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Utilization of Agricultural and Forestry by-Products in Ganoderma lucidum (Curt.: Fr.) P. Karst. Production

Year 2022, Volume: 13 Issue: 3, 1 - 8, 30.12.2022
https://doi.org/10.30708/mantar.1185553

Abstract

The aim of the study was to evaluate the use of some local agricultural and forestry by-products as Ganoderma lucidum growing subsrate. In the study, 8 different growing media prepared by mixing agricultural and forestry wastes such as oak sawdust (OS), poplar sawdust (PS), wheat straw (WS), wood chips (WCH), green almond hulls (AH) and green walnut hulls (GWH) alone or in different proportions, were used for G. lucidum cultivation and the effects of these substrates on spawn run time (SRT), days to pinhead initiation (DPI), days to the first harvest (DFH), yield (g/kg) and biological efficiency (BE%) were investigated. The results demonstrated that spawn run time (SRT) of G. lucidum was shorter in PS and WS:GWH12.5 (19.7 days and 20.8 days) as compared to other substrates and mixtures (22.2-28.5 days). The pinheads emerged in substrates between 43th- 49th days and the fruiting bodies were collected between 80.3 -93.5 days after the spawning. Total fresh mushroom yield and BE(%) varied from 71.39 to 110.10 g/kg of substrate and from 21.0% to 31.46, respectively. Moreover, PS substrate produced fruitbody also earlier compared with the other investigated substrates. The study revealed that although higher total yields were obtained by sawdust substrates, considering the difficulty of finding sawdust in some areas, WS and the mixture of WS and GHW (87.5:12.5%) could be also successfully used inof G. lucidum production.

