Antioxidant, antimicrobial activities and total flavonoid contents of Cirsium bulgaricum DC. leaf extracts

Year 2015, Volume: 19 Issue: 1, 43 - 51, 09.01.2015

Ayça Karasakal , A.şükrü Demirci Nazan Tokatlı Demirok , Evren Cabı

Abstract

In this study, the antioxidant activities of methanol and
acetone extracts of Cirsium bulgaricum DC. (Asteraceae)
were evaluated by five antioxidant assays, including
phosphomolybdate method, cupric ion reducing capacity
(CUPRAC), 2,2’-azinobis(3-ethylbenzothiazoline-6-
sulfonicacid)/persulfate) ABTS/Persulfate, N, N-dimethyl-pphenylenediamine
(DMPD) and a Ce(IV)-based reducing
capacity (CERAC) methods. The 80% methanol and acetone
extracts showed 0.005-6.16±0.03 mmol/g and 0.004-
5.02±0.02 [(trolox (TR), catechin (CT), epicatechin (EC),
rutin (RT), quercetin (QR), naringin (NG) in CUPRAC and
ABTS/Persulfate methods, respectively]. According to DMPD
methods, inhibition effect were showed 2.3%±0.02 for 80%
methanol and 4.6%±0.07 for acetone. Ascorbic acid equivalent
mmol (AA)/g extract in CERAC method was found to be
0.0834±0.09 for 80% methanol and 0.5622±0.05 for acetone.
Ascorbic acid equivalent μg ascorbic acid (AA) /mL extract
were found 807.6±0.07 for 80% methanol and 292.4±0.01 for
acetone in phpsphomolybdate method. In the addition to the
antioxidant activity of the extract, the total flavonoid content
was measured. Total flavonoid content of 80% methanol and
acetone extracts was found to be 105.5-645±0.04 μg/ml
quercetin and rutin equivalent. For the determination of
antibacterial activities Escherichia coli (E.coli) ATCC 25922,
Escherichia coli(E.coli) O157:H7 ATCC 33150, Salmonella
E n t e r i t i d i s(S.E n t e r i t i d i s ) AT C C 1 3 0 7 6 , Listeria
monocytogenes(L.monocytogenes) AT C C 7 6 4 4 ,
Staphylococcus aureus(S.aureus) ATCC 25923 bacteria were
used as test bacteria. Acetone extracts showed antibacterial
activity against all of the tested bacteria ranged between
17.00±1.72- 19.88±1.75 mm and 80% methanol extracts
showed antibacterial activity against all of the tested bacteria
ranged between 11.35±1.43-14.43±1.32 mm.
Keywords: Antioxidant Activity, Antimicrobial Activity,
Total Flavonoid Contents, Cirsium bulgaricum

Keywords

Antioxidant Activity, Antimicrobial Activity

ORIGINAL RESEARCH

Abstract

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