Glifosat Bazlı Herbisite Maruz Kalan Sıçanlarda N-Asetilsisteinin Testis Oksidatif Hasarı, Spermatolojik Parametreler ve DNA Hasarı Üzerindeki Koruyucu Etkisi

Year 2018, Volume: 11 Issue: 3, 292 - 300, 01.09.2018

Fatih Avdatek , Ruhi Türkmen , Hasan Hüseyin Demirel Yavuz Osman Birdane

https://doi.org/10.30607/kvj.435112

Cited By: 1

Abstract

Bu çalışmanın amacı,
N-asetilsisteinin (NAC) sıçanlarda Glifosat (GLF) 'nin neden olduğu testiküler
oksidatif hasar, spermatolojik parametreler ve DNA hasarı üzerindeki koruyucu
etkisini incelemektir. Toplam yirmi sekiz Wistar erkek sıçan, eşit bir şekilde
dört gruba ayrılarak değerlendirildi. Kontrol grubunu olan grup I'deki
sıçanlar, GLF veya NAC olmaksızın normal diyetle beslendiler, grup II’deki
hayvanlara 160 mg / kg / günlük NAC içeren normal rat diyeti uygulandı, grup
III’deki hayvanlara 375 mg / kg / günlük GLF içeren normal rat diyeti verildi
ve grup IV’deki hayvanlara 160 mg / kg / günlük NAC + 375 mg / kg / günlük GLF
içeren normal rat diyeti uygulandı. GLF uygulaması sıçanların testis dokusunda
spermatozoon motilitesini, anormal spermatozoon oranını, spermatozoon plazma
membran bütünlüğünü, glutatyon ve süperoksit dismutaz düzeyini azaltmıştır.
Diğer taraftan GLF uygulanan grupta yüksek malondialdehid düzeyi ve DNA hasarı
saptandı. Ayrıca histopatolojik olarak testis dokusunda spermatozoon
konsantrasyonunda azalma ve sertoli hücrelerin dejenerasyonu belirlendi. NAC ve
NAC + GLF uygulaması, lipid peroksidasyonunu ve GLF tarafından indüklenen DNA
hasarını, sıçanların testislerinde antioksidan enzimlerin aktivitesini ve hücre
bütünlüğünü tersine çevirdi. Yukarıda belirtilen bulgular, NAC'nin GLF'nin
neden olduğu lipit peroksidasyonunu azalttığını, antioksidan savunma
mekanizmasını geliştirdiğini ve sıçanların testislerinde doku hasarını rejenere
ettiğini göstermektedir.

Keywords

N-asetilsistesin, Glifosat, Rat spermatozoon, DNA hasarı

Protective Effect of N-Acetylcysteine on Testicular Oxidative Damage, Spermatological Parameters and DNA Damage in Glyphosate-Based Herbicide-Exposed Rats

Abstract

The aim of this
study was to examine the protective effect of N-acetylcysteine (NAC) on
testicular oxidative damage, spermatological parameters and DNA damage caused
by Glyphosate (GLF) in rats. In
total, twenty-eight Wistar male
rats were evaluated by being separated into four groups in an equal way. Rats
in group I, which represented the control
group, were fed normal diet without GLF
or NAC, group II received normal feed containing
160 mg/kg/daily NAC, group III received normal feed containing 375 mg/kg/daily
GLF, and group IV received normal feed containing 160 mg/kg/daily NAC + 375
mg/kg/daily GLF. GLF administration decreased sperm motility, abnormal
sperm rate, sperm plasma membrane integrity,
glutathione level and superoxide dismutase in the rats’ testicular tissue. On
the other hand, high malondialdehyde level and DNA damage were detected in the
group administered with GLF. Besides, in histopathological terms, a decrease in
sperm concentration and degeneration of sertoli cells were determined in the
testicular tissue. NAC and NAC+GLF administration reversed lipid peroxidation
and DNA damage induced by GLF, the activity
of antioxidant enzymes and cell integrity in rats’ testis. The above-mentioned
findings indicate that NAC reduces lipid peroxidation caused by GLF, improves
the antioxidant defense mechanism and regenerates tissue damage in rats’
testis.

Keywords

N-acetylcysteine, Glyphosate, Rat sperm, DNA damage

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