References

  • Amiri-Sadeghan, A., Aftabi, Y., Arvanaghi, H.R., Shokri, E., Khalili, M., Seyedrezazadeh, E., and Kuhare, F. (2022). A Review of Substrates for Solid-State Fermentation of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), for Basidiome Production and Effect on Bioactive Compounds. Int. J. Med. Mushrooms, 24(4), 15 – 29
  • Atila, F. (2017) Evaluation of Suitability of Various Agro-Wastes for Productivity of Pleurotus djamor, Pleurotus citrinopileatus and Pleurotus eryngii Mushrooms. J Exp Agric Int 17: 1–11. https://doi.org/10.9734/JEAI/2017/36346
  • Atila, F. (2019). The Use of Phenolic-rich Agricultural Wastes for Hericium erinaceus and Lentinula edodes Cultivation. Ege Üniv. Ziraat Fak. Derg. 56(4), 417-425. https://doi.org/10.20289/zfdergi.446022
  • Atila, F. (2020). Comparative Study on the Mycelial Growth and Yield of Ganoderma lucidum (Curt.: Fr.) Karst. on Different Lignocellulosic Wastes. Acta Ecol. Sin. 40(2), 153-157 https://doi.org/10.1016/j.chnaes.2018.11.007
  • A.O.A.C. (2019) Official Methods of Analysis of AOAC International [www Document]. 21st Ed. https://www.aoac.org/aoac_prod_imis/AOAC/Publications/Official_Methods_of_Analysis/AOAC_Member/Pubs/OMA/AOAC_Official_Methods_of_Analysis.aspx
  • Chan, S.W., Tomlinson, B., Chan, P. and Lam, C.W.K. (2021). The Beneficial Effects of Ganoderma lucidum on Cardiovascular And Metabolic Disease Risk, Pharm. Biol. 59(1), 1159-1169. https://doi.org/10.1080/13880209.2021.1969413
  • Cilerdzic, J., Stajic, M.M. and Vukojevic, J.B. (2017). Ganoderma lucidum – from Tradition to Modern Medicine. Matica Srpska J. Nat. Sci. Novi. Sad. 133, 151-161. https://doi.org/10.2298/ZMSPN1733151C Cör D, Knez Ž and Knez Hrnčič M. (2018). Antitumour, Antimicrobial, Antioxidant and Antiacetylcholinesterase Effect of Ganoderma lucidum Terpenoids and Polysaccharides: A Review. Molecules. 23(3), 649. https://doi.org/10.3390/molecules23030649
  • Gurung, O.K., Budathoki, U. and Parajuli, G. (2012). Effect of Different Substrates on the Production of Ganoderma lucidum (Curt: Fr) Karst. Our Nature. 10, 191- 198.
  • Hal, Y. B., Yarar, M., Kara, E., Baktemur, G. and Taşkın, H. (2021). Farklı Tarımsal Atıkların Ganoderma lucidum (Reishi mantarı) Yetiştiriciliğinde Verim ve Kalite Üzerine Etkisi. Çukurova J. Agric. Food Sci. 36(1), 275-288. https://doi.org/10.36846/CJAFS.2021.55
  • Hsieh, C., Yang and F. C. (2004). Reusing Soy Residue for the Solid-State Fermentation of Ganoderma lucidum. Bioresource Technol 91, 105-109. https://doi.org/10.1016/s0960-8524(03)00157-3
  • Karma, A. and Bhatt, A.B. (2013) First Attempt of an Organic Cultivation of Red Ganoderma Lucidum Under Subtropical Habitat and Its Economics. Int. J. Pharm. Sci. 5, 94–98
  • Khoo, S.C., Ma, N.L., Peng, W.X., Ng, K.K., Goh, M.S., Chen, H.L., Tan, S.H., Lee, C.H., Luang-In, V. and Sonne, C. (2022). Valorisation of Biomass and Diaper Waste into A Sustainable Production of The Medical Mushroom Lingzhi Ganoderma lucidum, Chemosphere. 286(1), 131477. https://doi.org/10.1016/j.chemosphere.2021.131477.
  • Koutrotsios, G., Patsou, M., Mitsou, E.K., Bekiaris, G., Kotsou, M., Tarantilis, P.A., Pletsa, V., Kyriacou, A. and Zervakis, G.I. (2019). Valorization of Olive By-Products as Substrates for the Cultivation of Ganoderma lucidum and Pleurotus ostreatus Mushrooms with Enhanced Functional and Prebiotic Properties. Catalysts. 9(6), 537. https://doi.org/10.3390/catal9060537
  • Li, S., Dong, C., Wen, H.A. and Liu, X. (2016). Development of Ling-zhi industry in China–emanated from the Artificial Cultivation in the Institute of Microbiology, Chinese Academy of Sciences (IMCAS). Mycology. 7, 74–80
  • Liu, Q. and Tie, L. (2019). Preventive and Therapeutic Effect of Ganoderma (Lingzhi) on Diabetes. In: Lin, Z., Yang, B. (eds) Ganoderma and Health. Advances in Experimental Medicine and Biology, 1182. Springer, Singapore. https://doi.org/10.1007/978-981-32-9421-9_8
