Cryopreservation of Ram Semen Using Capsaicin Supplemented Tris Extender

Year 2023, Volume: 16 Issue: 2, 166 - 173, 30.06.2023

Muhammed Enes İnanç , Fatih Avdatek , Şükrü Güngör , Mehmet Fuat Gülhan , Kemal Tuna Olğaç , Barış Denk , Deniz Yeni , Umut Taşdemir

https://doi.org/10.30607/kvj.1257228

Cited By: 1

Abstract

In this study, it was designed to reveal the effects of capsaicin on oxidative stress and freezability of ram semen. Ejaculates were taken from Sönmez rams and divided into five specimens and diluted with extender at different rates (4 mM, 2 mM, 1 mM, 500 µM) with and without capsaicin (control; C). Semen samples were thawed with a 37°C water bath for 30 seconds for post-thawed analysis. At the end of the study, sperm motility and kinetic parameters, plasma membrane acrosome integrity (PMAI), mitochondrial membrane potential (MMP), DNA damage, oxidant and antioxidant parameters were analyzed. A decrease was observed in the groups containing capsaicin compared to the C in terms of progressive, total motility and kinetic parameters (p<0.05). Besides, positive results were not obtained DNA integrity, PMAI and MMP (p<0.05). In conclusion; it was determined that capsaicin added to Tris extender did not have a positive effect on oxidative stress and freezing of ram semen.

Keywords

Spermatozoa, capsaicin, ram, antioxidant

Supporting Institution

Aksaray University Scientific Research Fund

Project Number

BAP2021/008

Thanks

The authors would like to thank Kapucuoğlu sheep farm for the use of their facilities and herd.

Kapsaisin Katkılı Tris Sulandırıcı Kullanılarak Koç Spermasının Dondurularak Saklanması

Abstract

Bu çalışmada, kapsaisinin oksidatif stres ve koç spermasının dondurulabilirliği üzerine etkilerinin ortaya konması tasarlandı. Ejakülatlar Sönmez ırkı koçlardan alınarak beş eşit kısma ayrıldı ve farklı oranlarda (4 mM, 2 mM, 1 mM, 500 µM) kapsaisin içeren ve içermeyen (kontrol) sulandırıcı ile sulandırıldı. Sperma örnekleri çözüm sonu spermatolojik analizler için 37°C sıcaklıklta 30 saniye süre ile çözdürüldü. Çalışma sonunda spermatozoa hareketliliği ve kinetik parametreleri, plazma membran akrozom bütünlüğü (PMAI), mitokondrial membran potansiyeli (MMP), DNA hasarı, oksidan ve antioksidan parametreler analiz edildi. Progresif, total motilite ve kinetik parametreler bakımından kontrol grubuna göre kapsaisin içeren guruplarda azalma görüldü (p<0.05). Ayrıca DNA bütünlüğü, PMAI ve MMP'de olumlu sonuçlar elde edilmedi (p<0.05). Sonuç olarak; Tris sulandırıcısına ilave edilen kapsaisinin, oksidatif stres ve koç spermasının dondurulması üzerine olumlu bir etki göstermediği belirlendi.

Keywords

Spermatozaa, kapsaisin, koç, antioksidan

Project Number

BAP2021/008

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