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The Effect of Aluminum Obtained from Natural Conditions on In Vitro Spermatological Parameters at Low Doses in Akkaraman Rams

Year 2020, Volume: 5 Issue: 2, 75 - 78, 31.08.2020
https://doi.org/10.24880/maeuvfd.648962

Abstract

Aluminum is the third most common element in nature and
has expanded its use. It is widely used as a cooking product because it is
cheap, easy to shape, heat resistance and glossy surface structure. Therefore aluminum-based
oven trays, foils and teapots are widely used. Exposure of aluminum to high
temperatures causes micro surface cracking and aluminum migrates to foods. In
this study, 10 grams aluminum foil in 1 liter distilled water was boiled at 180
° C for 2 hours, because of realizing aluminum transition to water in under
normal conditions.
The distilled water
including different aluminum doses were obtained by evaporating of which. The
semen was obtained from 2 Akkarman rams by electro-ejaculator. Rams were housed
in Kirikkale University Veterinary Faculty Facilities.
PBS solution was prepared with distilled water which has
got different amounts of aluminum. The experimental groups were G-1 (control),
G-2 (aluminum water) G-3 (50% concentrated water with aluminum) and G-4 (75% concentrated
water with aluminum ). Semen was added to the groups as 50x106 / ml spermatozoa.
The groups were incubated in a water bath at 37 ° C. All groups were evaluated
for motility, viability and mitochondrial membrane potential at 0, 2, 4, 6
hours.
There was no statistically
significant difference between the groups. In this study, it was determined
that low doses of aluminum had no direct toxic effect on spermatological
parameters in vitro. However, it has been considered that long-term intake of
aluminum at low doses may have toxic effects.




References

  • 1. Alfrey, A. C. (1985). Gastrointestinal absorption of aluminum. Clinical nephrology, 24, S84-7.
  • 2. Domingo, J. L., Gomez, M., Llobet, J. M., Del Castillo, D., & Corbella, J. (1994). Influence of citric, ascorbic and lactic acids on the gastrointestinal absorption of aluminum in uremic rats. Nephron, 66(1), 108-109.
  • 3. Bassioni, G., Mohammed, F. S., Al Zubaidy, E., & Kobrsi, I. (2012). Risk assessment of using aluminum foil in food preparation. Int. J. Electrochem. Sci, 7(5), 4498-4509.
  • 4. Greger, J. L., Goetz, W., & Sullivan, D. (1985). Aluminum levels in foods cooked and stored in aluminum pans, trays and foil. Journal of Food Protection, 48(9), 772-777.
  • 5. WHO (World Health Organization), “Safety evaluation of certain food additives and contaminants”, WHO Food additives Series 46. Joint FAO/WHO Expert Committee on Food Additives (2001).
  • 6. Macrae, R., Robinson, R. K., & Sadler, M. J. (1993). Encyclopaedia of food science, food technology and nutrition.
  • 7. Greger, J. L. (1993). Aluminum metabolism. Annual review of nutrition, 13(1), 43-63.
  • 8. Krasovskiĭ, G. N., Vasukovich, L. Y., & Chariev, O. G. (1979). Experimental study of biological effects of leads and aluminum following oral administration. Environmental health perspectives, 30, 47-51.
  • 9. Domingo, J. L. (1995). Reproductive and developmental toxicity of aluminum: a review. Neurotoxicology and teratology, 17(4), 515-521.
  • 10. Yousef, M. I., Kamel, K. I., El-Guendi, M. I., & El-Demerdash, F. M. (2007). An in vitro study on reproductive toxicity of aluminium chloride on rabbit sperm: the protective role of some antioxidants. Toxicology, 239(3), 213-223.
  • 11. Varisli, O., Agca, C., & Agca, Y. (2015). Influence of extenders and cooling rates on epididymal sperm of Lewis rat strain.
  • 12. Varisli, O., Scott, H., Agca, C., & Agca, Y. (2013). The effects of cooling rates and type of freezing extenders on cryosurvival of rat sperm. Cryobiology, 67(2), 109-116.
  • 13. İçme Suyu Temin Edilen Suların Kalitesi ve Arıtılması Hakkında Yönetmelik. 2019/6/ Temmuz. Resmi Gazete(Sayı:30823) Erişim Adresi: https://www.resmigazete.gov.tr/eskiler/2019/07/20190706-8.htm.
  • 14. World Health Organization. (2003). Atrazine in drinking-water: background document for development of WHO guidelines for drinking-water quality (No. WHO/SDE/WSH/03.04/32). World Health Organization.
  • 15. Ekanem, E. J., et al. (2009). Determination of aluminium in different sources and its contribution to daily dietary intake in Nigeria. Journal of Applied Sciences Research, 5(8), 944-948.
  • 16. Bassioni, G., Mohammed, F. S., Al Zubaidy, E., & Kobrsi, I. (2012). Risk assessment of using aluminum foil in food preparation. Int. J. Electrochem. Sci, 7(5), 4498-4509.
  • 17. Altmann, P., Cunningham, J., Dhanesha, U., Ballard, M., Thompson, J., & Marsh, F. (1999). Disturbance of cerebral function in people exposed to drinking water contaminated with aluminium sulphate: retrospective study of the Camelford water incident. Bmj, 319(7213), 807-811.
  • 18. Ewardson, J. (1992). The Camelford incident. In Second International Conference on Aluminum and Health (pp. 61-64).
  • 19. Zhu, Yanzhu, et al. (2012). Suppressive effects of aluminum trichloride on the T lymphocyte immune function of rats. Food and chemical toxicology, 50(3-4), 532-535.
  • 20. Varisli, O., Taskin, A., & Akyol, N. (2018). Effects of different extenders and additives on liquid storage of Awassi ram semen. Turkish Journal of Veterinary and Animal Sciences, 42(4), 230-242.