  • Mehta, S., Jandaik, S. and Gupta, D. (2014). Effect of Cost-Effective Substrates on Growth Cycle and Yield of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) from Northwestern Himalaya (India). Int. J. Med. Mushrooms. 16(6), 585–591
  • Ozcariz-Fermoselle, M.V., Fraile-Fabero,R., Girbés-Juan,T., Arce-Cervantes, O., Rueda-Salgueiro, J.A.O. and Azul, A.M. (2018). Use Of Lignocellulosic Wastes of Pecan (Carya illinoinensis) in the Cultivation of Ganoderma lucidum, Rev. Iberoam. Micol. 35(2), 103-109. https://doi.org/10.1016/j.riam.2017.09.005.
  • Peksen, A. and Yakupoglu, G. (2009). Tea Waste as a Supplement for the Cultivation of Ganoderma lucidum. World J. Microbiol. Biotechnol. 25, 611–618. Rashad, F.M.,. El Kattan, M.H., Fathy, H.M., Abd El-Fattah, D.A., El Tohamy, M. and Farahat, A.A. (2019). Recycling of Agro-Wastes for Ganoderma lucidum Mushroom Production and Ganoderma Post Mushroom Substrate As Soil Amendment. Waste Manage. 88, 147–159
  • Ren, L., Zhang, J. and Zhang, T. (2021). Immunomodulatory activities of polysaccharides from Ganoderma on immune effector cells. Food Chem. 340, 127933. https://doi.org/10.1016/j.foodchem.2020.127933.
  • Rolim, L.N., Sales-Campos, C., Cavalcanti, M.A.Q. and Urben, A.F. (2014). Application of Chinese Jun-Cao Technique for the Production of Brazilian Ganoderma lucidum Strains. Braz. Arch. Biol. Technol. 57, 367-373
  • Sohretoglu, D. and Huang, S. (2018). Ganoderma lucidum Polysaccharides as An Anti-cancer Agent. Anti-Cancer Agents Med. Chem. 18(5), 667-674. http://dx.doi.org/10.2174/1871520617666171113121246
  • Stampar, F., Solar, A., Hudina, M., Veberic, R. and Colaric, M. (2006) Traditional Walnut Liqueur -Cocktail of Phenolics. Food Chem 95, 627–631. https://doi.org/10.1016/j.foodchem.2005.01.035
  • Terzi, I. (2008) Allelopathic Effects of Juglone and Decomposed Walnut Leaf Juice on Muskmelon and Cucumber Seed Germination and Seedling Growth. Afr, J. Biotech. 7, 1870–1874
  • Tiquia, S.M. and Tam, N.F.Y. (2000) Fate of Nitrogen During Composting of Chicken Litter. Environ. Pollut. 110, 535–541. https://doi.org/10.1016/s0269-7491(99)00319-x
  • Xu, J., Xiao, C.M., Xu, H.S., Yang, S.X., Chen, Z.M., Wang, H.Z., Zheng, B.S., Mao, B.Z. and Wu, X.Q., 2021. Anti-inflammatory effects of Ganoderma lucidum sterols via attenuation of the p38 MAPK and NF-κB pathways in LPS-induced RAW 264.7 macrophages. Food and Chemical Toxicology.150, 112073. https://doi.org/10.1016/j.fct.2021.112073
  • Vansoest, P.J., Robertson, J.B. and Lewis, B.A., 1991. Method for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74 (10), 3583–3597.
  • Wachtel-Galor, S., Yuen, J., Buswell, J.A. and Benzie, I.F.F. (2011). Herbal Medicine: Biomolecular and Clinical Aspects. 2nd Edition, CRC Press, Florida. Yu, Y.N. and Shen, M.Z., 2003. The history of Lingzhi (Ganoderma spp.) cultivation. Mycosyst. 22, 3–9.
  • Zadrazil, F. and Brunnert, H. (1982). Solid State Fermentation of Lignocellulose Containing Plant Residues With Sporotrichum pulverlentium. Nov. and Dichotimus squalens (Karsl) Reid. Eur. J. Appl. Microbiol. Biotech. 16, 45–51.
  • Zhang, H., Gao, J.M., Liu, W.T., Tang, J.C., Zhang, X.C., Jin, Z.G., Xu, Y.P. and Shao, M.A. (2008). Allelopathic Substances from Walnut (Juglans regia L) Leaves. Allelopathy J. 21 (2), 354–362.
  • Zhao, S., Rong, C., Gao, Y., Linfeng Wu, Luo, X., Song, S. et al. (2021). Antidepressant-Like Effect of Ganoderma lucidum Spore Polysaccharide-Peptide Mediated by Upregulation of Prefrontal Cortex Brain-Derived Neurotrophic Factor. Appl. Microbiol. Biotechnol. 105, 8675–8688 https://doi.org/10.1007/s00253-021-11634-y
  • Zhou, X.W. and Deng, B.W. (1996). Research on Various Media affecting Mycelial Growth of Ganoderma. J. Hanzhong Teach. Coll: Natural Science Edition. 14, 61-64
  • Zhou, X.W., 2017. Cultivation of Ganoderma lucidum. Edible and Medicinal Mushrooms: Tech. Applications 385–413. https://doi.org/10.1002/9781119149446.ch18

