Yenilebilen Yabani Mantar Morchella esculenta (L.) Pers.’nın Besinsel Kalitesi ve Biyoaktif Özelliklerinin Değerlendirilmesi

Year 2018, Volume: 9 Issue: 2, 95 - 105, 25.10.2018

Hilal Acay

Abstract

Türkiye zengin ve yenilebilen bir makrofungus çeşitlilğine sahiptir. Bu
çalışma, Mardin’de doğal olarak yetişen Morchella
esculenta (L.) Pers.’nın kimyasal
kompozisyonu, yağ asitleri, aminoasitleri ve biyoaktif özellikleri içeren
parametreleri belirlemeyi amaçlamaktadır. Protein, karbonhidrat, yağ, kül,
diyet lif ve enerji içerikleri sırasıyla 20.64 g/100 g·dw, 75.1g/100 g,
3.25/100 g, 12.4 g/100 g, 30.4 g/100 g ve 4257 kcal/100 g, bulunmuştur.
Linoleik, oleik ve palmitik asit gibi yağ asitleri oldukça fazlaydı. Temel
amino asitler arasında en yüksek valindi ve onu threonin takip etti. 
β-karoten-linoleic asit metoduyla en yüksek total antioksidan aktivite
metanol ekstraktında belirlendi (67.21 mg/ml).
En yüksek DPPH radikalini giderme aktivitesi 89.22 mg/ml ile hekzan ektraktında elde edildi. Diğer taraftan, en
yüksek antimikrobiyal aktivite Pseudomonas
aeruginosa ATCC 27853 ‘a karşı (10 mm)   hekzan ekstraktında elde edildi. Sitotoksik
aktivitenin potansiyelinin konsantrasyon ve eksraktların solvent tipine bağımlı
olduğu bulunmuştur. Etil asetat eksraktı 550 μg/ml  (99.82 %)
konsantrasyon değerinde  (99.82 %)  PC-3 hücre
hattına karşı   IC50 264.33 (μg/ml )
ile önemli inhibisyon etkisi göstermiştir. Sonuçta, günlük besin olarak
kullanılan bu mantar yeni  ilaç
geliştirmek için ve kanser terapisinde  
bir çeşit tedavi kaynağı olabilir, ve ayrıca, M. esculenta organik ekstrakları
biyoaktiviteyi teşvik eden maddeler içerebilir.

Keywords

Morchella esculenta (L.) Pers, kimyasal, bioaktif özellikler, yabani mantarlar

Assessment Of The Nutritional Qualities And Bioactive Properties Of The Wild Edible Mushroom Morchella esculenta (L.) Pers.

Abstract

Turkey has a rich and edible macrofungal diversity. This study aim to determinate parameters that included the chemical composition, fatty acids, amino acids and bioactive  properties of  Morchella esculenta which naturally grown in Mardin. Protein, carbohydrate, fat, ash, dietary fiber and energy contents were found 20.64 g/100 g·dw, 75.1g/100 g, 3.25/100 g, 12.4 g/100 g, 30.4 g/100 g  and 4257 kcal/100 g, respectively. Fatty acids such as linoleic, oleic and palmitic acids were relatively abundant. Among the essential aminoacids valin was the highest amount and this was followed by threonine. The highest total antioxidant activity by β-carotene-linoleic acid metod was identified in methanol extract (67.21 mg /ml). The highest DPPH scavenging activity was obtained hexane extract with 89.22 mg/ml.  On the other hand, the highest antimicrobial activity was obtained against Pseudomonas aeruginosa ATCC 27853  (10 mm) in hexane extract. It has been found that the potential of cytotoxic activity is depended on concentration and solvent type of extracts. Ethyl acetate extract showed significant inhibitory value at the concentrations of 550 μg/ml  (99.82 %) against PC-3 cell lines with IC50 264.33 (μg/ml ). Overall, the mushroom used as a daily nutrient could be a source for new drug developments and a kind of  treatment in cancer therapies, and also, organic extracts of M. esculenta may contain  substances that stimulate bioactivity.

