Preliminary Study on the Determination of Heat Resistant Fungi in Agricultural Soils

Year 2019, Volume: 10 Issue: 3, 60 - 66, 26.12.2019

Suat Sezen , Rasime Demirel

Abstract

Heat
resistant fungi are capable of surviving temperature at short heat
applications. These organisms continue to growing and metabolic activities.
Therefore, they cause spoilage of food products and effect on health because of
mycotoxin production. This study focused on determination of density and
biodiversity of heat resistant fungi in agricultural soil samples. For this
aim, two different soil samples were collected from agricultural lands at June
2018. Isolation process was performed by using main soil dilution method after
heat treatment at 75°C for 30 minutes of diluted soil samples. After incubation
at 25°C for 7-14 days, all colonies were purified. All of the isolates were
diagnosed by using conventional methods according to the macromorphological and
micromorphological properties. We determined 11 species belong to Alternaria, Aspergillus, Cladosporium,
Epicoccum, Humicola, Penicillium and
Taloromyces genera. As a result of
this study, we showed that agricultural soil areas have a potential for heat
resistance fungi biodiversity and exhibited that we should concentrate on this
subject with investigation of different areas and sources.

Keywords

Agricultural Soils, Heat Resistant Fungi, Conventional Method

Tarım Topraklarındaki Isıya Dirençli Fungusların Belirlenmesi Üzerine Ön Çalışma

Abstract

Isıya dayanıklı
mantarlar, kısa ısı uygulamalarına dayanabilir. Bu organizmalar büyümeye ve
metabolik aktivitelere devam ederler. Bu nedenle, gıda ürünlerinde bozulmaya ve
mikotoksin üretme yetenekleri sayesinde sağlık üzerinde etkiye neden olurlar.
Bu çalışma, tarımsal toprak örneklerinde ısıya dirençli mantarların yoğunluğu
ve biyolojik çeşitliliğinin belirlenmesi üzerine odaklanmıştır. Bu amaçla,
Haziran 2018'de tarım alanlarından iki farklı toprak örneği toplanmıştır.
İzolasyon işlemi dilüe edilmiş toprak örneklerinin 75 °C’de 30 dakika boyunca
ısı uygulaması yapıldıktan sonra toprak dilüsyon yöntemi ile
gerçekleştirilmiştir. 25 °C’de 7-14 günlük inkübasyondan sonra tüm koloniler
saflaştırılmıştır. İzolatların tamamı makromorfolojik ve mikromorfolojik
özelliklere göre geleneksel yöntemler kullanılarak teşhis edilmiştir. Alternaria, Aspergillus, Cladosporium,
Epicoccum, Humicola, Penicillium ve Taloromyces
cinslerine ait 11 tür tespit edilmiştir. Bu çalışma sonucunda, tarımsal toprak
alanlarının ısıya dirençli mantar biyolojik çeşitliliği potansiyeline sahip
olduğunu belirledik ve farklı alanların ve kaynakların araştırılmasıyla bu
konuya odaklanmamız gerektiğini gösterdik.

Keywords

Tarım Toprağı, Isıya Dirençli Fungus, Geleneksel Yöntem

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