The evaluation of fertility achieved by modified different ovsynch treatments in holstein cows

Year 2023, Volume: 16 Issue: 3, 444 - 449, 30.09.2023

Muhammed Fahri Kalkan Murat Selcuk

https://doi.org/10.30607/kvj.1344638

Abstract

The aim of this study was to evaluate the effect of modified preovsynch and ovsynch synchronization methods on fertility in dairy cows. A total of 60 Holstein dairy cows were used in the study. Dairy cows were divided into two groups as Group I (n = 30) modified preovsynch, Group II (n = 30) modified ovsynch. In the study, the amount of cervical mucus was recorded from the last GnRH injection until artificial insemination for monitoring of oestrus. Pregnancy examinations were performed by ultrasonography on the 40th day after artificial insemination. At the end of the study, pregnancy rates obtained from modified preovsynch and modified ovsynch were 63.33±0.08% and 56.66±0.09%, respectively. Cervical mucus in 16 dairy cows of modified presynch was light whereas 14 pregnant cows of modified preovsynch had normal cervical mucus. A total of 23 dairy cows in the modified ovsynch had light cervical mucus, while 4 dairy cows had normal cervical mucus. Furthermore, while cervical mucus was intensive in one dairy cow, two dairy cows did not have cervical mucus. In conclusion, the pregnancy results obtained from in the modified preovsynch and ovsynch protocols were similar to each other, but many studies conducted in herds with high number of dairy cows are required to determine the effectiveness of the modified protocols.

Keywords

Dairy cow, preovsynch, ovsynch, fertility

Holştayn İneklerde Modifiye Farklı Ovsynch Uygulamaları İle Elde Edilen Dölveriminin Değerlendirilmesi

Abstract

Yapılan bu çalışmanın amacı süt ineklerinde modifiye preovsynch ve ovsynch senkronizasyon yöntemlerinin dölverimi üzerine etkisinin değerlendirilmesidir. Çalışmada Holştayn ırkı toplam 60 baş süt ineği kullanılmıştır. Süt inekleri I. Grup (n=30) modifiye preovsynch, II. Grup (n=30) modifiye ovsynch olmak üzere iki gruba ayrılmıştır. Çalışmada östrus takibi için son GnRH enjeksiyonundan suni tohumlama yapılana kadarki sürede çara miktarı kaydedildi. Suni tohumlama sonrası 40. günde ultrasonografi ile gebelik muayeneleri yapıldı. Araştırma sonunda modifiye preovsynch ve modifiye ovsynchten elde edilen gebelik oranları sırasıyla %63.33±0.08 ve 56.66±0.09 olarak tespit edildi (p>0.05). Modifiye preovsynch grubunda bulunan süt ineklerinin 16 başında hafif, 14 başında normal çara görülmüştür. Modifiye ovsynch grubunda bulunan süt ineklerinin 23 başında hafif, 4 başında normal, 1 başında yoğun çara tespit edilmiş ve 2 başında ise çara görülmemiştir. Sonuç olarak, preovsynch protokolünde elde edilen gebelik sonuçlarının ovsynch protokolünden elde edilen sonuçlarla birbirine benzer olduğu ancak modifiye protokollerin etkinliğinin belirlenmesi için daha fazla sayıda süt ineğine sahip sürülerde yapılacak çalışmalara gereksinim olduğu düşünülmektedir.