Keywords

Morchella esculenta (L.) Pers, chemical, bioactive properties, wild mushrooms

References

• Adebayo E. A., Oloke J. K., Ayandele A. A., Adegunlola C. O., Phytochemical, antioxidant and antimicrobial assay of mushroom metabolite from Pleurotus pulmonarius–LAU 09 (JF736658), J. Microbiol. Biotech. Res.,2, 2, 366-374 (2012)
• Alley, M. C., Scudiere, D. A., Monks, A., Hursey, M. L., Czerwinski, M. J., Fine, D. L., … Boyd, M. R., Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Research, 48, 589–601(1988).
• AOAC 993.21, Total Dietary Fiber in Foods and Food Products J.AOAC İnt. 77,687 (1994).
• AOAC,Officialmethods of analysis (15 th ed.). Virginia DC, USA: Association of agriculturalchemist, 746-780 pp. (1990).
• Bobek P., Ozdín L., Galbavý Š., Dose-and time-dependent hypocholesterolemic effect of oyster mushroom (Pleurotus ostreatus) in rats, Nutrition, 14, 3, 282- 286 (1998).
• Blois, M.S. Antioxidant determinations by the use of a stable free radical, Nature, 181: 1199- 1200 (1958).
• Choi, Y.H., Huh M.K., Ryu C.H., Choi B.T., Jeong Y.K., Induction of apoptotic cell death by mycelium extracts of Phellinus linteus in human neuroblastoma cells. Int. J. Mol. Med., 14(2): 227-232 (2004).
• Das A.J., Khawas, P., Miyaji, T., Deka, S.C., HPLC and GC-MS analyses of organicacids, carbohydrates, amino acids, and volatile aromatic compounds in some varieties of rice beer from North East India, J. Inst. Brew. 120: 244–252 (2014).
• Dapkevicius, A, Venskutonis R, Van-Beek TA and Linssen PH., Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture 77(1): 140–146 (1998).
• Dinis T.C.P, Madeira V.M.C., Almeida L.M., Action of phenolic derivates (acetoaminophen, salycilate and 5- aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archive of Biochemistry and Biophysics 315(1): 161–169 (1994).
• Du, X.H., Zhao, Q., O’Donnell, K., Rooney, A.P., Yang, Z.L., Multigene molecular phylogenetics reveals true morels (Morchella) are especially species-rich in China. Fungal Genet. Biol. 49, 455–469 (2012).
• Elmastas, M., Isildak, O., Turkekul, I., Temu, N., Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J. Food Compos. Anal. 20, 337–345 (2007).
• Ergün A, Çolpan İ, Yıldız G, Küçükersan S, Tuncer S.D, Yalçın S, Küçükersan M.K, Şehu A., Yemler, Yem Hijyeni ve Teknolojisi, Ankara Üniversitesi Veteriner Fakültesi Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı. II. Baskı, Bölüm 8, 354- 391 s., ISBN 975-97808-0-1 (2004).
• Finch R., Bacterial resistance- the clinical challenge. Clin. Microbiol. Infect. 8 (Suppl 3): 21-32 (2002).
• Folch J, Lees M, Stanley A., Simple method for the isolation and purification of total lipids from animal tissues. J BiolChem 226:497–509 (1957).
• Fukuzawa M, Yamaguchi R, Hide I, Chen Z, Hirai Y, Sugimoto, A, Yasuhara T, Nakata Y., Possible involvement of long chain fatty acids in the spores of Ganoderma lucidum (Reishi Houshi) to its anti-tumor activity. Biol. Pharm. Bull., 31(10): 1933-1937 (2008).
• Genc¸ celep, H., Uzun, Y., Tuncturk, Y., Demirel, K., Determination of mineral content of wild-grown edible mushrooms. Food Chem. 113, 1033– 1036 (2009).
• Gursoy N . Sarikurkcu C., Cengiz, M., Solak M. H., Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species / Food and Chemical Toxicology 47 2381–2388 (2009).
• Güler, P., Morchella conica Pers. ve Morchella esculenta Pers. ex St. Amans’nın Fruktifikasyon Oluşumunda Bazı Kültürel Parametrelerin İncelenmesi. Hacettepe Üniversitesi, Fen Bilimleri Enstitüsü. Doktora Tezi. S.87 (1993).
• Harbarth S, Samore M.H., Antimicrobial resistance determinants and future control. Emerg. Infect. Dis., 11(6): 794-801 (2005).
• Hu H, Ahn N.S, Yang X, Lee Y.S., Kang KS Ganoderma lucidum extract induces cell cycle arrest and apoptosis in MCF-7 human breast cancer cell. Int. J. Cancer., 102(3): 250-253 (2002).
• Hur, J.-M., Yang, C.-H., Han, S.-H., Lee, S.-H., You, Y.-O., Park, J.-C., Kim, K.-J., Antibacterial Effect of Phellinus linteus Against Methicillin-Resistant Staphylococcus aureus Fitoterapia, 75:603– 605 (2004).
• İshikawa N.K, Kasuya M.C.M, Vanetti M.C.D., Antibacterial activity of Lentinula edodes grown in liquid medium. Braz J Microbiol. 32(3):206–10. doi: 10.1590/S1517-83822001000300008 (2001).
• Jayakumar T., Aloysius Thomas P., Geraldine P., Protective effect of an extract of the oyster mushroom, Pleurotus ostreatus, on antioxidants of major organs of aged rats, Experimental gerontology, 42, 3, 183-191 (2007).
