Sezon Dışı Pırlak Koç Sperma Sulandırıcısına İlave Edilen Lipoik Asid ve Trolox’un Dondurma ve Çözdürme Sonrası Spermatolojik Parametreler, Oksidatif Stres ve DNA Hasarı Üzerine

Year 2019, Volume: 12 Issue: 3, 363 - 369, 30.09.2019

Fatih Avdatek , Deniz Yeni , Muhammed Kürşad Birdane , Mustafa Gündoğan

https://doi.org/10.30607/kvj.592883

Cited By: 4

Abstract

Bu çalışmanın amacı sezon dışı dönemde Pırlak koç sperma
sulandırıcısına ilave edilen trolox ve alfa lipoik asitin (ALA) çözüm sonu
spermatolojik parametreler, oksidatif stres ve DNA hasarı üzerine etkileri
araştırıldı. Çalışmada sperma örnekleri 5 sağlıklı Pırlak koçtan (2-3 yaş) elde
edildi ve her koçtan 6 ejakulat toplanarak karıştırıldı. Ejakulatlar ml’de
150x10⁶ spermatozoon olacak şekilde antioksidan içermeyen (kontrol)
ve antioksidan içeren 1 mM ALA ve 1 mM trolox sulandırıcılar ile sulandırıldı.
Sulandırılan örnekler 0,25 ml’lik payetlerde 5 ⁰C’de 3 saat
ekilibrasyona tabi tutularak  sıvı azot
buharında donduruldu,37 ^oC'de çözdürülerek spermatolojik
parametreler, DNA hasarı, oksidatif stres açısından değerlendirildi. ALA
grubunda kontrol grubuna göre spermatozoa motilitesi, akrozom ve DNA
bütünlüğü  istatistiki (p<0.05) olarak daha yüksek bulundu. Trolox
grubunda kontrol grubuna göre daha yüksek akrozom ve membran bütünlüğü ile  daha az DNA hasarı tespit edildi.  (p<0.05) . Kontrol
grubu ile karşılaştırıldığında, ALA ve trolox gruplarındaki oksidatif stres
parametrelerindeki azalmalar istatistikî olarak önemli (P<0.05) bulundu. Bu
çalışmanın sonuçlarına göre üreme mevsimi dışında Pırlak koç sperma
sulandırıcısına ilave edilen ALA ve troloxun çözüm sonu spermatolojik
parametreleri, oksidatif stresi ve DNA hasarına karşı koruyucu bir etki
gösterdiği kanaatine varıldı.

Keywords

Alfa lipoik asit, sezon dışı üreme, koç spermatozoon, trolox

Supporting Institution

Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

17.KARİYER.78

Thanks

Bu çalışma Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 17.KARİYER.78 nolu proje ile desteklenmiştir.

Influence of Trolox and Alpha-Lipoic Acid on Post-Thawed Pırlak Ram Sperm Parameters, Oxidative Stress and DNA Damage in Non-Breeding Season

Abstract

The present study was
conducted to examine the protective roles of trolox and alpha-lipoic on
post-thawed Pirlak ram sperm parameters, oxidative stress and DNA damage in
non-breeding season. Semen samples from 5 healty Pırlak rams (2-3 years of age)
were used in the study. Six ejaculates for each rams were collected and pooled.
Ejaculates were split into three aliquots and diluted to a final concentration
of 150x10⁶ spermatozoa/ml with the base extender containing 1 mM
alpha lipoic acid, 1 mM trolox and no additive (control) were cooled to 5 °C
and equilibrated for 3 h then frozen in 0.25 ml French straws. Samples were
thawed in 37 ^oC and evaluated in terms of spermatological
parameters, DNA damage, oxidative stress. Sperm
motility was increased, acrosome rate and DNA damage were decreased
significantly (p<0.05) in ALA, head
abnormal sperm rate and membrane integrity were increased, acrosome rate and
DNA damege were decreased significantly (P
< 0.05) in trolox when compared to the
control group. Compared with the control group, reductions in spermatozoon MDA
and GSH levels were statistically significant (P <0.05) in lipoic acid and
trolox groups. Results of this study suggest
that alpha-lipoic acid and trolox improve sperm parmeters, oxidative stress and
DNA damage in non-breeding season.

Keywords

Alpha-lipoic acid, Non-breeding season, Ram sperm, trolox

Project Number

17.KARİYER.78

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