Research Article
Van Bölgesindeki Bal Arılarında (Apis mellifera) Bulunan Varroa destructor’un (AKAR: Varroidae) Genetik Karakterizasyonu
Abstract
Türkiye’de arı yetiştiriciliğinin en önemli
arı zararlısı olarak görülen Varroa destructor, bir ektoparazittir. Çalışmanın amacı, Türkiye’nin Van
bölgesindeki bal arılarını (Apis
mellifera) istila eden Varroa
destructor akarının haplotiplerini belirlemektir. Araştırmada,
akarın mitokondriyal Cox1 gen belgesini analiz etmek için PCR+RFLP modifiye
yöntemi kullanılmıştır. Ayrıca örneklerin % 10’u (28) çift yönlü sekans analizi
için özel bir firmaya gönderilmiştir. PCR sonucunda, 570 bp büyüklüğünde
amplifiye DNA elde edilmiştir. RFLP için amplifiye ürüne XhoI ve SacI restriksiyon
enzimleri uygulanmıştır. SacI
restriksiyon enzimi DNA’yı kesmemesine rağmen XhoI restriksiyon enzimi amplifiye DNA’yı 270 ve 300 bp olarak iki
parçaya (bant) kesmiştir. RFLP reaksiyonunun bantları ve mitokondriyal DNA'nın
dizi analizi, tüm akar örneklerinin V.
destructor Kore haplotipi olduğunu ortaya koymuştur. Bu çalışma sonuçları
Van bölgesinde bu akarların kontrolünde yardımcı olmalıdır.
Keywords
References
- Akinwande KL, Badejo MA, Ogbogu SS. 2012. Incidence of the Korean haplotype of Varroa destructor in southwest Nigeria. J Apic Res. 51(4): 369-370. Anderson D, Trueman JWH. 2000. Varroa jacobsoni (Acari: Varroidae) is more than one species. Exp. Appl. Acarol. 24: 165-189. Anderson DL, Fuchs S. 1998. Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. J. Apic. Res. 37(2): 69-78. Aydın L, Güleğen E, Çakmak İ, Girişgin AO. 2007. The Occurrence of Varroa destructor Anderson and Trueman, 2000 on honey bees (Apis mellifera) in Turkey. Turk. J. Vet. Anim. Sci. 31: 189-191. Beaurepaire AL, Truong TA, Fajardo AC, ve ark. 2015. Host Specificity in the Honeybee Parasitic Mite, Varroa spp. in Apis mellifera and Apis cerana. PLoS ONE. 10(8): 1-12. Chemurot M, Akol AM, Masembe C, ve ark. 2016. Factors influencing the prevalence and infestation levels of Varroa destructor in honeybee colonies in two highland agro-ecological zones of Uganda. Exp. Appl. Acarol. 68(4): 497-508. Crane E. 1978. The Varroa mite. Bee World. 59: 164-167. De Guzman LI, Rinderer TE, Stelzer JA. 1999. Occurrence of two genotypes of Varroa jacobsoni Oud. In North America. Apidologie. 30: 31-36. De Jong D, Gonçalves LS. 1981. The Varroa problem in Brazil. Am. Bee. J. 121: 186-189. Delaplane KS. Varroa destructor: revolution in the making. Bee World 2001; 82(4): 157-159. Gajic B, Radulovic Z, Stevanovic J, ve ark. 2013. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. Exp. Appl. Acarol. 61: 97-105. Maggi M, Medici S, Quintana S, ve ark. 2012. Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina. Exp. Appl. Acarol. 56: 309-318. Muñoz I, Garrido-Bailón E, Martín-Hernández R, ve ark. 2008. Genetic profile of Varroa destructor infesting Apis mellifera iberiensis colonies. J. Apic. Res. 47: 310-313. Navajas M, Anderson DL, De Guzman LI, ve ark. 2010. New Asian types of Varroa destructor. a potential new threat for World apiculture Apidologie. 41: 181-193. Rosenkranz P, Aumeier P, Ziegelmann B. 2010. Biology and control of Varroa destructor. J. Invertebr. Pathol. 103: 96-119. Sakai T, Okada I. 1973. The present beekeeping in Japan. Glean Bee Cult. 101: 356-357. Strapazzon R, Carneiro FE, Guerra Jr JCV, ve ark. 2009. Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the State of Santa Catarina, Brazil. Genet. Mol. Res. 8(3): 990-997. Thompson HM, Brown MA, Ball RF, ve ark. 2002. First report of Varroa destructor resistance to pyrethroids in the UK. Apidologie. 33: 357-366. Warrit N, Hagen TAR, Smith DR, ve ark. 2004. Survey of Varroa destructor strains on Apis mellifera in Turkey. J. Apic. Res. 43(4): 190-191. Webster TC, Delaplane KS. 2001. Mites of Honey Bee, İllinois,:Dadant ve Sons Yayıncılık, s. 280.
