Brusella melitensis Şuşlarının İlk İzolasyonu İçin Farrell Besiyerine Eritritol İlavesinin Değerlendirilmesi

Year 2019, Volume: 12 Issue: 1, 75 - 81, 31.03.2019

Mustafa Sencer Karagül

https://doi.org/10.30607/kvj.494891

Cited By: 3

Abstract

Bu çalışmanın amacı, B.melitensis şuşlarının ilk izolasyonu için selektif Farrel
besiyerine (FB) eritritol ilavesini değerlendirmektir. Selektif besiyerleri ve
özellikle FB, Brusella (B.)
etkenlerinin izolasyonunda yaygın olarak kullanılmaktadır. Bruselloz teşhisinde
altın standart bakteri izolasyonudur. Selektif besiyerlerinin izolasyon
başarısı içeriklerine bağlıdır. Özellikle kontamine örneklerde izolasyon yüzdesi
düşebilmektedir. Bu bakımdan Brusella
türlerinin besiyerlerinde üremelerini provoke edecek eritritol gibi
bileşenlerin selektif besiyerlerine ilavesi izolasyona fayda sağlayabilir.
Çalışmada Brusella melitensis
şuşlarından kaynaklı 14 küçük ruminant atık vakasına ait iç organ numuneleri
kullanılmıştır. Örneklerden hazırlanan suspansiyonlar eş zamanlı olarak FB ve
eritritol ilaveli Farrel besiyerine (F+ER) inokule edilmiştir. İnkubasyon
boyunca besiyerleri gözlemlenmiş ve besiyerlerinin gösterdiği izolasyon ve
inhibisyon performansları takip edilmiştir. Özellikle eritriol ilavesi, koloni
gelişim hızı ve koloni çapı büyüklüğü sağlayarak daha erken izolasyon imkanı
verebilmesi açısından karşılaştırılmıştır. 14 numune arasında 12’si için F+ER
besiyerinde koloni gelişim hızı veya koloni çapı büyüklüğüne göre izolasyon
için avantaj elde edilmiştir. Sonuçlar selektif besiyerine eritritol ilavesinin
tavsiye edilmesini özellikle kontaminant inhibisyonun yetersiz kaldığı
numuneler için desteklemektedir. Bu nedenle çalışmada olduğu gibi eritritolun,
FB gibi selektif besiyeri formulasyonlarına dahil edilmesi ile yüksek izolasyon
başarısının elde edilmesi mümkün görünmektedir.  

Keywords

Brusella melitensis, eritritol, Farrell, izolasyon, selektif besiyeri

Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella melitensis Strains

Abstract

This study aims to evaluate the addition of erythritol
to Farrell medium (FM) for the primary isolation of B.melitensis strains. Selective media, particularly FM, is commonly
used for Brucella isolation.
Isolation is the gold standard for the diagnosis of brucellosis. The isolation
success of selective media depends on their ingredients. Isolation rate may
decrease particularly in contaminated samples; therefore, the addition of
components like erythritol that augments the growth of Brucella species on media can support isolation. In this study,
organ samples associated with 14 small ruminant abortion cases caused by B.melitensis strains were utilized.
Organ suspensions were inoculated to FM and Farrel medium with erythritol
(F+ER) simultaneously. The media’s isolation and inhibition performance was
observed during incubation. The addition of erythritol was evaluated based on
whether it provides earlier isolation with the help of increasing the colony
growth rate and colony size. According to the findings, F+ER is more
advantageous for 12 samples among 14 through isolation as it enables faster
colony growth and larger colonies. The results confirm the recommendation of
adding erythritol particularly in the samples with inadequate contaminant
inhibition. Therefore, high isolation performance can be achieved through
erythritol addition to the selective media like FM.

Keywords

Brucella melitensis, erythritol, Farrell, isolation, selective medium

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