Ganoderma lucidum (Curt.: Fr.) P. Karst. Üretiminde Tarım Ve Ormancılık Yan Ürünlerinin Kullanımı

Year 2022, Volume: 13 Issue: 3, 1 - 8, 30.12.2022
https://doi.org/10.30708/mantar.1185553

Abstract

Çalışmanın amacı, bazı yerel tarım ve orman yan ürünlerinin Ganoderma lucidum yetiştirme substratı olarak kullanımını değerlendirmektir. Çalışmada meşe talaşı (OS), kavak talaşı (PS), buğday samanı (WS), yonga (WCH), badem kabuğu (AH) ve yeşil ceviz kabuğu (GWH) gibi tarım ve orman atıklarının tek başına veya farklı oranlarda karıştırılmasıyla hazırlanan 8 farklı yetiştirme ortamı kullanılmıştır. G. lucidum yetiştiriciliği için kullanılmış ve bu substratların misel gelişim süresi (SRT), taslak oluşumuna kadar geçen gün sayısı (DPI), ilk hasata kadar geçen gün sayısı (DFH), verim (g/kg) ve biyolojik etkinliğe (BE%) etkileri araştırılmıştır. Sonuçlar, G. lucidum'un misel gelişim süresinin, PS ve WS:GWH12.5'te (19.7 gün ve 20.8 gün) diğer substratlara ve karışımlara (22.2-28.5 gün) kıyasla daha kısa olduğunu gösterdi. Misel ekiminden sonra 43-49. günlerde taslaklar oluştu ve G. lucidum'un şapkaları misel ekiminden 80.3-93.5 gün sonra hasat edildi. Toplam taze mantar verimi ve BE(%) sırasıyla 71.39 g/kg ila 110.10 g/kg substrat ve %21.0 ila %31.46 arasında değişmiştir. Ayrıca, PS substratında araştırılan diğer substratlarla karşılaştırıldığında hasat daha erken gerçekleşti. Çalışma, talaş substratlardan daha yüksek toplam verim elde edilmesine rağmen, bazı bölgelerde talaş bulmanın zorluğu göz önüne alındığında, WS'nin WS ve GHW karışımının da (%87.5:%12.5) G. lucidum'un yetiştirilmesi için başarıyla kullanılabileceğini ortaya koydu.