Keywords

Süt ineği, preovsynch, ovsynch, dölverimi

References

• Adataş, T. (2006). İneklerde Ovsynch Ve Co-Synch Yöntemleri İle Ovulasyonun Senkronizasyonu. . A. Ü. Sağlık Bilimleri Enstitüsü Ankara, Yüksek Lisans Tezi
• Alkan KK & Alkan H. (2020). Holstein ırkı ineklerde target breeding ve presynch-ovsynch senkronizasyonyöntemlerinin postpartum ilk tohumlamada gebe kalma oranı üzerine etkisi. Eurasian J Vet Sci. 36 (1): 36-41.
• Ax R, Pollard B, McCauley T, Fish D & Faber S. (2005). Hormone options to increase pregnancy rates. 108th Annual Meeting, Minnesota Veterinary Medical Association, 04 February 2005, Minnesota, USA
• Bartolome JA, Silvestre FT, Kamimura S, Arteche ACM, Melendez P, Kelbert D, McHale J, Swift K, Archbald LF & Thatcher WW (2005). Resynchronization of ovulation and timed insemination in lactating dairy cows I: Use of the Ovsynch and Heatsynch protocols after non-pregnancy diagnosis by ultrasonography. Theriogenology. 63: 1617-1627.
• Bleach, E. C. L., R. G. Glencross, & P. G. Knight. (2004) Associa-tion between ovarian follicle development and pregnancy rates indairy cows undergoing spontaneous oestrous cycles. Reproduction. 127:621-629.
• Burke, J.M., R.L. De La Sota, Rısco, C.A., Staple, C.R., Schmıtt, E.J.P.&Thatcher, W.W. (1996). Evaluation of time insemination using agonadotropin-releasing hormone agonist in lactating dairy cows. J Dairy Sci. 79:1385-1393.
• Carvalho, P. D., J. N. Guenther, M. J. Fuenzalida, M. C. Amundson, M. C. Wiltbank, & P. M. Fricke. (2014). Presynchronization using a modified Ovsynch protocol or a single gonadotropin-releasing hormone injection 7 d before an Ovsynch-56 protocol for submission of lactating dairy cows to first timed artificial insemination. J. Dairy Sci. 97:6305-6315.
• Carvalho PD, Fuenzalida MJ, Ricci AH, Souza RV, Barletta MC, Wiltbank MC, &Fricke PM. (2015a) Modifications to Ovsynch improve fertility during resynchronization: Evaluation of presynchronization with GnRH 6 days before Ovsynch and addition of a second prostaglandin F2α treatment. J Dairy Sci, 98: 8741–8752
• Carvalho PD, Wiltbank MC & Fricke PM. (2015b) Manipulation of progesterone to increase ovulatory response to the first GnRH treatment of an Ovsynch protocol in lactating dairy cows receiving first timed artificial insemination. J Dairy Sci, 98: 8800-8813
• Dinç, D.A. (2006). İneklerde Reprodüktif Verimliliği Artırma Programları. Vet Hek Dern Derg. 77, 50-64
• Elibol E., Uçar M. & Yılmaz O., (2009). Ovsynch uygulanan ineklerde sun’i tohumlama sonrası 12. günde yapılan GnRH enjeksiyonunun gebelik oranına etkisi. Kocatepe Veteriner Dergisi. 2, 13- 18.
• Galvão KN & Santos JE. (2010). Factors affecting synchronization and conception rate after the Ovsynch protocol in lactating Holstein cows. Reprod Domest Anim. 45: 439-446.
• Herlihy M.M., Giordano J.O., Souza A.H., Ayres H., Ferreira R M., Keskin A., Nascimento A.B., Guenther J.N., Gaska J. M., Kacuba S.J., Crowe M.A., Butler S.T. & Wiltbank M.C. (2012). Presynchronization with Double- Ovsynch improves fertility at first postpartum artificial insemination in lactating dairy cows. J Dairy Sci. 95(12), 7003-7014.
• Jaśkowski JM, Olechnowicz J & Nowak W. (2006). Some causes of declining fertility in dairy cows. Med Weter. 62: 385-389
• Jaśkowski JM, Herudzinska M, Kulus J, Brüssow KP & Gehrke M. (2018). OvSynch program, its modifications and alternative hormonal programs in cow reproduction. Med Weter. 74: 626-633
• Kara U., Ayaşan T., Hizli H. & Gök K., (2011). Ovsynch protokolünün inek ve düvelerin gebelik oranı üzerine etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 8, 1-8
• Macmillan K.L. (2010). Recent advances in the synchronization of estrus and ovulation in dairy cows. J Reprod Dev. 56: 42-47.
• Martinez M.F., Adams G.P., Kastelic J.P., Bergfelt D.R. & Mapletoft R.J. (2000). Induction of follicular wave emergence for estrus synchronization and artificial insemination in heifers. Theriogenology. 54: 757-769.
• Marquezini G.H., Dahlen C.R., Bird S.L. & Lamb G.C. (2011). Administration of human chorionic gonadotropin to suckled beef cows before ovulation synchronization and fixed-time insemination: replacement of gonadotropin-releasing hormone with human chorionic gonadotropin. J Anim Sci. 89: 3030-3039
• Nak Y., Nak D., Seyrek İntaş K., Tek HB., Keskin A. & Tuna B. (2005). Ovsynch, PRD + PGF2α + PMSG ve norgestomet içeren kulak implantı + PGF2α PMSG ile sağıtılan siklik ve asiklik sütçü ineklerde kızgınlık ve gebelik oranlarının karşılaştırılması. Uludag Universitesi Veteriner Fakültesi Dergisi, 24, 33-39.
• Navanukraw C., Redmer D.A., Reynolds L.P., Kirsch J.D., Grazul-Bilska A.T. & Fricke P.M. (2004). A modified presynchronization protocol improves fertility to timed artificial insemination in lactating dairy cows. J Dairy Sci. 87(5), 1551-1557.
• Nowicki A, Barański W, Baryczka A & Janowski T. (2017). OvSynch protocol and its modifications in the reproduction management of dairy cattle herds. J Vet Res. 61: 329-336
• Pekçok D. & Aksu E.H. (2015). Sığırlarda Östrus Senkronizasyonu ile Birlikte Kullanılan Döl Tutma Oranını Etkileyen Faktörler. Atatürk Üniversitesi Vet. Bil. Derg. 10(3): 205-210.
• Peters MW & Pursley JR (2002). Fertility of lactating dairy cows treated with ovsynch after presynchronization injections of PGF2 and GnRH. J Dairy Sci. 85, 2403-2406.
• Pursley, J.R., Mee, M.O. & Wıltbank (1995). M.C. Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology. 44: 915.
• SAS. (2009). SAS Stat Sofware. SAS Campus Drive Cary, NC. 27513, USA.
• Souza A.H., Ayres H., Ferreira R.M. & Wiltbank M.C. (2008): A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI lactating dairy cows. Theriogenology. 70, 208-215, 2008.
• Wankhade, P.R., Manimaran, A., Kumaresan, A., Jeyakumar, S., Ramesha, K.P., Sejian, V., Rajendran, D. & Varghese, M.R. (2017). Metabolic and immunological changes transition dairy cows: A review. Vet World. 10: 1367-1377
• Whittier, J.C. & Geary, T.W. (2000). Frequently asked questions about synchronizing estrus and ovulation in beef cattle with GnRH. Prepared for Iowa Cooperative Extension CHIPS Beef Breeding Management Seminar, 29 January 2000 Ames, Iowa, USA
• Yılmazbaş-Mecitoğlu G, Karakaya E., Keskin A., Alkan A. & Gumen A. (2013). Reducing the duration between gonadotropin-releasing hormone (GnRH) and prostaglandin F2α treatment in the ovsynch protocol to 6 days improved ovulation to second GnRH treatment, but inclined to reduce fertility. Journal of Dairy Science. 96, 3817-3824.