The protective role of ferulic acid against imidacloprid-induced oxidative stress in liver and brain of Cyprinus carpio

Year 2023, Volume: 6 Issue: 2, 49 - 59, 31.12.2023

Ferbal Özkan Yılmaz , Arzu Özlüer Hunt , Önder Şahin , Metin Yıldırım , Mehmet Berköz

Abstract

This study evaluated the potential protective effect of ferulic acid (FA) on stress induced by imidacloprid (IM) in Cyprinus carpio (mean weight 60.10±2.22 g). Therefore, C. carpio was exposed to sublethal IM con-centration (2.80 mg/L) for 10 days. During the experimental period, fish were fed a basal (control) diet and a basal diet supplemented with 5 g/kg of ferulic acid. The fish were divided into four groups (n = 6 in each group). The control group was exposed to tap water (the absence of imidacloprid) fed with control diet. The FA group was exposed to tap water (the absence of IM) and was fed with FA supplemented diet. The liver and brain tissues of both control and treated fish were dissected. Tissues samples were obtained from an in-dividual fish and prepared for analysis. The variations in catalase (CAT), superoxide dismutase (SOD) activi-ties, and levels of malondialdehyde (MDA) with protein carbonyl (PCO) in liver and brain tissues of C. car-pio were investigated in experimental groups. CAT activities were significantly increased whereas SOD activi-ties were decreased in liver and brain tissues of treated fish by exposure to imidacloprid. Tissue MDA and PCO levels in the IM supplemented experimental groups were increased compared to the control. At the end of the experiment, it was determined that FA application had the effect of improving tissue enzyme activities and levels of MDA and PCO.

Keywords

Cyprinus carpio, imidacloprid, ferulic acid, antioxidant system, protein carbonyl

