The role of dynamic thiol/disulfide homeostasis for the evaluation of oxidative stress in endometriosis patients

Year 2022, , 143 - 147, 30.05.2022

Nura Fitnat Topbas Selcukı Pinar Yalcın Bahat Cihan Kaya Salim Neselıoglu Kubra Bagcı Mustafa Goksu Meric Kabakcı Ozcan Erel

https://doi.org/10.5472/marumj.1120611

Abstract

Objective: To evaluate the role of oxidative stress in endometriosis patients by determining dynamic thiol/disulfide homeostasis and
ischemia modified albumin (IMA) levels.

Patients and Methods: This prospective case-controlled study was conducted at a tertiary gynecology clinic in Istanbul, Turkey.
86 patients previously diagnosed with endometriosis and persistent endometriomas were included in the study group. 60 patients
who visited the clinic during the study period for routine gynecological control were included in the control group. Thiol/disulfide
parameters and IMA levels were determined from the serum samples.

Results: When the thiol/disulfide parameters were compared between the study and the control group no significant difference was
observed (p=0.49). Mean disulfide level in the control group was 18.58 ± 5.73 μmol/L and in the study group was 18.61 ± 7.37 μmol/L.
Levels were statistically similar in both groups (p=0.98). In addition, there were no differences between the groups in terms of IMA
and albumin levels.

Conclusion: The results of this study revealed no significant difference in the dynamic thiol/disulfide homeostasis among the
endometriosis patients. Although, it has been accepted as a potential oxidative stress marker in other chronic inflammatory diseases,
its use in determining the systemic oxidative stress level in endometriosis patients is limited.

Keywords

Endometriosis, Endometrioma, Dynamic thiol/disulfide homeostasis, Ischemia modified albumin

