Sıçan Fetusların Karaciğerlerinde Parasetamolün Vascular Endothelial Growth Factor, Sclerostin ve FETUIN-A Üzerine Etkileri

Year 2020, , 150 - 155, 12.07.2020

Seher Yilmaz , Yeşim Göçmen , Adem Tokpınar , İlyas Uçar Şükrü Ateş Seda Avnioğlu , Mehtap Nisari

https://doi.org/10.30565/medalanya.688286

Abstract

Amaç: Parasetamol gebe, çocuk ve yaşlılar gibi önemli gruplar tarafından yaygın olarak kullanılmaktadır. Özellikle gebelikte fazla dozda alınan parasetamol karaciğer yetmezliklerine neden olmaktadır. Çalışmamızın amacı gebelik döneminde yaygın olarak olarak kullanılan parasetamolün fetüs karaciğeri üzerine etkilerini biyokimyasal olarak araştırmaktır.

Yöntemler: Çalışmamızda 10 adet (n=2) gebe sıçanlardan Wistar Albino cinsi rastgele seçilen kontrol grubu, 50 mg/kg parasetamol grubu, 125 mg/kg parasetamol grubu, 250 mg/kg parasetamol grubu ve 500 mg/kg parasetamol grubu olmak üzere beş gruba ayrıldı. Gavaj yoluyla parasetamol, belirlenen dozlarda gebe sıçanlara verildi. Gebeliğin 20. gününde sezeryan ile fetüsler alındı (her gruptan 10 adet fetus alındı). Sonrasında fetus karaciğerleri biyokimysasal analiz için alındı. Biyokimyasal olarak vascular endothelial growth factor A (VEGF-A), FETU-A (α2-heremans-schmid glikoprotein) ve Sclerostin (SOST) değerleri incelendi.

Bulgular: Bu çalışmada parasetamol dozu arttıkça karaciğer hepatosit hücrelerinde değişiklikler görülmektedir. VEGF-A ve FETU-A’da düzenli artış görülmektedir. SOST ise 250 mg/kg dozunda yükselirken, 500 mg/kg parasetamol grubunda azalmıştır. (p<0.05)

Sonuç: Sonuç olarak gebelikte yüksek dozda parasetamol kullanımının fetüskaraciğerinde ve biyokimyasal değerde değişiklik meydana getirdiği görülmektedir. Bu çalışmada gebelikte masum analjezik olarak reçetesiz satılan ve çok kullanılan parasetamol’un yüksek dozda kullanımı konusunda dikkat edilmesi gerektiğini ve bu çalışmanın yapılacak diğer çalışmalara referans olacağını düşünmekteyiz. 

Keywords

VEGF, parasetamol, karaciğer, VEGF-A, Fetüs

Effects of Paracetamol on Vascular Endothelial Growth Factor, Sclerostin and FETUIN-A in the Liver of Rat Fetuses

Abstract

Aim: Paracetamol is widely used by important societal groups such as pregnant women and the elderly. Paracetamol, taken in high doses especially during pregnancy, causes liver failure. The aim of our study is to investigate the effects of paracetamol, which is widely used during pregnancy, on the fetal liver. 

Methods: In our study, five groups of randomly selected rats from 10 Wistar Albino rats (n=2) were formed as control group, 50 mg / kg paracetamol group, 125 mg / kg paracetamol group, 250 mg / kg paracetamol group and 500 mg / kg paracetamol group. Paracetamol by gavage was given to pregnant rats in specified doses. Fetuses were taken by cesarean on the 20th day of pregnancy (10 fetuses were taken from each group). The fetus livers were then taken for biochemical analysis. Biochemically, vascular endothelial growth factor A (VEGF-A), FETU-A (FETUIN-A) (α2-heremans-schmid glikoprotein) and Sclerostin (SOST) values were examined.

Results: In this study, changes in liver hepatocyte cells are seen as the dose of paracetamol increases. Regular increase is observed in VEGF-A and FETU-A. SOST increased at a dose of 250 mg / kg and decreased in the group of 500 mg / kg paracetamol. (p<0.05).

Conclusions: As a result, it is seen that the use of high doses of paracetamol in pregnancy causes changes in the liver and many biochemical values on the fetus. We think that an overdosing of paracetamol, which is sold without prescription and used as an innocent analgesic during pregnancy, should be examined and this study will be a reference for other studies to be conducted.

Keywords

VEGF, paracetamol, liver, VEGF-A, Fetus

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