Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA)

Year 2013, Volume: 1 Issue: 2, 56 - 63, 02.02.2014

Sibel Yavru Oya Bulut Orhan Yapıcı Mehmet Kale , Ayhan Ata

Abstract

Bovine Viral Diarrhea (BVD) is a common infection all over the world. It causes important economical losses in cattle breeding. In this study, a total of 46 blood samples were examined taken into tubes with and without EDTA from candidate bulls in Artifical Insemination Centers. Blood serum samples were tested to detect for antibodies against Bovine Viral Diarrhea Virus (BVDV) and leukocyte samples were tested for BVDV antigens by ELISA methods. Eight (17.3%) out of 46 serum samples were found as positive by the means of antibodies against BVDV while 3 (6.5%) out of 46 leukocyte samples were detected as positive for BVD antigens. One (2.1%) of the seropositive bulls was detected as positive for BVD antigen. Two (4.3%) of the seronegative bulls were detected as positive for BVD antigens.

Keywords

BVDV, Bull, ELISA

Suni Tohumlama Merkezlerine Alınma Öncesi Aday Boğalarda Enzyme Linked Immunosorbent Assay (ELISA) ile Bovine Viral Diarrhea Virus (BVDV) Enfeksiyonunun Serolojik ve Virolojik Olarak Araştırılması

Abstract

Bovine Viral Diarrhea (BVD) tüm dünya üzerinde görülen yaygın bir enfeksiyondur. Sığır yetiĢtiriciliğindeki en önemli ekonomik kayıpların nedenidir. Bu çalıĢmada suni tohumlama merkezlerinde kullanılmak üzere seçilen toplam 46 adet aday boğadan EDTA‟lı ve EDTA‟sız tüplere kan örnekleri alınarak incelendi. Kan serum örnekleri BVDV‟ye karĢı oluĢan antikorlar yönünden, lökosit örnekleri ise BVDV antijen varlığı yönünden ELISA

ile test edildi. 46 adet serum örneğinden 8 (%17.3)

adedi BVDV antikorları yönünden seropozitif olarak

tespit edilirken, lökosit örneklerinden 3 adedi (%6.5)

ise BVDV antijen varlığı yönünden pozitif bulundu. 3

adet BVDV antijen pozitif boğanın 2 adedi (%4.3)

seronegatif iken 1 adedi (%2.1) ise seropozitif olarak

tespit edildi.

Keywords

BVDV, Boğa, ELISA

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