Aksaray İli Buzağı İshallerinde Escherichia coli ve Escherichia coli Bakterisinin Son Üç Aylık Antibiyogram Sonuçları

Year 2023, Volume: 16 Issue: 4, 606 - 613, 31.12.2023

Ali Evren Haydardedeoğlu , Melek Aydemir , Elif Selin Şenoğlu , Zeki Aras

https://doi.org/10.30607/kvj.1333868

Abstract

Escherichia coli, Enterobacteriaceae ailesinde yer alan gram negatif, fakültatif anaerob, hareketli, spor oluşturmayan çomak şeklinde bir bakteridir. Patojenik E. coli ekstraintestinal ve intestinal olmak üzere iki gruba ayrılır. İntestinal Escherichia coli patotipleri: enterotoksijenik E. coli (ETEC), enteropatojenik E. coli (EPEC), Vero- veya Shiga-toksin üreten E. coli (VTEC veya STEC), enterohemorajik E. coli (EHEC), enteroagregatif E. coli (EAEC), enteroinvaziv E. coli (EIEC) ve diffuz aderent E. coli (DAEC)’dir. Ekstraintestinal patojenik E. coli ise; septisemik patojenik E. coli, üropatojenik E. coli, avian patojenik E. coli, meme patojenik E. coli ve uterus enfeksiyonlarına neden olanlar, endometriyal patojenik E. coli olarak sıralabilir. Enterotoksijenik Escherichia coli (ETEC), buzağılarda neonatal ishalin en yaygın nedenidir ve ince bağırsakta kolonize olan, enterotoksin üreten, neonatal dönemde buzağılar arasında ortaya çıkan bakteriyel bir hastalıktır. Bu çalışmanın amacı 2021 yılının ocak, şubat ve mart ayları arasında, Aksaray bölgesinde bulunan neonatal buzağı ishaline sebep olan E.coli izolatlarını belirlemektir. Çalışmada kullanılmak üzere, Aksaray bölgesindeki 20 farklı sığır işletmesinde ishal kaynaklı ölen neonatal dönemdeki buzağıların iç organ örnekleri (karaciğer, kalp, akciğer ve mezenterik lenf düğümü) alınmıştır. Herbir işletmede ölen bir buzağı örneklemeye dahil edilerek, toplam 20 adet buzağıdan örnek alınmıştır. Bu üç ayı kapsayan iç organ örneklerinin 12'sinde (%60) E.coli izole edilmiştir. Örneklerin antibiyogram sonuçlarına göre izolatların %50'sinin Amoksisilin ve Eritromisine, %33,3'ünün Tetrasikline, %58,3'ün Trimetoprim-sülfametoksazole, %66,6'sının Streptomisine, %75'inin Flofenikol, Gentamisin ve Enrofloksasine, %83,3'ünün Sefloksasin ve Sefloksasinyuma duyarlı olduğu tespit edildi.

Keywords

Neonatal Buzağı, İshal, E.coli, Antibiyogram

Antibiogram Results of Escherichia coli in Calf Diarrhea and Escherichia coli Bacteria in Aksaray Province in The Last Three Months

Abstract

Escherichia coli is a gram-negative, facultative anaerobic, motile, non-spore-forming rod-shaped bacterium belonging to the Enterobacteriaceae family. Pathogenic E. coli are divided into two groups: extraintestinal and intestinal. Intestinal Escherichia coli pathotypes: enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), Vero- or Shiga-toxin-producing E. coli (VTEC or STEC), enterohemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC) and diffusely adherent E. coli (DAEC). Extraintestinal pathogenic E. coli: These can be listed as septicemic pathogenic E. coli, uropathogenic E. coli, avian pathogenic E. coli, breast pathogenic E. coli and those that cause uterine infections, endometrial pathogenic E. coli. Enterotoxigenic Escherichia coli (ETEC) is the most common cause of neonatal diarrhea in calves and is a bacterial disease that colonizes the small intestine, produces enterotoxin, and occurs among calves during the neonatal period. The aim of this study is to determine the E.coli isolates that cause neonatal calf diarrhea in the Aksaray region between January, February and March 2021. To be used in the study, internal organ samples (liver, heart, lung and mesenteric lymph node) of neonatal calves that died due to diarrhea were taken from 20 different cattle farms in the Aksaray region. One calf that died in each farm was included in the sampling, and a total of 20 calves were sampled. E.coli was isolated in 12 (60%) of the internal organ samples covering these three months. According to the antibiogram results of the samples, 50% of the isolates were converted to Amoxicillin and Erythromycin, 33.3% to Tetracycline, 58.3% to Trimethoprim-sulfamethoxazole, 66.6% to Streptomycin, 75% to Flofenicol, Gentamicin and Enrofloxacin, % 83.3 of them were found to be sensitive to Cefloxacin and Cefloxacinium.

Keywords

Neonatal Calf, Diarrhea, E.coli, Antibiogram

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