THE PREPARATION OF A NEW PROTEIN BASED HPLC COLUMN PACKING MATERIAL AND ENANTIOMERIC RESOLUTION OF SOME PHARMACEUTICAL RELATED COMPOUNDS VIA THIS COLUMN

Year 2016, Volume: 2 Issue: 1, 77 - 92, 29.06.2016

Ömer Erdoğan , Giray Topal , Reşit Çakmak , Murat Sünkür , Mutlu Canpolat

https://doi.org/10.23884/mejs/2016.2.1.07

Abstract

In this study, BSA was bounded to 3-chloropropyl functionalized silica gel via ethylenediamine and glutaric dialdehyde. The prepared CSP was packed into 150X4.6mm HPLC column according to slurry method. The enantiomeric resolution of some pharmaceutical related compounds such as rac-mandelic acid, rac-methyl mandelate, rac-epinephrine, rac-propranolol, rac-α-phenylethylamine and rac-3-hydroxybutyric acid was employed in 1mL/rpm, 1,5mL/rpm, 2mL/rpm, 2,5mL/rpm, 3mL/rpm flow rate in various pHs such pH=6, pH=7, pH=8 0,1M phosphate buffer. Chromatographic parameters such as Rs and α were calculated for each resoluted compounds.

Keywords

Enantiomeric separation, hplc, bovine serum albumin

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