References

  • Amiri-Sadeghan, A., Aftabi, Y., Arvanaghi, H.R., Shokri, E., Khalili, M., Seyedrezazadeh, E., and Kuhare, F. (2022). A Review of Substrates for Solid-State Fermentation of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), for Basidiome Production and Effect on Bioactive Compounds. Int. J. Med. Mushrooms, 24(4), 15 – 29
  • Atila, F. (2017) Evaluation of Suitability of Various Agro-Wastes for Productivity of Pleurotus djamor, Pleurotus citrinopileatus and Pleurotus eryngii Mushrooms. J Exp Agric Int 17: 1–11. https://doi.org/10.9734/JEAI/2017/36346
  • Atila, F. (2019). The Use of Phenolic-rich Agricultural Wastes for Hericium erinaceus and Lentinula edodes Cultivation. Ege Üniv. Ziraat Fak. Derg. 56(4), 417-425. https://doi.org/10.20289/zfdergi.446022
  • Atila, F. (2020). Comparative Study on the Mycelial Growth and Yield of Ganoderma lucidum (Curt.: Fr.) Karst. on Different Lignocellulosic Wastes. Acta Ecol. Sin. 40(2), 153-157 https://doi.org/10.1016/j.chnaes.2018.11.007
  • A.O.A.C. (2019) Official Methods of Analysis of AOAC International [www Document]. 21st Ed. https://www.aoac.org/aoac_prod_imis/AOAC/Publications/Official_Methods_of_Analysis/AOAC_Member/Pubs/OMA/AOAC_Official_Methods_of_Analysis.aspx
  • Chan, S.W., Tomlinson, B., Chan, P. and Lam, C.W.K. (2021). The Beneficial Effects of Ganoderma lucidum on Cardiovascular And Metabolic Disease Risk, Pharm. Biol. 59(1), 1159-1169. https://doi.org/10.1080/13880209.2021.1969413
  • Cilerdzic, J., Stajic, M.M. and Vukojevic, J.B. (2017). Ganoderma lucidum – from Tradition to Modern Medicine. Matica Srpska J. Nat. Sci. Novi. Sad. 133, 151-161. https://doi.org/10.2298/ZMSPN1733151C Cör D, Knez Ž and Knez Hrnčič M. (2018). Antitumour, Antimicrobial, Antioxidant and Antiacetylcholinesterase Effect of Ganoderma lucidum Terpenoids and Polysaccharides: A Review. Molecules. 23(3), 649. https://doi.org/10.3390/molecules23030649
  • Gurung, O.K., Budathoki, U. and Parajuli, G. (2012). Effect of Different Substrates on the Production of Ganoderma lucidum (Curt: Fr) Karst. Our Nature. 10, 191- 198.
  • Hal, Y. B., Yarar, M., Kara, E., Baktemur, G. and Taşkın, H. (2021). Farklı Tarımsal Atıkların Ganoderma lucidum (Reishi mantarı) Yetiştiriciliğinde Verim ve Kalite Üzerine Etkisi. Çukurova J. Agric. Food Sci. 36(1), 275-288. https://doi.org/10.36846/CJAFS.2021.55
  • Hsieh, C., Yang and F. C. (2004). Reusing Soy Residue for the Solid-State Fermentation of Ganoderma lucidum. Bioresource Technol 91, 105-109. https://doi.org/10.1016/s0960-8524(03)00157-3
  • Karma, A. and Bhatt, A.B. (2013) First Attempt of an Organic Cultivation of Red Ganoderma Lucidum Under Subtropical Habitat and Its Economics. Int. J. Pharm. Sci. 5, 94–98
  • Khoo, S.C., Ma, N.L., Peng, W.X., Ng, K.K., Goh, M.S., Chen, H.L., Tan, S.H., Lee, C.H., Luang-In, V. and Sonne, C. (2022). Valorisation of Biomass and Diaper Waste into A Sustainable Production of The Medical Mushroom Lingzhi Ganoderma lucidum, Chemosphere. 286(1), 131477. https://doi.org/10.1016/j.chemosphere.2021.131477.
  • Koutrotsios, G., Patsou, M., Mitsou, E.K., Bekiaris, G., Kotsou, M., Tarantilis, P.A., Pletsa, V., Kyriacou, A. and Zervakis, G.I. (2019). Valorization of Olive By-Products as Substrates for the Cultivation of Ganoderma lucidum and Pleurotus ostreatus Mushrooms with Enhanced Functional and Prebiotic Properties. Catalysts. 9(6), 537. https://doi.org/10.3390/catal9060537
  • Li, S., Dong, C., Wen, H.A. and Liu, X. (2016). Development of Ling-zhi industry in China–emanated from the Artificial Cultivation in the Institute of Microbiology, Chinese Academy of Sciences (IMCAS). Mycology. 7, 74–80
  • Liu, Q. and Tie, L. (2019). Preventive and Therapeutic Effect of Ganoderma (Lingzhi) on Diabetes. In: Lin, Z., Yang, B. (eds) Ganoderma and Health. Advances in Experimental Medicine and Biology, 1182. Springer, Singapore. https://doi.org/10.1007/978-981-32-9421-9_8
  • Mehta, S., Jandaik, S. and Gupta, D. (2014). Effect of Cost-Effective Substrates on Growth Cycle and Yield of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) from Northwestern Himalaya (India). Int. J. Med. Mushrooms. 16(6), 585–591