References

• Ademuyiwa, O., Agarwal, R., Chandra, R., Behari, J.R. (2009) Lead induced phospholipidosis and cholesterogenesis in rat tissue. Chemico-Biological Interactions 179(2-3): 314-320.
• Aebi, H. (1974) Catalase, in: Bergmeyer U, (Ed.), Methods of Enzymatic Analysis. New York and London, pp. 673-677.
• Ahmadifar, E., Moghadam, M.S., Dawood, M.A.O., Hoseinifar, S.H. (2019) Lactobacillus fermentum and/or ferulic acid improved the immune responses, antioxidative defence and resistance against Aeromonas hydrophila in common carp (Cyprinus carpio) fingerlings. Fish & Shellfish Immunology 94: 916-923
• Awoyemi, O.M., Bawa-Allah, K.A., Otitoloju, A.A. (2014) Accumulation and anti-oxidant e zymes as biomarkers of heavy metal exposure in Clarias gariepinus and Oreochromis niloticus. Applied Ecology and Environmental Sciences 2(5): 114-122.
• Berlett, B.S., Stadtman, E.R. (1997) Protein oxidation in aging, disease, and oxidative stress. The Journal of Biological Chemistry 272(33): 20313-20316.
• Berrahal, A.A., Nehdi, A., Hajjaji. N., Gharbi. N., El-Fazâa., S. (2007) Antioxidant enzymes activities and bilirubin level in adult rat treated with lead. Comptes Rendus Biologies 330: 581-588.
• Bourne, L.C., Rice-Evans, C., (1998) Bioavailability of ferulic acid. Biochemical and Biophysical Research Communications 25: 222-227.
• Boyd, C.E., D'Abramo, L.R., Glencross, B.D., Huyben, D.C., Juarez, L.M., Lockwood, G.S., McNevin, A.A., Tacon, A.G.J., Teletchea, F., Tomasso, J. R., Jr., Tucker, C.S., Valenti, W.C. (2020) Achieving sustainable aquaculture: Historical and current perspectives and future needs and challenges. Journal of the World Aquaculture Society, 51: 578-633.
• Davies, M. (2016) Protein oxidation and peroxidation. Biochemical Journal 473(7): 805-825.
• Dawood, M.A.O., Metwally, A.E., El-Sharawy, M.E., Ghozlan, A.M., Abdel-Latif, H.M.R., Doan, H.V., Mervat, A.M.A. (2020) The influences of ferulic acid on the growth performance haemato-immunological responses, and immune-related genes of Nile tilapia (Oreochromis niloticus) exposed to heat stress. Aquaculture 525: 735320.
• Desai, B., Parikh. P. (2013) Biochemical alterations on exposure of imidacloprid and curzate on fresh water fish Oreochromis mossambicus and Labeo rohita. Indian Journal of Forensic Medicine and Toxicology 7(2): 53-59.
• El-Gendy, K.S., Aly, N.M., Mahmoud, F.H., Kenawy, A., El-Sebae, A.H. (2010) The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid. Food and Chemical Toxicology 48: 215-221.
• El-Shenawy, N.S., El-Salmy, F., Al-Eisa, R.A., El-Ahmary, B. (2010) Amelioratory effect of vitamin E on organophosphorus insecticide diazinon-induced oxidative stress in mice liver. Pesticide Biochemistry and Physiology 96: 101-7.
• Ge, W., Yan, S., Wang, J., Zhu, L., Chen, A., Wang. J. (2015) Oxidative stress and DNA damage induced by imidacloprid in zebrafish (Danio rerio). Journal of Agricultural and Food Chemistry 63: 1856-1862.
• Ghosh, S., Basak, P., Dutta, S., Chowdhury, S., Sil, P.C. (2017) New insights into the ameliorative effects of ferulic acid in pathophysiological conditions. Food and Chemical Toxicology 103: 41-55
• Gonzalez, P.M., Malanga, G., Puntarulo, S. (2015) Cellular oxidant/antioxidant network: Update on the environmental effects over marine organisms. The Open Marine Biology Journal, 9: 1-13.
• Gupta, R.C. (2004) Brain regional heterogeneity and toxicological mechanisms of organophosphates and carbamates. Toxicology Mechanisms and Methods 14: 103-143.
• Jablonska-Trypuc, A. (2017) Pesticides as inducers of oxidative stress. Reactive Oxygen Species 3(8): 96-110.
• Kehm, R., Baldensperger, T., Raupbach, J., Hohn, A. (2021) Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases. Redox Biology 42: 101901
• Levine, R.L., Williams, J.A., Stadtman, E.R., Shacter, E. (1994) Carbonyl assays for determination of oxidatively modified proteins. Methods in Enzymology, 233: 346-357.
• Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall. R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193: 265-275.
• Maurya, D.K., Devasagayam, T.P.A. (2010) Antioxidant and prooxidant nature of hydroxy cinnamic acid derivatives ferulic and caffeic acids. Food Chemical Toxicology 48: 3369-3373.
