Meme Kanseri Hücrelerinde Zamana Bağlı HIF-2α İfadesi Değişiminin Analizi

Year 2017, Volume: 8 Issue: Ek (Suppl.) 1, 245 - 251, 21.11.2017

Muzaffer Dükel , Kevin D. Brown

https://doi.org/10.29048/makufebed.336876

Cited By: 1

Abstract

Meme kanseri
kadınlarda en sık rastlanan kanser türüdür veTürkiye’de gözlemlenen vaka sayısı
her geçen gün artmaktadır. Meme kanserinin içinde yer aldığı birçok kanser
türünde, kanserli doku kan damarlarından uzak bölgelerde büyürken oksijen
eksikliğine maruz kalır. Hipoksiya olarak adlandırılan bu duruma karşı dokular Hipoksi
ile indüklenebilir faktör (HIF) transkripsiyon faktörlerinin ifade artışı ile
cevap verirler. HIF transkripsiyon faktörlerinin etkisiyle kanserli hücreler,
damar gelişimini uyararak farklı doku ve organlara göç ederler. Bu nedenle HIF
transkripsiyon faktörlerinin hipoksiya sürecindeki değişiminin bilinmesi
önemlidir. HIF-2α hipoksik koşullar altında ifadesi artan bir transkripsiyon
faktörüdür ve farklı kanser türlerinde ifade düzeyi değişiklik göstermektedir.
Bu çalışmanın amacı farklı meme kanseri ve normal meme epiteli hücrelerinde
farklı sürelerde hipoksiya uygulamasının HIF-2α ifadesinin değişimi üzerinde
etkisinin araştırılmasıdır.  

Keywords

Meme kanseri, hipoksiya, HIF-2a

Analysis of Time-Dependent Alteration of HIF-2α Expression in Breast Cancer Cells

Abstract

Breast cancer is the
most commonly diagnoised cancer type in women and the number of cases observed
in Turkey has increased day by day. In many types of cancer including breast
cancer, tumor tissues are exposed to oxygen deficiency while growing in areas
distant from blood vessels. Against this condition, knew as hypoxia, tissues respond
via increasing the expression of transcription factors called Hypoxia inducible
factor (HIF). By the effect of HIF transcription factors, cancer cells migrate
to different tissues and organs by stimulating vascular development. Therefore,
it is important to know the change of HIF transcription factors in hypoxia
process. HIF-2α
is a transcription factor, which is over expressed during hypoxic conditions
and have varies the level of expression in different cancer types. The aim of
this study is that to investigate the effect of time dependent hypoxia exposure
on HIF-2α expression in different breast cancer cells and normal
breast epithelial cells. 

Keywords

Breast cancer, hypoxia, HIF-2α

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