Detection of intestinal parasites by different methods in our type 2 diabetic patients

Year 2022, , 64 - 71, 27.03.2022

Müge Özsan Yılmaz

https://doi.org/10.30565/medalanya.987899

Cited By: 1

Abstract

Aim: Long term persistently high blood glucose levels result in various complications and conditions in diabetic patients. One of them is gastrointestinal disorders and the other is increased risk of infectious diseases like parasitosis. The aim of the study is to demonstrate of intestinal parasites with various techniques in diabetic patients and confirm of the frequency of the parasites.

Methods: A total of 65 patients with type 2 Diabetes Mellitus were included in the study. Laboratory tests were done and gastrointestinal symptoms were recorded. Fecal specimens were evaluated with direct microscopy, Kinyoun acid-fast staining method, trichrome staining method and antigen screening test.

Results: Of the patients included in the study 31 were male and 34 were female. While 53.8% of the patients had no chronic complications of diabetes, 33.8% had multiple complications. Thirty (46.2%) patients had gastrointestinal complaints. Examination of stool samples revealed G. intestinalis in two patients (3.07%), C. parvum in three patients (4.6%), and G. intestinalis + E. histolytica in six patients (9.2%) by RAT. No association was found between the existence of parasite determined by RAT and any of the patient characteristics of age, sex, duration of diabetes, and dyspeptic complaints (p-values are 0.27; 0.14; 0.90; 0.68, respectively).

Conclusion: This is the first study to explore the prevalence rate of parasitosis detected by RAT in patients with diabetes. In this study, we also compared different parasite detection methods in this patient population and showed that RAT is a more sensitive method.

Keywords

Type 2 Diabetes Mellitus, Parasitic Intestinal Diseases, Gastrointestinal Disorders, antigen, microscopy

Tip 2 diyabetik hastalarımızda farklı metotlarla intestinal parazitlerin tespiti

Abstract

Amaç: Uzun süreli kalıcı yüksek kan şekeri seviyeleri diyabetik hastalarda çeşitli olumsuz sonuçlara yol açar. Bunlardan bir tanesi gastrointestinal bozukluklar ve bir diğeri de parazitozlar gibi enfeksiyöz hastalıklardaki risk artışıdır. Bu çalışmanın amacı diyabetik hastalarda bağırsak parazitlerinin çeşitli tekniklerle gösterilmesi ve sıklığının belirlenmesidir.

Yöntemler: Çalışmaya 65 tip 2 diyabet hastası dahil edildi. Rutin laboratuvar testleri yapıldı ve semptomları kaydedildi. Fekal örneklerde direkt mikroskopi, Kinyoun asit fast boyama, trikrom boyama, hızlı antijen tarama (HAT) teknikleriyle intestinal parazit arandı.

Bulgular: Çalışmaya dahil edilen hastaların 31’ i erkek, 34’ü kadın cinsiyette idi. Hastaların %53.8'inde diyabetin kronik komplikasyonu mevcut olmayıp, %33.8'inde çoklu komplikasyon mevcuttu. Otuz (%46,2) hastada gastrointestinal şikayetler tespit edildi. Dışkı örneklerinin incelenmesinde HAT ile iki hastada (%3.07) G. intestinalis, üç hastada (%4.6) C. parvum ve altı hastada (%9.2) G. intestinalis + E. histolytica tespit edildi. HAT ile belirlenen parazit varlığı ile yaş, cinsiyet, diyabet süresi ve dispeptik şikayetler gibi hasta özelliklerinden herhangi biri arasında ilişki bulunmadı (p değerleri sırasıyla 0,27; 0,14; 0,90; 0,68'dir).

Sonuç: Bu çalışma diyabetik hastalarda HAT ile parazitoz prevalansını araştıran ilk çalışmadır. Ayrıca bu çalışmada bu hasta popülasyonunda farklı parazit tespit yöntemlerini de karşılaştırdık ve HAT’ın daha sensitif bir yöntem olduğunu gösterdik.

Keywords

Tip 2 Diabetes Mellitus, parazitik bağırsak hastalıkları, gastrointestinal hastalıklar, antijen, mikroskopi

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