• Mehta, A., Verma, R.S., Srivastava, N. (2009). Chlorpyrifos induced alterations in the levels of hydrogen peroxide, nitrate and nitrite in rat brain and liver. Pesticide Biochemistry and Physiology 94: 55-59.
• Mohan, K., Rajan, D.K., Muralisankar, T., Ganesan, A.R., Marimuthu, K., Sathishkumar, P. (2022). The potential role of medicinal mushrooms as prebiotics in aquaculture: A review. Reviews in Aquaculture 14: 1300-1332.
• Pandey, S., Parvez, S., Sayeed, I., Haque, R., Bin-Hafeez, B., Raisuddin, S. (2003) Biomarkers of oxidative stress: a comparative study of River Yamuna fish Wallago attu (Bl. and Schn.). Science of The Total Environment 309: 105-115.
• Parvez, S., Raisuddin, S. (2005) Protein carbonyls: novel biomarkers ofexposure to oxidative stress-inducing pesticides in freshwater fish Channa punctata (Bloch). Environmental Toxicology and Pharmacology 20: 112–117.
• Repetto, M., Semprine, J., Boveris, A. (2012) Lipid peroxidation: chemical mechanism, biological implications and analytical determination. In: Catala, A., (Ed.), Lipid peroxidation, New Delhi, Intech, 1-28.
• Rice-Evans, C.A., Miller, N.J., Bolwell, P.G., Bramley, P.M., Pridham, J.B. (1995) The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Research 22: 375-383.
• Santos, L., Ramos, F. (2018) Antimicrobial resistance in aquaculture: Current knowledge and alternatives to tackle the problem. International Journal of Antimicrobial Agents 52: 135-143.
• Shao, B., Zhu, L.S., Dong, M., Wang, J., Wang, J.H., Xie, H., Zhang, Q.M., Du, Z.Q., Zhu, S.Y. (2012). DNA damage and oxidative stress induced by endosulfan exposure in zebra fish (Danio rerio). Ecotoxicology 21: 1533-1540.
• Shukla, S., Jhamtani, R.C., Dahiya, M.S., Agarwal, R. (2017) Oxidative injury caused by individual and combined exposure of neonicotinoid, organophosphate and herbicide in zebrafish. Toxicology Reports 4: 240–244.
• Stephan, G., Guillaumeb, J., Lamoura, F. (1995). Lipid peroxidation in turbot (Scophthahus maximus) tissue: effect of dietary vitamin E and dietary n-6 or n-3 polyunsaturated fatty acids. Aquaculture 130: 251–268
• Sun, Y., Oberley, L.W., Ying, L.A. (1988) Simple method for clinical assay of superoxide dismutase. Clinical Chemistry 34: 497-500.
• Topal, A., Alak, G., Ozkaraca, M., Cilingir-Yeltekin, A., Comaklı, S., Acıl, G., Kokturk, M., Atamanalp, M. (2017) Neurotoxic responses in brain tissues of rainbow trout exposed to imidacloprid pesticide: Assessment of 8-hydroxy-2-deoxyguanosine activity, oxidative stress and acetylcholinesterase activity, Chemosphere 175: 186-191.
• Trenzado, C., Hidalgo, M.C., Garcia-Gallego, M., Morales, A.E., Furne, M., Domezain, A., Domezain, J., Sanz, A. (2006). Antioxidant enzymes and lipid peroxidation in sturgeon Acipenser naccari and trout Oncorhynchus mykiss. A comparative study. Aquaculture 254: 758-767.
• Tyor, A.K, Harkrishan K. (2016) Effects of imidacloprid on viability and hatchability of embryos of the common carp (Cyprinus carpio L.). International Journal of Fisheries and Aquatic Studies 4(4): 385-389.
• Vieira, C.E.D., Perez, M.R., Acayaba, R.D., Raimundo, C.C.M., Dos Reis Martinez, C.B. (2018) DNA damage and oxidative stress induced by imidacloprid exposure in different tissues of the Neotropical fish Prochilodus lineatus. Chemosphere, 195: 125-134.
• Vinodhini, R., Narayanan, M. (2008). Bioaccumulation of heavy metals in organs of fresh water fish Cyprinus carpio (Common carp). International Journal of Environmental Science and Technology 5(2): 179-182.
• Vlahogianni, T., Dassenakis, M., Scoullos, M.J., Valavanidis, A. (2007) Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in mussels Mytilus galloprovincialis for assessing heavy metals’ pollution in coastal areas from the Saronikos Gulf of Greece. Marine Pollution Bulletin 54: 1361-1371.
• Yagi, K. (1998) Simple procedure for specific enzyme of lipid hydroperoxides in serum or plasma. Methods in Moleculer Biology 108: 107-110.
• Yu, L., Wu, F., Liu, W., Tian, J., Lu, X., Wen, H. (2017) Semisynthetic ferulic acid derivative: An efficient feed additive for Genetically Improved Farmed Tilapia (Oreochromis niloticus). Aquaculture Research 48: 5017-5028.