In vitro Embriyo Kültür Medyumlarına İlave Edilen Eritropoietinin Oksidatif Stres ve Embriyo Gelişimi Üzerine Etkisi

Year 2019, Volume: 59 Issue: Ek Sayı, 107 - 112, 04.06.2020

Muharrem Satılmış , Ali Bilgili

Abstract

Çalışmada, in vitro embriyo kültür medyumuna ilave edilen eritropoietinin antioksidan etkinliği incelendi. Sığır ovaryumlarından elde edilen oositlerin in vitro maturasyonu ve fertilizasyonlarını izleyen süreçte eritropoietinin 3 farklı dozu (0,5 μg, 0,25 μg, 0,125 μg /ml) ilave edilerek 3 deneme grubu ve eritropoietin ilave edilmemiş (antioksidansız) kontrol grubu oluşturularak kültür ortamına alınarak bölünen oosit oranları, blastosiste erişim oranları biyokimyasal parametreler değerlendirildi. Morula ve 48. saat bölünen oosit oranları bakımından gruplar arasındaki farklılık önemli iken, diğer özellikler için gruplar arası farklılıkların istatistiksel olarak önemsiz olduğu tespit edildi (p>0,05).

Keywords

Antioksidan, embriyo, eritropoietin, in vitro, oksidatif stres

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