Brusellozun Tanısında Yeni Bir Metot Olan Brucella Coombs Gel Testin Diğer Yöntemlerle Karşılaştırılması

Year 2020, Volume: 4 Issue: 1, 56 - 60, 02.03.2020

Murat Karameşe , Osman Acar

https://doi.org/10.30565/medalanya.646672

Cited By: 1

Abstract

Amaç: Bu çalışmada, rutin laboratuvarlarda brusellozun tanısında kullanılan Brucella immuncapture test (BCAP), Rose Bengal (RB), Standart Tüp Aglütinasyon (STA) ve ELISA testleri ile yeni bir serolojik test olan Brucella Coombs Gel Test (BCGT)’in etkinliğinin karşılaştırılması amaçlanmıştır.

Hastalar ve Yöntem: Dâhiliye, enfeksiyon hastalıkları ve pediatri kliniklerinden bruselloz ön tanısı ile laboratuvarımıza gönderilen 107 hastaya ait serum örneklerinde BCAP, RB, STA, ELISA IgG/IgM ve BCGT testleri uygulanmıştır. Cohen’s Kappa testi ile tanı testleri arasındaki uyum istatistiksel olarak analiz edilmiştir. Ayrıca, BCGT testinin duyarlılık, özgüllük, pozitif ve negatif prediktif değerleri saptanmıştır.

Bulgular: Elde edilen verilere göre; 107 hastanın 102’si (95.3%) BCAP testi ile 96’sı (%89.7) RB testi ile 80’i (%74.8) STA testi ile 100’ü (%93.5) BCGT ile 104’ü (%97.2) ELISA IgG testi ile ve 101’i (94.3%) ELISA IgM testi ile pozitif olarak tespit edilmiştir. Yapılan istatistiksel analizler sonucunda (Cohen’s Kappa Test), BCAP ile BCGT (κ=0.824) arasında güçlü uyum saptanmıştır. Ayrıca BCGT testinin duyarlılık ve özgüllüğü sırasıyla %98.08 ve %71.43 olarak saptanmıştır.

Sonuç: BCGT insan brusellozunun tanısında kullanılabilecek umut vadeden bir teknik gibi görünen, hızlı, az maliyetli ve yüksek duyarlılığa sahip bir testtir. Ancak, bu testin rutin laboratuvarlarında kullanılabilirliğini destekleyecek daha fazla bilimsel çalışmaya ihtiyaç vardır.

Keywords

Brusella, Rose Bengal, Coombs jel test, Standart tüp aglütinasyon

Supporting Institution

Kafkas Üniversitesi BAP Koordinatörlüğü

Project Number

2015-TS-16

Thanks

Kafkas Üniversitesi BAP Koordinatörlüğü'ne ve Zakir Zeki ÇALIK'a desteklerinden ötürü teşekkür ederiz.

A comparison of a new diagnostic test of the human Brucellosis, the Brucella Coombs Gel Test, with other methods

Abstract

Aim: The aim of this study was to compare the efficiency of the Brucella Coombs Gel Test (BCGT), a new serological diagnosis test, with the methods used in routine laboratory such as Brucella immuncapture test (BCAP), Standard Tube Agglutination (STA), Rose Bengal (RB) and ELISA for the diagnosis of brucellosis.

Patients and Method: The serum samples of 107 patients with a presumptive diagnosis of brucellosis sent from three different clinics (internal medicine, infectious disease and pediatric clinics) were subjected to four different diagnostic methods (BCAP, RB, STA, and ELISA). The correlations between these diagnostic tests were analyzed using the Cohen’s Kappa test. Additionally, sensitivity, specificity, positive and negative predictive values, and accuracy of BCGT were measured.

Results: According to the obtained data, the positivity of different Brucella tests (BCAP, RB, STA, BCGT, ELISA IgG and IgM) were 102 (95.3%), 96 (89.7%), 80 (74.8%), 100 (93.5%), 104 (97.2%) and 101 (94.3%), respectively. According to the Kappa test results, there was strong agreement between BCAP and BCGT (κ=0.824). Furthermore, the sensitivity and specificity values of BCGT in our study were 98.08% and 71.43%, respectively.

Conclusion: BCGT is a rapid, cost-effective and highly sensitive test, which appears to be a promising technique for the diagnosis of human brucellosis; however, further scientific studies are needed to support the applicability of this test in routine laboratories.

Keywords

Brucella, Rose Bengal, Coombs gel test, Standart tube agglutination

Project Number

2015-TS-16

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