Doğal Koşullarda Elde Edilen Alüminyumun Akkaraman Koçlarında Düşük Dozlarda In Vitro Spermatolojik Parametreler Üzerine Etkisi

Year 2020, Volume: 5 Issue: 2, 75 - 78, 31.08.2020
https://doi.org/10.24880/maeuvfd.648962

Abstract




Alüminyum
doğada en çok bulunan üçüncü element olması kullanım alanını
yaygınlaştırmıştır. Ucuz olması, kolay şekil verilebilmesi, ısıya dayanıklılığı
ve parlak yüzey yapısı nedeniyle pişirme ürünü olarak kullanımını yaygınlaşmıştır.
Bu nedenle alüminyum bazlı fırın tepsileri, folyolar ve çaydanlıklar yaygın
olarak kullanılmaktadır. Alüminyumun yüksek ısılara maruz bırakılması yüzeyde
kopmalara ve gıdalara alüminyum geçişine neden olmaktadır. Yapılan çalışmada 10
gram alüminyum folyo 1 litre distile su içerisinde 180 °C de 2 saat süreyle
tutulmuştur. Böylece normal koşullar altında suya alüminyum geçişi
sağlanmıştır. Elde edilen suyun farklı oranlarda buharlaştırılması ile farklı
dozlarda alüminyumlu distile su elde edilmiştir. Elde edilen suyun sperm
üzerine etkisini incelemek için Kırıkkale Üniversitesi Veteriner Fakültesi’ne
ait 2 adet akkaraman koçundan elektro-ejakülatör ile elde edilen sperma
kullanılmıştır. Distile su ve farklı oranlardaki alüminyumlu su ile PBS
solüsyonu hazırlanmıştır. Deney grupları G-1 (kontrol), G-2 (alüminyumlu su)
G-3 (%50 konsantre alüminyumlu su) ve G-4 (%75 konsantre alüminyumlu su) olacak
şekildedir. Sperma 50x106/ml spermatozoa olarak gruplara eklendi.
Gruplar 37 °C de su banyosunda inkubasyona bırakıldı. Tüm gruplar 0, 2, 4, 6. saatlerde
motilite, canlılık ve mitokondriyal membran potansiyeli açısından
değerlendirildi. Gruplar arasındaki fark istatiksel olarak belirlendi ve
gruplar arasında anlamlı bir fark gözlenmedi. 
Bu çalışma ile alüminyumun düşük dozlarda alımının spermatolojik
parametrelere iv vitro olarak doğrudan bir toksik etkisi olmadığı
belirlenmiştir. Fakat alüminyumun düşük dozlarda uzun süreli olarak alınmasının
toksik etkiye sahip olabileceği değerlendirilmektedir.




