Research Article
BibTex RIS Cite

Pelargonium quercetorum Agnew. bitkisinin antioksidan aktivitesinin belirlenmesi

Year 2018, Volume: 11 Issue: 2, 174 - 183, 15.08.2018
https://doi.org/10.26559/mersinsbd.398926

Abstract

Amaç: Antiparaziter olarak geleneksel kullanımı
mevcut olan Geraniaceae familyasına dahil Pelargonium
quercetorum
Agnew.’in toplam fenolik madde miktarı ve antioksidan
aktivitesinin araştırılması amaçlanmıştır.
Yöntem:
P. quercetorum'un toprak
altı kısımlarından hazırlanan %70 metanol ve %11 etanol ekstreleri ile toprak
üstü kısmından hazırlanan %70 metanol ekstresinin toplam fenolik, flavonoit ve
flavonol içerikleri spektrofotometrik olarak incelenmiştir. Ekstrelerin
antioksidan aktivitelerini belirlemek amacıyla 1.1-difenil-2-pikrilhidrazil
(DPPH
l) ve 2.2’-azino-bis (3-etilbenzotiazolin-6-
sulfonik asit) (ABTS+
l)
radikal süpürücü etkileri, Fe(III)’ü Fe(II)’ye
indirgeme özellikleri ve demir(II) şelat (kelat) aktiviteleri
incelenmiştir. Bulgular ve Sonuç: %70
Metanol kök ekstresinin toplam fenolik ve flavonoit (242.29 ± 5.52 mgGAE/gekstre,
64.95 ± 2.93mgCA/gekstre) içerik bakımından
zengin olduğu ve antioksidan aktivite deneylerinde de güçlü etki gösterdiği
belirlenmiştir.

