Buzağılarda Respiratuar Sinsityal Virus (BRSV) Enfeksiyonlarında Patolojik ve İmmunohistokimyasal İncelemeler

Year 2017, Volume: 2 Issue: 2, 131 - 138, 29.12.2017

Zafer Özyıldız , Özlem Özmen , Hüseyin Dolu

https://doi.org/10.24880/maeuvfd.363907

Abstract

Bu çalışmada Burdur İli’ndeki buzağılarda saptanan
respiratuar sinsityal virus (BRSV) enfeksiyonları makroskobik, histopatolojik
ve immunohistokimyasal bulgularıyla detaylı olarak incelendi. Solunum
problemleri ile ölen ve immunohistokimyasal olarak BRSV tespit edilen
buzağıların nekropsisinde makroskobik olarak kraniyo-ventral loblarda koyu
kırmızı renkli sert kıvamlı pnömoni alanları gözlendi. Bu alanların kesit
yüzlerinden köpüklü ve mukopurulent bir eksudat sızıyordu. Mikroskobik olarak akciğer
kesitlerinde intersitisyel ve bronko-intersitisyel pnömoni bulguları gözlendi. Pnömoni
bölgelerinde yoğun şekilde karakteristik sinsityal hücreler ve bazı
bronş-bronşiyol epitellerinde intrasitoplazmik inklüzyon cisimciklerine
rastlandı. İmmunohistokimyasal incelemelerde; bronş, bronşiol ve alveol
epitelleri ile lümenlerindeki yangısal hücre ve özellikle sinsityal hücrelerin sitoplazmalarında
immuno-pozitif alanlara rastlandı. Bu çalışmanın amacı;  Burdur bölgesinde buzağılarda ciddi kayıplara
neden olan BRSV hastalığında makroskobik, mikroskobik ve immunohistokimyasal
bulgularının detaylı olarak sunulmasıdır.

Keywords

BRSV, buzağı, patoloji

Pathological and Immunohistochemical Studies in Respiratory Cyncitial Virus (BRSV) Infection in Calves

Abstract

In this study, bovine respiratory syncytial virus (BRSV) infections of calves were examined
in detail with macroscopic, microscopic and immunohistochemical findings in Burdur province.
At necropsy of calves that died from respiratory findings and immunohistochemically BRSV
diagnosed, dark red and hard areas in the cranio-ventral lobes grossly. Foamy and mucopurulent
exudate was leaking from the cross-sections of these areas. Microscopically, findings of interstitial
and broncho-interstitial pneumonia were observed in the lung sections. At the pneumonic
areas, marked characteristic syncytial cells and intra cytoplasmic inclusion bodies were seen
in some bronchiolar and bronchial epithelial cells. Immunohistochemically, immuno-positive
areas were seen in the bronchial, bronchiolar and alveolar epithelial cells and the cytoplasm of
inflammatory cells especially syncytial cells. The aim of this study was detailed presentation of
macroscopical, microscopical and immunohistochemical findings of BRSV disease in calves
which cause serious economic losses in Burdur province.

Keywords

BRSV, calf, pathology, immunohistochemistry

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