Effects of Entomopathogenic Fungi on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)

Year 2017, Volume: 8 Issue: 2, 191 - 195, 11.09.2017

Alime Bayındır , Ouidad Abdelazız , Ali Kemal Birgücü Amar Oufroukh Mohamed Morad Senoussı İsmail Karaca

Abstract

The study investigated the
efficacy of five entomopathogenic fungi [Aspergillus
sp., Beauveria sp., Fusarium sp., Trichoderma sp. (1) and Trichoderma
sp. (2)] isolated from soil samples which were taken in the wheat farming of INPV (National Institute of
Plant Protection of Constantine, Constantine-Algeria) on the second-stage
larvae of Tuta absoluta (Lepidoptera: Gelechiidae). Spore suspensions of fungi were applied by spraying method after
dilution with sterile distilled water containing 0.02% Tween 80, so that 10⁷
spores/ml concentration. Individuals exposed to distilled water containing
Tween 80 were used as the control group in order to compare the effect of
entomopathogenic fungi. Evaluations of infection development were made at the 1^st,
3^rd, 5^th and 7^th days following applications.
According to the results obtained, the highest mortality at the 7th DAA (Day
After Application) was found as 75.00% and 73.00% from the individuals exposed
to of with Trichoderma sp. (1) and
with Trichoderma sp. (2)
entomopathogenic fungi, respectively. These entomopathogenic fungi followed
%62.00, %45.00 and %41.00 mortality of Aspergillus
sp., Fusarium sp. and Beauveria sp., respectively.

Keywords

Aspergillus sp., Beauveria sp., Fusarium sp., Tuta absoluta, Trichoderma sp.

Entomopatojen Fungusların Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)’ ya Etkileri

Abstract

Bu çalışmada, Ulusal Bitki
Koruma Enstitüsü (INPV-National Institute of Plant Protection of Constantine,
Constantine-Algeria) çevresinden alınan toprak örneklerinden izole edilen 5
farklı entomopatojen fungus [Aspergillus sp.,
Beauveria sp., Fusarium sp., Trichoderma
sp. (1) ve Trichoderma sp. (2)]’un Tuta absoluta Meyrick (Lepidoptera:
Gelechiidae) 2. dönem larvaları üzerine etkinliği araştırılmıştır.
Fungusların spor süspansiyonları 10⁷ spor/ml olacak şekilde %0,02
oranında Tween 80 içeren steril saf su ile seyreltildikten sonra püskürtme
yöntemiyle uygulanırken, Tween 80 içeren steril saf su uygulanmış bireyler ise kontrol
grubu olarak kabul edilmiştir. Enfeksiyon gelişimi değerlendirmeleri,
uygulamalar yapıldıktan 1, 3, 5 ve 7 gün sonra canlı birey sayıları üzerinden
yapılmıştır. Elde edilen sonuçlara göre, uygulamadan sonraki 7. günde, kontrol
grubuna göre en yüksek yüzde ölüm oranı %75,00 ile Trichoderma sp. (1) ve %73,00 ölüm oranı ile Trichoderma sp. (2) entomopatojen funguslarında elde edilmiştir. Bu
fungusları sırasıyla %62,00, %45,00 ve %41,00 ölüm oranı ile - Aspergillus sp., Fusarium sp. ve Beauveria sp.
fungusları takip etmiştir.

Keywords

Trichoderma sp., Aspergillus sp., Fusarium sp., Beauveria sp., Tuta absoluta, Trichoderma sp.

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