Somatic Cell Count and Bacteriological Evaluation of Milk Obtained from Clinically Healthy Goat

Year 2021, Volume: 14 Issue: 4, 451 - 457, 31.12.2021

Nevzat Saat , Hakan Tavşanlı , Ziya İlhan

https://doi.org/10.30607/kvj.978222

Abstract

In this study, it was aimed to analyze goat milk samples in terms of somatic cell number, isolation of aerobic bacteria, to determine the in vitro susceptibility of isolated S. aureus strains to various antibiotics and to reveal methicillin resistant S. aureus strains. The mean number of somatic cells of 313 milk samples examined was 3.25x103, 1.9x106 for 214 (68.3%) milk samples with subclinical mastitis, and 1x106 cells/mL for 32 (27.6%) milk samples with S. aureus isolated. Bacteria were isolated from 116 (54.2%) milk samples by culture method. Coagulase negative staphylococci from 46 (39.6%) and S. aureus from 32 (27.6%) isolated. Of the 32 S. aureus strains tested, 27 (84.3%) were resistant to penicillin G, 17 (53.1%) to amoxicillin/clavulanic acid and cefaprezone, 15 (46.9%) to marbofloxacin, 13 (34.3%) to enrofloxacin, 9 (28.1%) to ceftiofur and 8 (25.0%) to gentamicin. Eight of the S. aureus strains (25.0%) had methicillin-resistant S. aureus and 5 (62.5%) had multi-antibiotic resistance. As a result, it was determined that i) the mean somatic cell count was low, ii) S. aureus strains were highly resistant to penicillin G, amoxicillin/clavulanic acid and cefaperozone, iii) methicillin-resistant S. aureus was found to be 25% of the rate.

Keywords

Somatic cell count, Isolation, Methicillin resistance Staphylococcus aureus, Goat, Mastitis

Klinik Olarak Sağlıklı Görünen Saanen Keçilerden Alınan Sütlerin Somatik Hücre Sayısı ve Bakteriyolojik Yönden İncelenmesi

Abstract

Bu çalışmada keçi sütü örneklerinin somatik hücre sayısı yönünden analizi, aerobik bakterin izolasyonu, izole edilen S. aureus suşlarının çeşitli antibiyotiklere in vitro duyarlılıklarının saptanması ve metisilin dirençli S. aureus suşlarının ortaya konulması amaçlandı. Toplam 313 süt örneğinin ortalama somatik hücre sayısı 3,25x103, subklinik mastitisli 214 (%68,3) süt örneğinin 1,9x106, S. aureus izole edilen 32 (%27,6) süt örneğinin ise 1x106 hücre/ml olarak belirlendi. Kültür yöntemiyle sütlerin 116 (%54,2) adetinden bakteri izole edildi. Örneklerin 46’sından (%39,6) koagulaz negatif stafilokoklar, 32’sinden S. aureus (%27,6) izole edildi. Toplam 7 farklı antibiyotiğin test edildiği bu çalışmada, 32 adet S. aureus suşundan 27’si (%84,3) penisilin G’ye, 17’si (%53,1) amoksisilin/klavulonik asit ve sefaprezone, 15’i (%46,8) marbofloksasine, 13’ü (%40,6) enrofloksasine, 9’u (%28,1) seftiofura ve 8’i (%25) ise gentamisine dirençli bulundu. S. aureus suşlarından 8’inin (%25,0) metisilin dirençli S. aureus ve 5’i (%62,5), çoklu antibiyotik dirence sahipti. Sonuç olarak, i) ortalama somatik hücre sayısının düşük olduğu ii) S. aureus suşlarının penisilin G, amoksisilin/klavulonik asit ve sefaperozone yüksek düzeyde dirençli olduğu, iii) metisilin dirençli S. aureus oranının %25 olduğu görüldü.

Keywords

Keçi, Mastitis, Somatik hücre sayısı, İzolasyon, Metisilin dirençli Staphylococcus aures

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