References

  • 1. Alfrey, A. C. (1985). Gastrointestinal absorption of aluminum. Clinical nephrology, 24, S84-7.
  • 2. Domingo, J. L., Gomez, M., Llobet, J. M., Del Castillo, D., & Corbella, J. (1994). Influence of citric, ascorbic and lactic acids on the gastrointestinal absorption of aluminum in uremic rats. Nephron, 66(1), 108-109.
  • 3. Bassioni, G., Mohammed, F. S., Al Zubaidy, E., & Kobrsi, I. (2012). Risk assessment of using aluminum foil in food preparation. Int. J. Electrochem. Sci, 7(5), 4498-4509.
  • 4. Greger, J. L., Goetz, W., & Sullivan, D. (1985). Aluminum levels in foods cooked and stored in aluminum pans, trays and foil. Journal of Food Protection, 48(9), 772-777.
  • 5. WHO (World Health Organization), “Safety evaluation of certain food additives and contaminants”, WHO Food additives Series 46. Joint FAO/WHO Expert Committee on Food Additives (2001).
  • 6. Macrae, R., Robinson, R. K., & Sadler, M. J. (1993). Encyclopaedia of food science, food technology and nutrition.
  • 7. Greger, J. L. (1993). Aluminum metabolism. Annual review of nutrition, 13(1), 43-63.
  • 8. Krasovskiĭ, G. N., Vasukovich, L. Y., & Chariev, O. G. (1979). Experimental study of biological effects of leads and aluminum following oral administration. Environmental health perspectives, 30, 47-51.
  • 9. Domingo, J. L. (1995). Reproductive and developmental toxicity of aluminum: a review. Neurotoxicology and teratology, 17(4), 515-521.
  • 10. Yousef, M. I., Kamel, K. I., El-Guendi, M. I., & El-Demerdash, F. M. (2007). An in vitro study on reproductive toxicity of aluminium chloride on rabbit sperm: the protective role of some antioxidants. Toxicology, 239(3), 213-223.
  • 11. Varisli, O., Agca, C., & Agca, Y. (2015). Influence of extenders and cooling rates on epididymal sperm of Lewis rat strain.
  • 12. Varisli, O., Scott, H., Agca, C., & Agca, Y. (2013). The effects of cooling rates and type of freezing extenders on cryosurvival of rat sperm. Cryobiology, 67(2), 109-116.
  • 13. İçme Suyu Temin Edilen Suların Kalitesi ve Arıtılması Hakkında Yönetmelik. 2019/6/ Temmuz. Resmi Gazete(Sayı:30823) Erişim Adresi: https://www.resmigazete.gov.tr/eskiler/2019/07/20190706-8.htm.
  • 14. World Health Organization. (2003). Atrazine in drinking-water: background document for development of WHO guidelines for drinking-water quality (No. WHO/SDE/WSH/03.04/32). World Health Organization.
  • 15. Ekanem, E. J., et al. (2009). Determination of aluminium in different sources and its contribution to daily dietary intake in Nigeria. Journal of Applied Sciences Research, 5(8), 944-948.
  • 16. Bassioni, G., Mohammed, F. S., Al Zubaidy, E., & Kobrsi, I. (2012). Risk assessment of using aluminum foil in food preparation. Int. J. Electrochem. Sci, 7(5), 4498-4509.
  • 17. Altmann, P., Cunningham, J., Dhanesha, U., Ballard, M., Thompson, J., & Marsh, F. (1999). Disturbance of cerebral function in people exposed to drinking water contaminated with aluminium sulphate: retrospective study of the Camelford water incident. Bmj, 319(7213), 807-811.
  • 18. Ewardson, J. (1992). The Camelford incident. In Second International Conference on Aluminum and Health (pp. 61-64).
  • 19. Zhu, Yanzhu, et al. (2012). Suppressive effects of aluminum trichloride on the T lymphocyte immune function of rats. Food and chemical toxicology, 50(3-4), 532-535.
  • 20. Varisli, O., Taskin, A., & Akyol, N. (2018). Effects of different extenders and additives on liquid storage of Awassi ram semen. Turkish Journal of Veterinary and Animal Sciences, 42(4), 230-242.
There are 20 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Research Articles
Authors

Abdulkadir Kaya 0000-0001-7903-4358

Ömer Varışlı 0000-0002-2777-3586

Hüsamettin Ekici 0000-0001-6403-737X

Sedat Hamdi Kızıl 0000-0003-0143-1104

Publication Date August 31, 2020
Submission Date November 20, 2019
Published in Issue Year 2020 Volume: 5 Issue: 2

Cite

APA Kaya, A., Varışlı, Ö., Ekici, H., Kızıl, S. H. (2020). Doğal Koşullarda Elde Edilen Alüminyumun Akkaraman Koçlarında Düşük Dozlarda In Vitro Spermatolojik Parametreler Üzerine Etkisi. Veterinary Journal of Mehmet Akif Ersoy University, 5(2), 75-78. https://doi.org/10.24880/maeuvfd.648962