Antibacterial effects of leukocyte and platelet-rich fibrin against Escherichia coli and Enterococcus faecalis

Year 2021, , 132 - 137, 30.08.2021

Mehtap Bilgin Çetin , Yasemin Sezgin , Ebru Evren , Prof. Dr. Şule Bulut

https://doi.org/10.30565/medalanya.850173

Abstract

Aim: The main goal of this research was to explore the in-vitro antibacterial characteristics of leukocyte and platelet-rich fibrin (L-PRF) against Escherichia coli (E. Coli) and Enterococcus faecalis (E. Faecalis).

Materials and methods: The study was conducted on 21 patients (10 females, 11 males, age range 21-32 years). L-PRF was prepared from the participants’ own blood. Antibacterial activity of L-PRF against E. coli and E. faecalis ATCC standard strains was analyzed using the Kirby Bauer disk diffusion method.

Results: The inhibition zones with PRF had not been detected even though the results obtained using control discs were in accordance with the expectations.

Conclusion: L-PRF demonstrated no inhibition zone against E. coli and E. faecalis.

Keywords

Antibacterial, Escherichia Coli, Enterococcus Faecalis, Leukocyte and Platelet-Rich Fibrin, postoperative infection.

Supporting Institution

Başkent Üniversitesi Diş Hekimliği Fakültesi

Project Number

D-KA 14/21

Thanks

Authors, thank to all individuals who participated in the study.

Lökosit ve Trombositten Zengin Fibrinin Escherichia Coli’ye ve Enterococcus Faecalis'e Karşı Antibakteriyel Etkileri

Abstract

Amaç: Bu çalışmanın amacı, lökosit ve trombositten zengin fibrinin (TZF) Escherichia coli’ye (E. Coli) ve Enterococcus faecalis'e (E. Faecalis) karşı in vitro antibakteriyel etkilerini araştırmaktır.

Gereç ve yöntemler: Çalışma 21 hasta (10 kadın, 11 erkek, yaş aralığı 21-32 yaş) üzerinde yürütülmüştür. Katılımcılardan elde edilen kan örnekleri kullanılarak TZF hazırlanmıştır. TZF'nin E. coli ve E. faecalis ATCC standart suşlarına antibakteriyel aktivitesi Kirby Bauer disk difüzyon yöntemi kullanılarak test edilmiştir.

Bulgular: Beklenen sonuçlar kontrol diski ile elde edilmesine rağmen, TZF’nin değerlendirilen bakterilere karşı inhibisyon zonu göstermediği tespit edilmiştir.

Sonuç: TZF, E.coli’ye ve E. faecalis’e karşı inhibisyon bölgesi göstermemiştir.

Keywords

Antibakteriyel,, Escherichia Coli,, Enterococcus Faecalis, lökosit ve trombositten zengin fibrin, postoperatif enfeksiyon

Project Number

D-KA 14/21

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