• Kaçar, S., Başhan, M. Oymak.A.S., Effect of seasonal variation on lipid and fatty acid profile in muscle tissue of male and female Silurustriostegus.. Journal of food science and technology-Mysore. 53 (7): 2913–2922 (2016).Kanwal, H.K., Reddy, M.S., The effect of carbon and nitrogen sources on the formation of sclerotia in Morchella spp. Ann. Microbiol. 62, 165–168 (2012).
• Karaboz, İ., Öner, M., Batık kültürde üretilen Morchella conica var. costata miselyumunun kimyasal yapısı ve tek hücre protein (THP) olarak değerlendirilmesi: Doğa TU. Biyol (Genetik, Mikrobiyoloji, Moleküler Biyoloji, Sitoloji) D. 12(3): 190-196 (1988).
• Kavanagh F., Hervey A., Robbins W. J. , Antibiotic Substances From Basidiomycetes: VIII. Pleurotus Multilus (Fr.) Sacc. and Pleurotus Passeckerianus Pilat, Proceedings of the National Academy of Sciences of the United States of America, 37. 9. 570 (1951).
• Kavishree, S., Hemavathy, J., Lokesh, B.R., Shashirekha, M.N., Rajarathnam, S., Fat and Fatty Acids of Indian Edible Mushrooms. Food Chemistry, Volume 106, Issue 2, Pages 597-602 (2008).
• Khatua, S., Paul, S., & Acharya, K. , Mushroom as the potential source of new generation of antioxidant: a review, Research Journal of Pharmacy and Technology, 6, 5, 496-505 (2013).
• Kırbağ S., Korkmaz V, Değişik Tarımsal Atıkların Bazı Kültür Mantarı Türlerinin Besin DeğerleriÜzerine Etkisi, Artvin ÇoruhÜniversitesi Orman Fakültesi Dergisi, Cilt: 15, Sayı:2, Sayfa: 126-131 Ekim 2014 (http://ofd.artvin.edu.tr/download/article-file/25881) (2014).
• Kok T.M.C.M., Zwingman I., Moonen E.J., Schilderman P.A.E.L., Rhijnsburger E., Haenen G.R.M.M., Kleinjans J.C.S., Analysis of oxidative DNA damage after 25 human dietary supplementation with linoleic acid. Food Chem Toxicol 41:351- 358 (2003).
• Kosanić M., Ranković B., Dašić M., Mushrooms as possible antioxidant and antimicrobial agents, Iranian Journal of Pharmaceutical Research, 11, 4, 1095- 1102 (2012).
• Künsch U, Scharer H, Conedera M, Sassella A, Jermini M, Jelmini G, Quality assessment of chestnut fruits. Acta Horticulturae 494:119-127 (1999).
• Livermore D., Minimising antibiotic resistance. Lancet Infect. Dis., 5(7): 450-459 (2005).
• Lord C.J., Ashworth A., Biology-driven cancer drug development: back to the future. BMC Biol., 8:38. Doi:10.1186/1741-7007-8-38 (2010).
• O’Donnell, K., Rooney, A.P., Mills, G.L., Kuo, M., Weber, N.S., Rehner, S.A., Phylogeny and historical biogeography of true morels (Morchella) reveals an early Cretaceous origin and high continental endemism and provincialism in the Holarctic. Fungal Genet. Biol. 48, 252–265. (2011).
• Oyaizu M., Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nurition 44(6): 307–315 (1986).
• Pilz, D., McLain, R., Alexander, S., Villarreal-Ruiz, L., Berch, S., Wurtz, T.L., Parks, C.G., McFarlane, E., Baker, B., Molina, R., Smith, J.E., Ecology and Management of Morels Harvested from the Forests of Western North America. General Technical Report PNW-GTR-710. US Department of Agriculture, Forest Service, Pacific Northwest Research Station, Portland (2007).
• Preeti, A., Pushpa S., Sakshi S., Jyoti A., Antioxidant mushrooms: a review, Int Res J Pharmacy, 3, 6, 65-70 (2012).
• Sheena, N., Ajith, T.A., Mathew, A. and Janardhanan, K.K., Antibacterial Activity of Three Macrofungi, Ganoderma lucidum, Navesporus floccosa and Phellinus rimosus Occurring in South India. Pharmaceutical Biology, Vol. 41, No:8, Pages 564-567 (2003).
• Simopoulos AP, Omega-3 fatty acids in health and disease and in growth and development. Am J Clin Nutr 54:438-463 (1991).
• Szefer, P., Mineral components in foods. In: Szefer, P., Nriagu, J.O. (Eds.), Chemometric Techniques in Analytical Evaluation of Food Quality. CRC Press; Taylor & Francis, London) (2007).Taskin, H., Büyükalaca, S., Dogan, H.H., Rehner, S.A., O’Donnell, K., A multigene molecular phylogenetic assessment oftrue morels (Morchella)in Turkey. Fungal Genet. Biol. 47, 672–682 (2010).
• Üstün O., Makrofungusların besin değeri ve biyolojik etkileri. Türk Hijyen ve Deneysel Biyoloji Dergisi cilt 68, 4,223-240 (2011).
• Vieira, V., Barros, L., Martins , A., Ferreira, I. C. F. R. Nutritional and Biochemical Profiling of Leucopaxillus candidus (Bres.) Singer Wild Mushroom, Molecules , 21(1), 99; doi:10.3390/molecules21010099 (2016).
• Wong, Y.J., Chye, F.Y., Antioxidant properties of selected tropical wild edible mushrooms. J. Food Compos. Anal. 22, 269–277 (2009).
• Zhishen J., Mengcheng T., Jianming W., The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4): 555–559 (1999).