Morphometric and Stereological Assessment of The Bovine Vesicular Gland

Year 2020, Volume: 13 Issue: 3, 254 - 261, 30.09.2020

Murat Sirri Akosman , Vural Özdemir

Abstract

The vesicular gland is an important genital gland related to the fertility of bovine. In this study, the morphometric and stereological methods were sought to adapt to the bovine vesicular gland. For this aim, 8 healthy Holstein bull’s paired vesicular glands were collected from the slaughterhouse. The weight and the dimensions of the glands were measured before the fixation. After the formalin fixation, all the glands were divided into 3 subgroups for the total volume estimation of the gland, cell counting and the volume estimation of the cell nucleus. The cell counting and the volume estimation of the cell nucleus were performed on the principal cells. The average weight, width, length, height, the volume of the glands, the mean number and nucleus volume of the principal cells were 30.9 g., 2.62 cm, 8.86 cm and 1.86 cm, 29.7 cm3, 3.58.109 and 108 µm3, respectively. The researchers believe that the findings will contribute to the literature and in particular, facilitate experimental and toxicological researches performed in the future.

Keywords

Cell number, Nucleus volume, Optical fractionator, Stereology, Vesicular gland

Supporting Institution

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Project Number

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Thanks

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Sığır Veziküler Bezinin Morfometrik ve Stereolojik Olarak Değerlendirilmesi

Abstract

Veziküler bez sığır fertilitesinde önemli bir üreme bezidir. Sunulan bu çalışmada sığır veziküler bezinin morfometrik ve stereolojik olarak incelenmesi amaçlanmıştır. Bu amaçla 8 sağlıklı Holstein boğasının çift olan veziküler bezleri mezbahadan toplanmış ve ağırlığı ile boyutları ölçülmüştür. Formaldehit tespitinden sonra tüm bezler bezin toplam hacminin, hücre sayısının ve çekirdek hacminin ölçülmesi için 3 alt gruba ayrılmıştır. Hücre sayımı ve hücre çekirdek hacmi ölçümü bezin en çok bulunan ana hücresi olan principal hücreler üzerinde gerçekleştirilmiştir. Bezin ortalama ağırlığı, eni, uzunluğu, yüksekliği, ortalama hacmi, principal hücrelerin ortalama sayısı ve ortalama çekirdek hacmi sırasıyla 30,9 gr., 2,62 cm, 8,86 cm, 1,86 cm, 29,7 cm3, 3,58,109 ve 108 µm3 olarak bulunmuştur. Araştırmacılar bu bulguların literature katkı sağlayacağına ve gelecekte yapılacak olan deneysel ve toksikolojik çalışmalara ışık tutacağına inanmaktadır.

Project Number

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