Detection of Mycoplasma bovis Infection in Cattle Mammary Tissue by Immunofluorescence and qRT-PCR Methods

Year 2019, Volume: 12 Issue: 2, 110 - 115, 30.06.2019

Serdar Altun , Selçuk Özdemir

https://doi.org/10.30607/kvj.493259

Abstract

Mastitis in cattle
causes important economic losses in all over the world and Mycoplasma bovis is one of the important agent among pathogens
shown to be responsible for this disease. The aim of this study was to
determine the presence and
prevalence of Mycoplasma bovis among
the pathological agents implicated in mastitis by immunofluorescence staining
and qRT-PCR methods in cattle mammary tissue. For this aim, 120 mammary samples
with or without macroscopic lesions as this agent causes subclinical mastitis,
were collected. Mastitis was
diagnosed in 78 of 120 cases. Chronic mastitis cases in 56 and, acute mastitis
in 22 cases were histopathologically diagnosed. 
Fluorescent positivity was determined 28/120 according to the results of
immunofluorescence staining for all sections. Immunpositive signs of Mycoplasma bovis were commonly observed
in samples with chronic mastitis. These results were confirmed by qRT-PCR. According
to these results the presence of Mycoplasma
bovis agent that an important threat to herd health is 23,3%.  

Keywords

Cattle, Subclinical Mastitis, Mycoplasma bovis, qRT-PCR, Immunoflourescence

Sığır Meme Dokularında Mycoplasma bovis Enfeksiyonunun İmmunofloresan ve qRT-PCR Yöntemleriyle Araştırılması

Abstract

Sığırlarda Mastitis
tüm dünyada önemli ekonomik kayıplara neden olmaktadır.  Mycoplasma
bovis ise bu hastalıktan sorumlu olarak gösterilen patojenler arasında en
önemli bakteriyel etkenlerden biridir. Bu çalışmanın amacı, sığır meme
dokusunda oluşan mastitisde rol oynayan patolojik ajanlar arasında Mycoplasma
bovis'in varlığı ve prevalansını immunofloresan boyama ve qRT-PCR yöntemleriyle
ve saptamaktır.  Bu amaçla, etkenin
subklinik mastitise yol açtığı düşünülerek, makroskobik olarak lezyonlu ya da
lezyonsuz olunmasına bakılmaksızın 120 adet meme örneği toplandı. Toplanan 120
örneğin 56 kronik ve 22 akut olmak üzere toplam 78 örnekte histopatolojik
olarak mastitis tanısı kondu.  Tüm
kesitlere uygulanan immünofloresan boyama sonuçlarına göre floresan pozitifliği
28/120 olarak belirlendi. Mycoplasma
bovis immünopozitiflikleri daha yoğun olarak kronik mastitisli örneklerde
gözlendi. Bu sonuçlar örnklere uygulanan qRT-PCR sonuçları ile uyumludur. Bu
sonuçlara göre, Erzurum'da sürü sağlığı için önemli bir tehdit olan Mycoplasma bovis etken varlığı %23,3
oranında tespit edilmiştir. 

Keywords

Sığır, Subklinik mastitis, Mycoplasma bovis, qRT-PCR, İmmunfloresa

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