Damascus Keçilerinde Süt Yağ Asidi Profili ile SCD, FASN ve SREBPF1 Genlerinin Ekspresyon Seviyeleri Arasındaki İlişki

Year 2020, Volume: 13 Issue: 3, 294 - 303, 30.09.2020

Hüseyin Özkan , Akın Yakan

https://doi.org/10.30607/kvj.728554

Abstract

Bu çalışmada, doymuş yağ asidi içeriği düşük (LSFA) ve yüksek (HSFA) olan keçi sütlerinde SCD, FASN, SREBPF1 genlerinin ekspresyon düzeyleri ile süt yağ asidi profilleri ve aralarındaki ilişki araştırılmıştır. HSFA grubunda süt somatik hücrelerinde SCD, FASN ve SREBPF1 genleri, LSFA grubuna göre yaklaşık sırasıyla 7, 9 ve 4 kat daha fazla ifade edilmiştir (P<0,01). Ayrıca, SCD ve FASN (0,907; P<0,001), SCD ve SREBPF1 (0,628; P<0,001), FASN ve SREBPF1 (0,720; P <0,001) genleri arasında pozitif korelasyonlar tespit edilmiştir. C6:0 (Kaproik asit) ile SCD (0,468; P<0,05) ve SREBPF1 (0,388; P<0,05) genleri arasında pozitif ve önemli korelasyonlar bulunmuştur. Bununla birlikte, bu üç genin tamamı ile C8:0 (Kaprik asit) ve C10:0 (Kaprilik asit) yağ asitleri arasında pozitif korelasyonlar belirlenmiştir. Süt örneklerinde C14:0 (Miristik asit) ve SREBPF1 geni arasında pozitif korelasyon gözlenmiştir (0,469; P<0,05). Ayrıca, koku indeksi ile SCD (0,553; P<0,01), FASN (0,444; P<0,05), SREBPF1 (0,499, P<0,05) genleri arasında pozitif korelasyon tespit edilmiştir. Sonuçlar, SCD, FASN ve SREBPF1 genlerinin keçilerin süt yağ asidi profili ve süt kalitesi üzerinde önemli etkileri olduğunu ve bu genlerin keçilerde seleksiyon uygulamalarında aday olabileceğini göstermiştir.

Keywords

Gen ekspresyonu, Keçi, Süt yağ asitleri, Somatik hücre

The Relationship Between Milk Fatty Acid Profile and Expression Levels of SCD, FASN and SREBPF1 Genes in Damascus Dairy Goats

Abstract

In this study, the relationship between the expression levels of SCD, FASN, SREBPF1 genes and milk fatty acid profiles in goat milks with low (LSFA) and high (HSFA) saturated fatty acid content was investigated and correlated. In HSFA group, SCD, FASN and SREBPF1 genes were approximately 7, 9 and 4 folds more expressed than LSFA in milk somatic cells, respectively (P<0.01). Also, positive correlations were determined between SCD and FASN (0.907; P<0.001), SCD and SREBPF1 (0.628; P<0.001), FASN and SREBPF1 (0.720; P <0.001) genes. Positive and important correlation was found between C6:0 (Caproic acid) and SCD (0.468; P<0.05) and SREBPF1 (0.388; P<0.05) genes. On the other hand, positive correlations were found between all of these three genes and C8:0 (Capric acid) and C10:0 (Caprylic acid). In milk samples, C14:0 (Myristic acid) and SREBPF1 genes were correlated positively (0.469; P<0.05). Moreover, positive correlation was found between the odour index and SCD (0.553; P<0.01), FASN (0.444; P<0.05), SREBPF1 (0.499, P<0.05) genes. The results showed that SCD, FASN and SREBPF1 genes have important effects on the milk fatty acid profile and milk quality of goats and these genes may be candidate in selection applications in goats.

Keywords

Gene expression, Goat, Milk fatty acids, Somatic cells

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