Bazı Deniz Alg Türlerinin Etanol ve Metanol Ekstraktlarının Antibakteriyel Aktivitelerinin Yersinia ruckeri Üzerinde Araştırılması

Year 2019, Volume: 12 Issue: 3, 226 - 234, 30.09.2019

Jale Korun , Emine Şükran Okudan , Remziye Eda Yardımcı Gülşen Timur , Aycan Ulutaş Mehmet Gökoğlu , Yağmur Saadet Çelik , Beytullah Ahmet Balcı

https://doi.org/10.30607/kvj.522959

Cited By: 1

Abstract

Bu çalışmada Liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta), Padina pavonica (Ocrophyta) ve Sargassum vulgare (Ocrophyta) alglerinin
etanol ve metanol ekstraktlarının hasta gökkuşağı alabalığı (Oncorhynhus mykiss)’ndan izole edilen Yersinia ruckeri suşları üzerine
antibakteriyel aktiviteleri değerlendirilmiştir. Ayrıca, suşların çeşitli
antibiyotiklere duyarlılıkları da çalışılmıştır. Balıklardan izole edilen
suşlar fenotipik testler, API 20E hızlı tanı kiti ve PZR sonuçlarına göre Yersinia ruckeri olarak tanımlanmıştır.
Çalışmada L. ceranoides, H. scoparia, P. pavonica ve S. vulgare
makroalglerinin metanol ekstraktlarının Y.
ruckeri’ye karşı antibakteriyel etki
göstermediği bulunmuştur. P. pavonica’nın etanol ekstraktı Y. ruckeri’ye
karşı düşük antibakteriyel aktivite göstermiştir. L. ceranoides, H. scoparia
ve S. vulgare makroalglerinin etanol ekstraktlarının bakteriye karşı
antibakteriyel aktivite göstermediği tespit edilmiştir. Antibiyogram test
sonuçlarına göre suşların ampisilin, flumekuin, oksitetrasiklin ve trimetoprime
duyarlı oldukları bulunmuştur. Suşlar eritromisine karşı orta derecede direnç
göstermiştir. Sonuç olarak, alglerin antibakteriyel aktiviteye sahip
olduklarının bildirilmesine karşın her alg türünün antibakteriyel aktivite
gösteremeyeceği anlaşılmıştır. 

Keywords

Gökkuşağı alabalığı, Yersinia ruckeri, deniz algleri, antibakteriyel aktivite

Investigation of Antibacterial Activities of Ethanol and Methanol Extracts of Some Marine Algae Species on Yersinia ruckeri

Abstract

In this study,
antibacterial activities of extracts of Liagora ceranoides (Rhodophyta), Halopteris
scoparia (Ocrophyta), Padina pavonica (Ocrophyta) and Sargassum
vulgare (Ocrophyta) on Yersinia
ruckeri strains isolated from sick rainbow trout (Oncorhynhus mykiss) were evaluated. Also, susceptibilities of the
strains against various antibiotics were studied. The strains isolated from the
sick fish were identified as Yersinia
ruckeri according to the results of the phenotypic tests, the API 20E rapid
diagnostic kit and PCR. In the study, it was found that the methanol extracts
of L. ceranoides, H. scoparia, P. pavonica and S. vulgare
did not show antibacterial activity against Y. ruckeri. The ethanol
extract of P. pavonica showed low antibacterial activity against Y. ruckeri.
The ethanol extracts of L. ceranoides, H. scoparia and S. vulgare
macroalgae did not show antibacterial activity against the bacterium. According
to the results of the antibiogram test, it was found that the strains were sensitive
against ampicillin, flumequine, oxytetracycline and trimethoprime. The strains
showed intermediate resistance against erythromycine. In conclusion, although
it was reported that algae had antibacterial activities, it was understood that
every algae species could not exhibit antibacterial activity. 

Keywords

Rainbow trout, Yersinia ruckeri, marine algae, antibacterial activity

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