First molecular record of the alien species Pacific oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey

Year 2020, Volume: 9 Issue: 1, 23 - 31, 30.06.2020

Emel Özcan Gökçek , Sefa Acarlı , Bilge Karahan , Pervin Vural , Evren Koban Baştanlar

https://doi.org/10.33714/masteb.668529

Cited By: 7

Abstract

The Pacific oyster (Crassostrea
gigas) has a very important economic potential for aquaculture, but on the
other hand, is among the highly invasive species in the world and within the
Mediterranean ecosystem. In the 1960s, C. gigas was brought to Europe
for aquaculture in the Mediterranean and Black Sea regions from Japan and
Canada. The Turkish waters are the part of the Mediterranean Sea, which is the
world’s most invaded sea. The invasion of alien species results from marine
transportation and aquaculture activities of non-native species. A heavy
maritime traffic is also present in the Marmara Sea, which connects the Black
Sea and Mediterranean Sea. The identification of the invasive species and their
distributions is very prominent in terms of protecting natural habitat and
monitoring the effects of invasive species. In this study, 30 individuals,
morphologically identified as C. gigas, were collected from Bandırma
bay. The genomic DNAs were extracted from each sample’s muscle tissue using
universal salt extraction method. Partial sequences of COI and 16S
Mitochondrial DNA loci of the sample DNAs were obtained for species
identification. The sequences were searched against the database and results
were retrieved from BLAST. All the sequences obtained in this study showed
significant similarity with the C. gigas sequences present in the
database (E=0). The sample sequences resulted in 9 different haplotypes for the
COI locus (hd: 0.5296 and variance: 0.01256±0.112) and 5 different haplotypes
for the 16S rDNA locus (hd: 0.2529, Variance: 0.01076±0.104). The results of
this study provided the first molecular evidence for the presence of non-native
Pacific oyster individuals in the Marmara Sea.

Keywords

Crassostrea gigas, Invasive species, COI gene, 16S mtDNA, Marmara Sea

Supporting Institution

This work was supported by the Scientific Research Projects Coordination Unit of Çanakkale Onsekiz Mart University (Project No: FBA 2014-331, granted to Sefa Acarlı).

Project Number

FBA 2014-331

Thanks

This work was supported by the Scientific Research Projects Coordination Unit of Çanakkale Onsekiz Mart University (Project No: FBA 2014-331, granted to Sefa Acarlı).

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