Giardia duodenalis ile Doğal Enfekte Kuzularda Serum 25 (OH) D3 Seviyeleri

Year 2019, Volume: 12 Issue: 1, 71 - 74, 31.03.2019

Güzin Camkerten , Hasan Erdoğan , Deniz Alıç Ural , İlker Camkerten , Songül Erdoğan , Kerem Ural

https://doi.org/10.30607/kvj.492805

Cited By: 2

Abstract

Giardia duodenalis kuşlar, reptiller ve
bir çok memeli hayvanı enfekte edebilen binükleuslu flagellalı bir
protozoondur. Söz konusu patojen insanlarda ve çiftlik hayvanlarında başta
olmak üzere şekillenen ishalin en önemli nedenleri arasında yer almaktadır.
Çalışmada Giardia duodenalis ile
doğal enfekte kuzularda serum 25-hidroksi vitamin D3 seviyelerindeki
değişimlerin belirlenmesi amaçlanmıştır. Araştırmanın hayvan materyalini Aydın
bölgesinde bulunan yaşları 15-35 günlük arasında her iki cinsiyetten sakız ırkı
Giardia duodenalis ile doğal enfekte
(n=30), benzer yaş aralığındaki aynı ırktan (n=15) ve her iki cinsiyetten
sağlıklı kuzular oluşturdu. Enfekte ve sağlıklı hayvanlardan dışkı örnekleri
rektal tuşe yöntemi ile steril dışkı örnekleri, serum 25-hidroksi vitamin D3
seviyelerinin belirlenmesi amacı ile de tekniğine uygun olarak v.jugularisden
toplamda 5 ml olacak şekilde kan örnekleri alındı. Dışkı örneklerinden direkt
mikroskobik bakı yöntemi ile giardia taraması yapıldı. Elde edilen serum
örneklerinden Savant marka flöresan immunoassay cihazı ile 25-OH-D seviyeleri
ölçüldü. İstatistiksel analizde Mann-Whitney U testinden yararlanıldı. Sağlıklı
ve doğal enfekte hayvanlara ait 25-hidroksi vitamin D3 seviyeleri arasında
istatistiksel (p<0,05) anlamlı değişimlerin olduğu belirlendi.

Keywords

Giardiasis, kuzu, 25-hidroksi vitamin D3

Levels of Serum 25 (OH) D3 in Naturally Infected Lambs With Giardia duodenalis

Abstract

Giardia duodenalis is a binuclear flagellated protozoan that can infect birds, reptiles and many mammals. The pathogen is one of the most important causes of diarrhea in humans and farm animals. The aim of this study was to determine the changes in serum 25-hydroxyvitamin D3 levels in naturally infected lambs with Giardia duodenalis. The animal material of the study was used Sakız sheep which had naturally infected with Giardia duodenalis between 15-35 days of age from the Aydın region (n = 30), healthy lambs in the same age range (n = 15) and both sexes. Sterile stool samples with the method of rectal touching of fecal samples from infected and healthy animals, blood samples were obtained in a total of 5 ml of v.jugularis according to the technique in order to determine serum 25-hydroxyvitamin D3 levels. Giardia screening was performed by direct microscopic examination of stool samples. 25-OH-D levels were measured with Savant brand fluorescent immunoassay device from the obtained serum samples. Mann-Whitney U test was used in statistical analysis. Statistically significant changes were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p <0.05).

Keywords

Giardiasis, Lamb, 25-hydroxy vitamin D3

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