Evaluation of the Relationship Between Cervical Intraepithelial Neoplasia Grades and Connexin 43

Year 2017, Volume: 3 Issue: 3, 15 - 19, 28.12.2017

Havva Erdem , Mürüvvet Akçay Çelik

https://doi.org/10.19127/mbsjohs.332613

Abstract

Objective: Cervical intraepithelial neoplasia (CIN) is a
premalign cervical disease. The microscopic features of CIN indicates an
alteration leading to dedifferentiation and loss of maturation in the squamous
epithelium.

Disruption of connexins are frequently reported
in malignant cell lines. Here, it is aimed to show the relationship between the
dysplasia grades and connexin.

Methods: 79 cases were included in the study who were
referred to the pathology department between 2014 and 2015 and who had CIN
(grade 1, 2, 3). Sections of 3 micrometer thickness were taken from the
paraffin blocks of the uterus on the polylysine slide. Cx43 antibody with ABC tecnique
were performed to these sections. Staining cells were defined as positive. The
cases were graded according to the intensity of the staining.

Results: The distribution of 79 dysplastic cases was as
follows. 41 of these cases had CIN 1. The average age of these women was 44.93.
16 women were diagnosed with CIN2. The average age of these women was 42.06. 22
women were diagnosed with CIN 3. The average age of the women was 48.87.

Conclusion: In this study, complete loss of Cx43 expression
was observed in all dysplastic cervical cases.

Keywords

Cervical intraepithelial neoplasia, degree of dysplasia, Cx43

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