  • Ozcariz-Fermoselle, M.V., Fraile-Fabero,R., Girbés-Juan,T., Arce-Cervantes, O., Rueda-Salgueiro, J.A.O. and Azul, A.M. (2018). Use Of Lignocellulosic Wastes of Pecan (Carya illinoinensis) in the Cultivation of Ganoderma lucidum, Rev. Iberoam. Micol. 35(2), 103-109. https://doi.org/10.1016/j.riam.2017.09.005.
  • Peksen, A. and Yakupoglu, G. (2009). Tea Waste as a Supplement for the Cultivation of Ganoderma lucidum. World J. Microbiol. Biotechnol. 25, 611–618. Rashad, F.M.,. El Kattan, M.H., Fathy, H.M., Abd El-Fattah, D.A., El Tohamy, M. and Farahat, A.A. (2019). Recycling of Agro-Wastes for Ganoderma lucidum Mushroom Production and Ganoderma Post Mushroom Substrate As Soil Amendment. Waste Manage. 88, 147–159
  • Ren, L., Zhang, J. and Zhang, T. (2021). Immunomodulatory activities of polysaccharides from Ganoderma on immune effector cells. Food Chem. 340, 127933. https://doi.org/10.1016/j.foodchem.2020.127933.
  • Rolim, L.N., Sales-Campos, C., Cavalcanti, M.A.Q. and Urben, A.F. (2014). Application of Chinese Jun-Cao Technique for the Production of Brazilian Ganoderma lucidum Strains. Braz. Arch. Biol. Technol. 57, 367-373
  • Sohretoglu, D. and Huang, S. (2018). Ganoderma lucidum Polysaccharides as An Anti-cancer Agent. Anti-Cancer Agents Med. Chem. 18(5), 667-674. http://dx.doi.org/10.2174/1871520617666171113121246
  • Stampar, F., Solar, A., Hudina, M., Veberic, R. and Colaric, M. (2006) Traditional Walnut Liqueur -Cocktail of Phenolics. Food Chem 95, 627–631. https://doi.org/10.1016/j.foodchem.2005.01.035
  • Terzi, I. (2008) Allelopathic Effects of Juglone and Decomposed Walnut Leaf Juice on Muskmelon and Cucumber Seed Germination and Seedling Growth. Afr, J. Biotech. 7, 1870–1874
  • Tiquia, S.M. and Tam, N.F.Y. (2000) Fate of Nitrogen During Composting of Chicken Litter. Environ. Pollut. 110, 535–541. https://doi.org/10.1016/s0269-7491(99)00319-x
  • Xu, J., Xiao, C.M., Xu, H.S., Yang, S.X., Chen, Z.M., Wang, H.Z., Zheng, B.S., Mao, B.Z. and Wu, X.Q., 2021. Anti-inflammatory effects of Ganoderma lucidum sterols via attenuation of the p38 MAPK and NF-κB pathways in LPS-induced RAW 264.7 macrophages. Food and Chemical Toxicology.150, 112073. https://doi.org/10.1016/j.fct.2021.112073
  • Vansoest, P.J., Robertson, J.B. and Lewis, B.A., 1991. Method for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74 (10), 3583–3597.
  • Wachtel-Galor, S., Yuen, J., Buswell, J.A. and Benzie, I.F.F. (2011). Herbal Medicine: Biomolecular and Clinical Aspects. 2nd Edition, CRC Press, Florida. Yu, Y.N. and Shen, M.Z., 2003. The history of Lingzhi (Ganoderma spp.) cultivation. Mycosyst. 22, 3–9.
  • Zadrazil, F. and Brunnert, H. (1982). Solid State Fermentation of Lignocellulose Containing Plant Residues With Sporotrichum pulverlentium. Nov. and Dichotimus squalens (Karsl) Reid. Eur. J. Appl. Microbiol. Biotech. 16, 45–51.
  • Zhang, H., Gao, J.M., Liu, W.T., Tang, J.C., Zhang, X.C., Jin, Z.G., Xu, Y.P. and Shao, M.A. (2008). Allelopathic Substances from Walnut (Juglans regia L) Leaves. Allelopathy J. 21 (2), 354–362.
  • Zhao, S., Rong, C., Gao, Y., Linfeng Wu, Luo, X., Song, S. et al. (2021). Antidepressant-Like Effect of Ganoderma lucidum Spore Polysaccharide-Peptide Mediated by Upregulation of Prefrontal Cortex Brain-Derived Neurotrophic Factor. Appl. Microbiol. Biotechnol. 105, 8675–8688 https://doi.org/10.1007/s00253-021-11634-y
  • Zhou, X.W. and Deng, B.W. (1996). Research on Various Media affecting Mycelial Growth of Ganoderma. J. Hanzhong Teach. Coll: Natural Science Edition. 14, 61-64
  • Zhou, X.W., 2017. Cultivation of Ganoderma lucidum. Edible and Medicinal Mushrooms: Tech. Applications 385–413. https://doi.org/10.1002/9781119149446.ch18
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Primary Language English
Journal Section RESEARCH ARTICLE
Authors

Funda Atila 0000-0003-1129-1045

Publication Date December 30, 2022
Published in Issue Year 2022 Volume: 13 Issue: 3

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APA Atila, F. (2022). Utilization of Agricultural and Forestry by-Products in Ganoderma lucidum (Curt.: Fr.) P. Karst. Production. Mantar Dergisi, 13(3), 1-8. https://doi.org/10.30708/mantar.1185553

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