Sisplatin’e Maruz Bırakılan Sıçan Böbreğinde Lutein’in Koruyucu Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi

Year 2022, Volume: 4 Issue: 3, 433 - 438, 22.09.2022

Sedat Bilgiç , Fatih Mehmet Gür , İbrahim Aktaş

https://doi.org/10.37990/medr.1142424

Cited By: 3

Abstract

Amaç: Çalışmamızda, sisplatin (Cis) (10mg/kg, intraperitoneal) kaynaklı böbrek hasarına karşı lutein’in (L) (100mg/kg, oral) koruyucu etkisi incelendi.
Materyal ve Metot: Bu çalışma, 8 haftalık 28 adet sıçanla gerçekleştirildi. Oluşturulan 4 grubun her birinde 7 tane sıçan yer aldı: Kontrol, L, Cis ve Cis + L.
Bulgular: Biyokimyasal bulgulara göre, Cis grubunun kontrol grubu ile kıyaslanmasında serum Cr ve BUN seviyelerinin anlamlı düzeyde arttığı görüldü (p<0.05). Cis + L grubunda serum Cr ve BUN düzeylerinin Cis grubuna kıyasla anlamlı düzeyde azaldığı tespit edildi (p<0.05). Ayrıca Cis + L grubunun Cis grubu ile kıyaslanmasında MDA seviyesinde düşüş, GSH, SOD ve CAT seviyelerinde ise artış kaydedildi (p<0.05). Yapılan histopatolojik incelemelerde Cis grubu sıçanların böbrek dokularında interstisyel mononükleer lökosit infiltrasyonu, tubuler dejenerasyon ve tubuler nekroz gibi patolojik değişiklikler gözlendi. Cis + L grubu Cis grubu ile kıyaslandığında L’nin etkisi ile oksidatif streste bir azalma, antioksidan aktivitede bir artma ve histopatolojik değişikliklerde bir azalma olduğu söylenebilir.
Sonuç: Bu sonuçlar L’nin Cis kaynaklı böbrek hasarının önlenmesinde etkili olduğunu göstermektedir. Ayrıca L’nin bu hasarda kullanılma potansiyeli olan bir farmakolojik ajan olduğuda ortaya çıkmaktadır.

Keywords

Antioksidan, böbrek, lutein, sisplatin, sıçan

Supporting Institution

Projemizi fonlayan herhangi bir kurum bulunmamaktadır.

Project Number

2022/036

Biochemical and Histopathological Investigation of the Protective Effect of Lutein in Rat Kidney Exposed to Cisplatin

Abstract

Aim: The aim of this study was to determine the protection of lutein (L) (100 mg/kg, orally) against kidney damage caused by cisplatin (Cis) (10mg/kg, intraperitoneal).
Materials and Methods: Twenty-eight rats aged 8 weeks were used and divided into four equal groups (n=7): Control, L, Cis, and Cis + L.
Results: As a result of biochemical findings, serum Cr and BUN levels of the Cis group were significantly increased compared to the control group (p<0.05). Serum Cr and BUN levels of the Cis + L group were found to be significantly reduced compared to the Cis group (p<0.05). In addition, a decrease in MDA level and an increase in GSH, SOD and CAT levels were detected in the Cis + L group compared to the Cis group (p<0.05). In the histopathological examinations, in the Cis group kidney tissues; pathological changes such as interstitial mononuclear leukocyte infiltration, tubular degeneration, and tubular necrosis were observed. When the Cis + L group is compared with the Cis group, it can be said that there is a decrease in oxidative stress, an increase in antioxidant activity and a decrease in histopathological changes with the effect of L.
Conclusion: These results show that L is effective in preventing Cis-induced kidney injury. It is also emerging that L is a pharmacological agent with the potential to be used in this damage.

Keywords

Antioxidant, kidney, lutein, cisplatin, rat

Project Number

2022/036

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