment groups (p>0.05). In conclusion, findings show that although the administration of F was ascertained increase the number of CL and the total number of ova/embryos recovered, these increased numbers had no reflection on the number of transferable embryos in Anatolian Black heifers.

Keywords

Anatolian Black heifer, Folltropin, FSH, Ovagen, Superovulation

References

• Ağaoğlu AR, Kaymaz M, Ağaoğlu OK, Karakaş K, Yağcı İP, Taşdemir U. Effect of different gonadotropin preparation on ovulatory response and embryo yield in Karayaka Ewes. Kafkas Univ. Vet. Fak. Derg. 2012; 18: 861-864.
• Barati F, Niasari-Naslaji A, Bolourchi M, Sarhaddi F, Razavi K, Naghzali E, Thatcher WW. Superovulatory response of Sistani cattle to three different doses of FSH during winter and summer. Theriogenology. 2006; 66: 1149-1155.
• Baruselli PS, Sa Filho MF, Martins CM, Nasser LF, Nogueria MFG, Barros CM, Bo GA. Superovulation and embryo transfer in Bos indicus cattle. Theriogenology. 2006; 65: 77-88.
• Carballo GD, Tribulo A, Tribulo R, Tríbulo H, Bo GA. Superovulation in the first follicular wave without the use of estradiol in bonsmara cattle. Reprod. Fertil. Dev. 2008; 20: 226.
• Carvalho BC, Varago FC, Ruas JRM, Vargas MW, Santos GB, Silva AM. Embryo production in zebu cows after superovulation with two commercial formulations of gonadotropin. R. Bras. Ci. Vet. 2013; 20: 155-159.
• Compan D, Cividini A. Editors. Brachycerous cattle in Balkan countries and their perspectives. ERFP Press, Domzale, 2008; pp. 44-46.
• Gonzalez A, Lussier JG, Camtthers TD, Murphy BD, Mapletoft RJ. Superovulation of beef heifers with Folltropin: a new preparation containing reduced LH activity. Theriogenology. 1990; 33: 519-529.
• Hasler JF. Current status and potential of embryo transfer and reproductive technology in dairy cattle. J. Dairy Sci. 1992; 75: 2857-2879.
• Herrler A, Elsaesser F, Parvizi N, Niemann H. Superovulation of dairy cows with purified FSH supplemented with defined amounts of LH. Theriogenology. 1991; 35: 633-643.
• Hockley DK, Bo GA, Palasz AT, Del Campo MR, Mapletoft RJ. Superovulation with a single subcutaneous injection of Folltropin in cow: Effect of dose and site of injection. Theriogenology. 1992; 37: 224.
• Ireland JJ, Ward F, Jimenez-Krassel F, Ireland JL, Smith GW, Lonergan P, Evans AC. Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle. Human Reprod. 2007; 22: 1687-1695.
• Kafi M, McGowan MR. Factors associated with variation in the superovulatory response of cattle. Anim. Reprod. Sci. 1997; 48: 137-157.
• Kanagawa H, Shimohira I, Saitoh N. Manual of Bovine Embryo Transfer. Japan Livestock Technology Association, Tokyo, 1995; pp. 285
• Kelly P, Duffy P, Roche JF, Boland MP. Superovulation in cattle: effect of FSH type and method of administration on follicular growth, ovulatory response and endocrine patterns. Anim. Reprod. Sci. 1997; 46: 1-14.
• Kim IH, Son DS, Yeon SH, Choi SW, Park SB, Ryu IS. Effect of dominant follicle removal before superstimulation on follicular growth, ovulation and embryo production in Holstein cows. Theriogenology. 2001; 55: 937–945.
• Mapletoft RJ, Steward KB, Adams GP. Recent advances in the superovulation in cattle. Reprod. Nutr. Dev. 2002; 42: 601-611.
• McNatty KP, Hudson NL, Ball K, Mason A, Simmons MH. Super-ovulation and embryo recovery in goats treated with Ovagen and Folltropin. New Z. Vet. J. 1989; 37: 27-29.
• Nilchuen P, Chomchai S, Rattanatabtimtong S. Superovulation with different doses of follicle stimulating hormone in Kamphaeng Saen beef cattle. J. Anim. Vet. Adv. 2012; 11 (5): 676-680.
• Quaresma MA, Costa LL, Silva JR. Superovulation of Mertolenga cows with two FSH preparations (FSH-P and FOLLTROPIN). Rev. Port. Ci. Vet. 2003; 98: 81-84.
• Silva JCC, Alvarez RH, Zanenga CA, Pereira GT. Factors affecting embryo production in superovulated Nelore cattle. Anim. Reprod. 2009; 6: 440-445.
• Solti L, Crichton EG, Loskutoff NM, Cseh S. Economical and ecological importance of indigenous livestock and application of assisted reproduction to their preservation. Theriogenology. 2000; 53: 149-162.
• Stringfellow DA, Seidel GE. Editors. Photographic illustration of embryo developmental stage and quality codes. 3. Ed. Savoy, Manuel of International Embryo Transfer Society, IL, IETS. 1998.
• Taşdemir U, Ağaoğlu AR, Kaymaz M, Karakaş K. Ovarian response and embryo yield of Angora and Kilis goats given the Day 0 Protocol for superovulation in the non- breeding season. Trop. Anim. Health Prod. 2011; 43: 1035-1038.
• Taşdemir U, Satılmış M, Karaşahin T, Kızıl SH, Kaymaz M, Imai K. The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow. Ank. Univ. Vet. Fak. Derg. 2012; 59: 211-216.
• Wildt DE, Monfort ST, Donoghue AM, Johnston LA, Howard J. Embryogenesis in conservative biology or, how to make an endangered species embryo. Theriogenology. 1992; 37: 161-184