References

• [1] Zondervan KT, Becker CM, Missmer SA. Endometriosis. N Engl J Med 2020; ;382:1244-56. doi: 10.1056/NEJMra1810764
• [2] Sampson JA. Perforating hemorrhagic (chocolate) cysts of the ovary: Their importance and especially their relation to pelvic adenomas of endometrial type (Adenomyoma of the uterus, rectovaginal septum, sigmoid, etc.). Arch Surg 1921;3:245- 323. doi:10.1001/archsurg.1921.011.10080003001
• [3] Sampson JA. Metastatic or embolic endometriosis, due to the menstrual dissemination of endometrial tissue into the venous circulation. Am J Pathol 1927;3:93-110.43. PMID: 19969738
• [4] Vinatier D, Orazi G, Cosson M, Dufour P. Theories of endometriosis. Eur J Obstet Gynecol Reprod Biol 2001;96:21- 34. doi: 10.1016/s0301-2115(00)00405-x
• [5] Foyouzi N, Berkkanoglu M, Arici A, Kwintkiewicz J, Izquierdo D, Duleba AJ. Effects of oxidants and antioxidants on proliferation of endometrial stromal cells. Fertil Steril 2004;82 Suppl 3:1019-22. doi: 10.1016/j.fertnstert.2004.02.133
• [6] Augoulea A, Mastorakos G, Lambrinoudaki I, Christodoulakos G, Creatsas G. The role of the oxidative-stress in the endometriosis-related infertility. Gynecol Endocrinol 2009;25:75-81. doi: 10.1080/095.135.90802485012
• [7] Christodoulakos G, Augoulea A, Lambrinoudaki I, Sioulas V, Creatsas G. Pathogenesis of endometriosis: The role of defective ‘immunosurveillance.’ Eur J Contracept Reprod Health Care 2007;12:194-202. doi: 10.1080/136.251.80701387266
• [8] Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol RBE. 2005 Jul;3:28. doi: 10.1186/1477-7827-3-28
• [9] Gupta S, Goldberg JM, Aziz N, Goldberg E, Krajcir N, Agarwal A. Pathogenic mechanisms in endometriosis-associated infertility. Fertil Steril 2008;90:247-57. doi: 10.1016/j. fertnstert.2008.02.093
• [10] Gumusyayla S, Vural G, Bektas H, Deniz O, Neselioglu S, Erel O. A novel oxidative stress marker in patients with Alzheimer’s disease: dynamic thiol-disulphide homeostasis. Acta Neuropsychiatr 2016 ;28:315-20. doi: 10.1017/neu.2016.13
• [11] Erel O, Neselioglu S. A novel and automated assay for thiol/ disulphide homeostasis. Clin Biochem 2014;47:326-32. doi: 10.1016/j.clinbiochem.2014.09.026
• [12] Jones DP, Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radic Biol Med 2009;47:1329-38. doi: 10.1016/j.freeradbiomed.2009.08.021
• [13] Bar-Or D, Lau E, Winkler JV. A novel assay for cobaltalbumin binding and its potential as a marker for myocardial ischemia-a preliminary report. J Emerg Med 2000;19:311-5. doi: 10.1016/s0736-4679(00)00255-9
• [14] Bozkaya G, Karaca I, Fenercioglu O, Yildirim Karaca S, Bilgili S, Uzuncan N. Evaluation of maternal serum ischemia modified albumin and total antioxidant status in ectopic pregnancy. J Matern Fetal Neonatal Med 2019;32:2003-8. doi: 10.1080/14767.058.2017.1422718
• [15] Menon B, Ramalingam K, Krishna V. Study of ischemia modified albumin as a biomarker in acute ischaemic stroke. Ann Neurosci 2018;25:187-90. doi: 10.1159/000488188
• [16] Borderie D, Allanore Y, Meune C, Devaux JY, Ekindjian OG, Kahan A. High ischemia-modified albumin concentration reflects oxidative stress but not myocardial involvement in systemic sclerosis. Clin Chem 2004;50:2190-3. doi: 10.1373/ clinchem.2004.034371
• [17] Yazıcı MU, Ayar G, Savas-Erdeve S, et al. Role of ischemia modified albumin serum levels as an oxidative stress marker in children with diabetic ketoacidosis. Comb Chem High Throughput Screen 2019;22:577-81. doi: 10.2174/138.620.7322666.191.008214919
• [18] Seneş M, Kazan N, Coşkun O, Zengi O, Inan L, Yücel D. Oxidative and nitrosative stress in acute ischaemic stroke. Ann Clin Biochem 2007;44(Pt 1):43-7. doi: 10.1258/000.456.307779596057
• [19] Exacoustos C, Manganaro L, Zupi E. Imaging for the evaluation of endometriosis and adenomyosis. Best Pract Res Clin Obstet Gynaecol 2014;28:655-81. doi: 10.1016/j. bpobgyn.2014.04.010
• [20] Vignali M, Belloni GM, Pietropaolo G, et al. Effect of dienogest therapy on the size of the endometrioma. Gynecol Endocrinol 2020;36:723-7. doi: 10.1080/09513.590.2020.1725965
• [21] Van Langendonckt A, Casanas-Roux F, Donnez J. Oxidative stress and peritoneal endometriosis. Fertil Steril 2002;77:861- 70. doi: 10.1016/s0015-0282(02)02959-x
• [22] Ngô C, Chéreau C, Nicco C, Weill B, Chapron C, Batteux F. Reactive oxygen species controls endometriosis progression. Am J Pathol 2009;175:225-34. doi: 10.2353/ajpath.2009.080804
• [23] Carvalho LFP, Samadder AN, Agarwal A, Fernandes LFC, Abrão MS. Oxidative stress biomarkers in patients with endometriosis: systematic review. Arch Gynecol Obstet 2012 ;286:1033-40. doi: 10.1007/s00404.012.2439-7
• [24] Ota H, Igarashi S, Tanaka T. Xanthine oxidase in eutopic and ectopic endometrium in endometriosis and adenomyosis. Fertil Steril 2001;75:785-90. doi: 10.1016/s0015-0282(01)01670-3
• [25] Murphy AA, Santanam N, Parthasarathy S. Endometriosis: a disease of oxidative stress? Semin Reprod Endocrinol 1998;16:263-73. doi: 10.1055/s-2007.101.6286
• [26] Kamada Y, Nakatsuka M, Asagiri K, et al. GnRH agonistsuppressed expression of nitric oxide synthases and generation of peroxynitrite in adenomyosis. Hum Reprod Oxf Engl 2000;15:2512-9. doi: 10.1093/humrep/15.12.2512
• [27] Lambrinoudaki IV, Augoulea A, Christodoulakos GE, et al. Measurable serum markers of oxidative stress response in women with endometriosis. Fertil Steril 2009;91:46-50. doi: 10.1016/j.fertnstert.2007.11.021.
• [28] Fidan F, Alkan BM, Uğurlu FG, et al. Dynamic thiol/disulphide homeostasis in Patients with fibromyalgia. Arch Rheumatol 2017;32:112-7. doi: 10.5606/ArchRheumatol.2017.5931
• [29] Amreen S, Kumar P, Gupta P, Rao P. Evaluation of oxidative stress and severity of Endometriosis. J Hum Reprod Sci 2019;12:40-6. doi: 10.4103/jhrs.JHRS_27_17