In vitro Antimicrobial Activity of Desarmillaria tabescens

Year 2019, Volume: 10 Issue: 3, 10 - 16, 26.12.2019

Kerem Canlı Atakan Benek Merve Şenturan İlgaz Akata Ergin Murat Altuner

Abstract

Mushrooms are known to be nutritive and medicinal food
stuff, which are good sources of some vitamins and essential minerals. They
also contain some therapeutic agents, thus they have been used against several
health problems for hundreds of years. The aim of this study is to determine
the in vitro antimicrobial activity
of Desarmillaria tabescens (Scop.)
R.A. Koch & Aime 2017.

D. tabescens samples were air dried and
extracted by using ethanol. Antimicrobial activity of D. tabescens ethanol
extracts were investigated against several Gram positive and Gram negative
bacteria strains, fungal strains, which are either standard or isolated from
food and some multi drug resistant (MDR) clinical isolate bacteria namely, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocua, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 5071, Pseudomonas fluorescence P1, Salmonella
enteritidis ATCC 13075, Salmonella
infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus (MDR), Escherichia coli (MDR), Klebsiella pneumoniae (MDR), Acinetobacter baumannii (MDR) and Streptococcus pneumoniae (MDR) by using
the disk diffusion method.

As a result, it was observed that ethanol extracts of D. tabescens
has low to medium antimicrobial activity against several Gram positive and Gram
negative microorganisms tested. The antimicrobial activity of D. tabescens
especially observed against K. pneumoniae
(MDR) and S. pneumoniae (MDR) is
found to be remarkable.

Keywords

Desarmillaria tabescens, antimicrobial activity, disk diffusion, multi drug resistant bacteria, MDR

Desarmillaria tabescens’in in vitro Antimikrobiyal Aktivitesi

Abstract

Mantarların,
bazı vitaminlerin ve temel minerallerin kaynağı olan besleyici ve tıbbi gıda
maddeleri olduğu bilinmektedir. Ayrıca bazı terapötik maddeler içerirler, bu
yüzden yüzlerce yıldır çeşitli sağlık problemlerine karşı kullanılmıştır. Bu
çalışmanın amacı Desarmillaria tabescens
(Scop.) R.A. Koch & Aime 2017’nin in
vitro antimikrobiyal aktivitesini belirlemektir.

D. tabescens
örnekleri kurutulmuş ve etanol kullanılarak ekstre edilmiştir. D. tabescens etanol ekstraktlarının
antimikrobiyal aktivitesi, Bacillus
subtilis DSMZ 1971, Candida albicans
DSMZ 1386, Enterobacter aerogenes
ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocua, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 5071, Pseudomonas fluorescence P1, Salmonella
enteritidis ATCC 13075, Salmonella
infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus (MDR), Escherichia coli (MDR), Klebsiella pneumoniae (MDR), Acinetobacter baumannii (MDR) ve Streptococcus pneumoniae (MDR) gibi Gram
pozitif ve Gram negatif standart, gıdadan izole edilmiş bakteri suşları, mantar
suşları ve klinik izole bazı çoklu ilaca dirençli (MDR) klinik izolat
bakteriler kullanılarak disk difüzyon yöntemini ile araştırılmıştır.

Sonuç
olarak, D. tabescens'in etanol
ekstraktlarının, test edilen bazı Gram pozitif ve Gram negatif mikroorganizmaya
karşı düşük ila orta antimikrobiyal aktiviteye sahip olduğu gözlenmiştir. D. tabescens'in özellikle K. pneumoniae (MDR) ve S. pneumoniae'ye (MDR) karşı gözlenen
antimikrobiyal aktivitesinin dikkat çekici olduğu bulunmuştur.

Keywords

Desarmillaria tabescens, antimikrobiyal aktivite, disk difüzyon, çoklu ilaca dirençli bakteriler, MDR

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