Ratlarda Renal İskemi/Reperfüzyon Hasarında Curcumin'in Etkileri

Year 2018, Volume: 11 Issue: 3, 215 - 222, 01.09.2018

Zülfükar Sarıtaş , Hazen Sarıtaş Musa Korkmaz , M.fatih Bozkurt , Aziz Bülbül Rengin Elsürer Afşar Tuba Berra Sarıtaş

https://doi.org/10.30607/kvj.416649

Cited By: 1

Abstract

Bu
araştırmada, Renal İskemi/Reperfüzyon Hasarı Modelinde Curcumin'in etkilerini
biyokimyasal ve histopatolojik olarak araştırmak amaçlanmıştır. Çalışmada
ratlar kontrol (C), sham, iskemi repürfüzyon (I/R) ve curcumin (CUR)  grubu olmak üzere (n=6) toplam 24 dişi Wistar
Rat kullanılmıştır. Genel anestezi altında Grup I/R ve CUR da laparatomi
uygulanarak sol böbrek pedikülü diseke edilmiş ve böbrek arteri klempe edilerek
1 saat iskemi ve 6 saat reperfüzyon gerçekleştirilmiştir. Grup CUR da 500 mg/kg
Curcumin iskemi sonrası intraperitonal olarak verilmiştir. Serum MPO, IMA, MDA,
NO, SOD, GPx, AOS , üre ve kreatinin ölçümleri gerçekleştirilmiştir. Doku
örneklerinde MPO, MDA, NO, SOD, GPx, AOS ölçülmüştür. Histopatolojik inceleme
ile böbrek dokusunda I/R hasarı skorlanmıştır. Serum ve doku AOS Grup I/R Grup
CUR ile karşılaştırıldığında istatistiksel önemi olacak şekilde Grup CUR'de
yüksel bulunmuştur (p<0.05). Doku NO düzeyi I/R grubu ile karşılaştırıldığında
Grup CUR’da istatistiksel önemi olacak şekilde düşük bulunmuştur (15,30±5,41,
4,8±1,37) (p<0.05). Histopatolojik skorlamada Grup CUR, Grup I/R ile
karşılaştırıldığında istatistiksel olarak anlamlı olacak şekilde düşük
bulunmuştur (p<0.05). Sonuç olarak; bu araştırmada Curcumin’in Renal I/R
hasarı modelinde serum ve doku örneklerinde oksidatif stresi azaltarak I/R
hasarını önlemede olumlu etkisi olduğu ve histopatolojik inceleme ile de
Curcumin'in bu olumlu etkisi desteklenmiştir.

Keywords

Curcumin, Renal İskemi/Reperfüzyon, Rat.

Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats

Abstract

This study aimed to
investigate the biochemical and histopathological effects of curcumin on Renal
Ischemia/Reperfusion Injury. Twenty-four female Wistar rats were used in the
study and divided into control (C), sham, ischemia/reperfusion (I/R), and curcimin
(CUR) groups (n=6). Laparotomy was
performed under general anesthesia in I/R and CUR groups than the left renal
pedicle was dissected it was clamped. Then, 1-h ischemia and 6-h reperfusion
were applied. 500 mg/kg Curcumin was given intraperitoneally to the CUR group
after ischemia application. MPO, IMA,
MDA, NO, SOD, GPx, AOS, urea, and creatinine levels were measured in serum
samples. MPO, MDA, NO, SOD, GPx, and AOS were also measured in tissue samples.
The histopathological examination was performed. Serum and tissue AOS levels
were significantly higher in the CUR group than in the I/R group (P<0.05). Tissue NO levels were
significantly lower in the CUR group than in the I/R group (15.30±5.41 and 4.8±1.37,
respectively) (P<0.05).
Histopathological scores were also significantly lower in the CUR group than in
the I/R group (P<0.05). The
results showed that curcumin prevented I/R damage by decreasing oxidative
stress in serum and tissue samples in rat renal I/R model. 

Keywords

Curcumin, Renal Ischemia/Reperfusion, Rats

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