Farklı Ekilibrasyon Ortamlarının Çözüm Sonu Boğa Sperması Kalitesi Üzerine Etkisi

Year 2017, Volume: 10 Issue: 2, 57 - 62, 01.03.2017

Mehmet Borga Tırpan Kemal Tuna Olğaç , Hakan Gürler Ufuk Kaya

Abstract

Ekilibrasyon, spermanın sıcaklığının düşürüldüğü ve faz değişimlerinin meydana geldiği aşama olarak, sperma dondurulmasında en önemli basamaklardan biridir. Ekilibrasyonun gerçekleştirildiği ortam çoğu zaman göz ardı edilmekte, ekilibrasyon koşullarının başarısı ampirik tecrübelere dayanmaktadır. Bu çalışmanın amacı farklı ekilibrasyon ortamlarının toplam motilite (TMOT), progresif motilite (PMOT), spermatozoa hareket parametleri (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plazma membran ve akrozom bütünlüğü (PMAI) ve DNA fragmentasyon indeksi (DFI) üzerine etkisinin araştırılmasıdır. Çalışmada 4 baş boğadan iki kez sperma alınarak üç eşit hacme bölündü. Andromed® ile sulandırılan spermalar 24 saat 4°C’de payet içinde (Deneme 1), kapta (Deneme 2) ve çalkalayıcıda (Deneme 3) ekilibrasyona bırakıldı. Çözüm sonu sperma kalitesi TMOT, PMOT, VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF, PMAI ve DFI parametreleri değerlendirilerek belirlendi. Farklı ekilibrasyon koşulları arasında, hareket özellikleri olan TMOT, PMOT, VSL, VAP, BCF ve morfolojik özellikler olan PMAI ve DFI açısından istatistiki bir farklılık bulunamadı (p>0,05). En yüksek VCL değeri Deneme 3’te, en yüksek LIN, STR, WOB ve ALH değerleri Deneme 2’de gözlendi (p<0,05). Bu sonuçlar farklı kolullarda yapılan ekilibrasyonun çözüm sonu spermatolojik değerler üzerinde herhangi bir zararlı etkisi olmadığını göstermektedir. Ayrıca boğa spermasının çalkalayıcıda ekilibre edilmesinin kap ve payet içinde ekilibre edilmesine göre spermatozoa hareket özellikleri açısından daha yararlı olduğu söylenebilir.

Keywords

Boğa Sperması, CASA, Ekilibrasyon, Eklibrasyon Ortamı, Flow Sitometri

Effects of Different Equilibration Conditions on Cryopreserved Bovine Sperm Quality

Abstract

Equilibration is the one of the important steps of semen cryopreservation and it is the stage in which semen is cooled and phase changes occur. The condition of equilibration is mostly regarded; and success of the equilibration conditions depend on empirical experiences. The aim of this study was to investigate the effects of three different equilibration methods on post-thaw total motility (TMOT), progressive motility (PMOT), kinetic parameters of spermatozoa (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plasma membrane and acrosome integrity (PMAI) and DNA fragmentation index (DFI). In each of 4 bulls; two ejaculates were split into three aliquots, diluted by Andromed® and equilibrated for 24h at 4°C in Drawed Straw (Experiment 1), Cups (Experiment 2) and Shaker (Experiment 3). After thawing, sperm quality was determined by examination of TMOT, PMOT, VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF, PMAI and DFI. There were no differences (p>0.05) depending on equilibration conditions for TMOT, PMOT, VSL, VAP, BCF as kinetic parameters and for PMAI and DFI as morphological parameters. The highest VCL value was observed in experiment 3, when the highest LIN, STR, WOB and ALH values were observed in experiment 2 (p<0.05). The results show that changes in equilibrating condition have no detrimental effect on post-thaw bull semen quality. In addition, it can be said that equilibrating bull semen in a shaker presents some kinetic benefits in contrast with cup or straw equilibration methods.

Keywords

Bull Semen, CASA, Equilibration, Equilibration Condition, Flow Cytometry

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