Antimicrobial Activity Of Silver Nanoparticles Synthesized with Extract of Tomato plant Against Bacterial and Fungal Pathogens

Year 2019, Volume: 5 Issue: 2, 67 - 73, 28.08.2019

Mehmet Fırat Baran , Hilal Acay

https://doi.org/10.19127/mbsjohs.551132

Cited By: 13

Abstract

Objective:
Silver nanoparticles (AgNPs) have a wide range of
applications. Environmental-friendly synthesis methods for these nanoparticles
are more preferable due to their various advantages. This study aimed to
synthesize AgNPs using the extract of the tomato plant in an easy and
economical way. and testing this AgNPs against some human pathogens.

Methods:
Silver nanoparticles were synthesized using aqueous
silver nitrate and reducing tomato plant extract. The characterization of AgNPs
was determined by ultraviolet-visible spectrophotometry (UV-Vis), X-ray
crystallography (XRD) Scanning electron microscopy (SEM), Fourier transform
infrared Spectroscopy (FT-IR), energy dispersive X-ray spectrum (EDAX),
thermogravimetric - differential thermal analysis (TGA-DTA) data. The effects
of the particles on pathogenic microorganisms were determined by minimum
inhibition concentration (MIC).

Results:
These data, with a maximum absorbance of 450.51 nm, in
the spherical view, with the peaks and values of 111^o, 200^o,
220^o and 311^o (38.08, 44.28, 64.42 and 77.34), AgNPs
showed a cubic crystal structure and, using the Debye-Scherrer equation, it was
determined that they had a crystal size of 21.11 nm AgNPs had an antimicrobial
activity on hospital pathogens gram negative, gram positive and Candida
albicans yeast.

Conclusion:
We found that these particles showed antimicrobial
activity on various microorganisms even at very high concentrations. As a
solution to the antimicrobial search, it can be developed in medical industry.

Keywords

Antimicrobial activity, XRD, SEM, TGA-DTA, Silver nanoparticle

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