Year 2017,
Volume: 5 Issue: 2, 78 - 84, 25.12.2017
Abstract
References
- Akinwande KL, Badejo MA, Ogbogu SS. 2012. Incidence of the Korean haplotype of Varroa destructor in southwest Nigeria. J Apic Res. 51(4): 369-370. Anderson D, Trueman JWH. 2000. Varroa jacobsoni (Acari: Varroidae) is more than one species. Exp. Appl. Acarol. 24: 165-189. Anderson DL, Fuchs S. 1998. Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. J. Apic. Res. 37(2): 69-78. Aydın L, Güleğen E, Çakmak İ, Girişgin AO. 2007. The Occurrence of Varroa destructor Anderson and Trueman, 2000 on honey bees (Apis mellifera) in Turkey. Turk. J. Vet. Anim. Sci. 31: 189-191. Beaurepaire AL, Truong TA, Fajardo AC, ve ark. 2015. Host Specificity in the Honeybee Parasitic Mite, Varroa spp. in Apis mellifera and Apis cerana. PLoS ONE. 10(8): 1-12. Chemurot M, Akol AM, Masembe C, ve ark. 2016. Factors influencing the prevalence and infestation levels of Varroa destructor in honeybee colonies in two highland agro-ecological zones of Uganda. Exp. Appl. Acarol. 68(4): 497-508. Crane E. 1978. The Varroa mite. Bee World. 59: 164-167. De Guzman LI, Rinderer TE, Stelzer JA. 1999. Occurrence of two genotypes of Varroa jacobsoni Oud. In North America. Apidologie. 30: 31-36. De Jong D, Gonçalves LS. 1981. The Varroa problem in Brazil. Am. Bee. J. 121: 186-189. Delaplane KS. Varroa destructor: revolution in the making. Bee World 2001; 82(4): 157-159. Gajic B, Radulovic Z, Stevanovic J, ve ark. 2013. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. Exp. Appl. Acarol. 61: 97-105. Maggi M, Medici S, Quintana S, ve ark. 2012. Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina. Exp. Appl. Acarol. 56: 309-318. Muñoz I, Garrido-Bailón E, Martín-Hernández R, ve ark. 2008. Genetic profile of Varroa destructor infesting Apis mellifera iberiensis colonies. J. Apic. Res. 47: 310-313. Navajas M, Anderson DL, De Guzman LI, ve ark. 2010. New Asian types of Varroa destructor. a potential new threat for World apiculture Apidologie. 41: 181-193. Rosenkranz P, Aumeier P, Ziegelmann B. 2010. Biology and control of Varroa destructor. J. Invertebr. Pathol. 103: 96-119. Sakai T, Okada I. 1973. The present beekeeping in Japan. Glean Bee Cult. 101: 356-357. Strapazzon R, Carneiro FE, Guerra Jr JCV, ve ark. 2009. Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the State of Santa Catarina, Brazil. Genet. Mol. Res. 8(3): 990-997. Thompson HM, Brown MA, Ball RF, ve ark. 2002. First report of Varroa destructor resistance to pyrethroids in the UK. Apidologie. 33: 357-366. Warrit N, Hagen TAR, Smith DR, ve ark. 2004. Survey of Varroa destructor strains on Apis mellifera in Turkey. J. Apic. Res. 43(4): 190-191. Webster TC, Delaplane KS. 2001. Mites of Honey Bee, İllinois,:Dadant ve Sons Yayıncılık, s. 280.
There are 1 citations in total.
Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 25, 2017 |
| Submission Date | November 2, 2017 |
| Published in Issue | Year 2017 Volume: 5 Issue: 2 |