References

  • 1. Matkowski A, Zielinska S, Oszmianski J, Lamer-Zarawska E. Antioxidant activity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim., and S. verticillata L. Bioresour Technol 2008; 99: 7892–7896.
  • 2. Larson RA. Naturally occuring antioxidants. Lewis Publishers, New york 1997; pp 1-15.
  • 3. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994; 344:721–724.
  • 4. Pincemail JJ. Free radicals and antioxidants in human diseases. In: Favier AE, Cadet J, Kalyanaraman B, Fontecave M, Pierre JL. (eds.), Analysis of Free Radicals in Biological Systems. Birkhauser Verlag, Berlin 1995, pp 83–98.
  • 5. Dreher D, Junod AF. Role of oxygen free radicals in cancer development. Eur. J. Cancer 1996; 32A: 30–38.
  • 6. Oberley TD, Oberley LW. Antioxidant enzyme levels in cancer. Histol. Histopathol 1997; 12: 525–535.
  • 7. Maxwell S. Anti-oxidant therapy: does it have a role in the treatment of human disease? Exp Opin Invest Drugs 1997; 6: 211–236.
  • 8. Brendlera T, Van Wyk BE. A historical, scientific and commercial perspective on the medicinal use of Pelargonium sidoides (Geraniaceae). J Ethnopharmacol 2008; 119: 420–433.
  • 9. Trun W, Kiderlen AF, Kolodziej H. Nitric oxide synthase and cytokines gene expression analyses in Leishmania-infected RAW 264.7 cells treated with an extract of Pelargonium sidoides (Eps 7630). Phytomed 2006; 13: 570–575. 10. Davis PH, Mill RR, Kit T. Pelargonium L’Hérit In: Flora of Turkey and the East Aegean Islands, Davis PH (eds), University Press, Edinburgh, 1988; 10: pp 106
  • 11. Uce İ, Tunçtürk M. Hakkâri’de Doğal Olarak Yetişen ve Yaygın Olarak Kullanılan Bazı Yabani Bitkiler. Biyoloji Bilimleri Araştırma Dergisi 2014; 7 (2): 21-25.
  • 12. Taherpour AA, Maroofı H, Kheradmand K. Chemical composition of the essential oil of Pelargonium quercetorum Agnew. of Iran. Nat Prod Res 2007; 21(1): 24–27.
  • 13. Kolodziej H, Kiderlen AF. In vitro evaluation of antibacterial and immunomodulatory activities of Pelargonium reniforme, Pelargonium sidoides and the related herbal drug preparation EPs® 7630. Phytomedicine 2007; 14 (6): 18-26.
  • 14. Singleton VL, Orthofer R, Lamuela-Ravento´S RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. In Methods in Enzymology, Packer L (ed.), Academic Press, San Diego, CA, 1999; 299: 152-315.
  • 15. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64: 555−559.
  • 16. Miliauskas G, Venskutonis PR, Van-Beek TA. Screening of Radical Scavenging Activity of Some Medicinal and Aromatic Plant Extracts. Food Chem 2004; 85: 231-237.
  • 17. Oyaizu M. Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986; 44: 307-315.
  • 18. Gyamfi MA, Yonamine M, Aniya Y. Free-Radical Scavenging Action of Medicinal Herbs from Ghana Thonningia Sanguinea on Experimentally Induced Liver Injuries. Gen Pharmacol 1999; 32: 661-667.
  • 19. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Bio Med 1999; 26: 1231-1237.
  • 20. Carter P. Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Anal Biochem 1971; 40: 450–458.
  • 21. Çakal S. Pelargonium endlicherianum Fenzl.(Geraniaceae) kök ekstrelerinin UMCA® preparatı ile karşılaştırılmalı araştırılması, Yüksek Lisans Tezi, Anadolu Üniversitesi, Sağlık Bilimleri Enstitüsü, Eskişehir 2009, ss 15-27.
  • 22. Tepe B, Sökmen M, Akpulat HA, Yumrutaş Ö, Sökmen A. Screening of antioxidative properties of the methanolic extracts of Pelargonium endlicherianum Fenzl. Verbascum wiedemannianum Fisch. & Mey. Sideritis libanotica Labill. subsp. linearis (Bentham) Borm. Centaurea mucronifera DC. and Hieracium cappadocicum Freyn. from Turkish flora. Food Chem 2006; 98: 9–13.
  • 23. Lalli YY, Van Zyl RL, Van Vuuren SF, Viljoen AM. In vitro biological activities of South African Pelargonium (Geraniaceae) species. S Afr J Bot 2008; 74: 153–157.
  • 24. Arı F, Çelikler S, Karakaş D, Cevatemre B, Ulukaya E. Total Phenolic Content, Antioxidant and Cyto/Genotoxic Activities of Pelargonium quercetorum Agnew in Human Breast Cancer Cells. Journal of Clinical and Experimental Investigations 2017; 8: 22-30.
  • 25. Adewusi EA, Afolayan AJ. Antibacterial, antifungal and antioxidant activity of the roots and leaves of Pelargonium reniforme Curtis (Geraniaceae). Afr J Biotechnol 2009; 8 (22): 6425-6433.
  • 26. Walia H, Kumar S, Arora S. Effect of fractionation on in vitro antiradical efficacy of acetone extract of Terminalia chebula. Afr J Pharm Pharmacol 2014; 8(11): 311-320.
  • 27. Shahidi F. Natural Antioxidants: Chemistry, Health Effects, and Applications, AOCS Press, United States of America 1997; pp 98.
Year 2018, Volume: 11 Issue: 2, 174 - 183, 15.08.2018
https://doi.org/10.26559/mersinsbd.398926

Abstract

References

  • 1. Matkowski A, Zielinska S, Oszmianski J, Lamer-Zarawska E. Antioxidant activity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim., and S. verticillata L. Bioresour Technol 2008; 99: 7892–7896.
  • 2. Larson RA. Naturally occuring antioxidants. Lewis Publishers, New york 1997; pp 1-15.
  • 3. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994; 344:721–724.
  • 4. Pincemail JJ. Free radicals and antioxidants in human diseases. In: Favier AE, Cadet J, Kalyanaraman B, Fontecave M, Pierre JL. (eds.), Analysis of Free Radicals in Biological Systems. Birkhauser Verlag, Berlin 1995, pp 83–98.
  • 5. Dreher D, Junod AF. Role of oxygen free radicals in cancer development. Eur. J. Cancer 1996; 32A: 30–38.
  • 6. Oberley TD, Oberley LW. Antioxidant enzyme levels in cancer. Histol. Histopathol 1997; 12: 525–535.
  • 7. Maxwell S. Anti-oxidant therapy: does it have a role in the treatment of human disease? Exp Opin Invest Drugs 1997; 6: 211–236.
  • 8. Brendlera T, Van Wyk BE. A historical, scientific and commercial perspective on the medicinal use of Pelargonium sidoides (Geraniaceae). J Ethnopharmacol 2008; 119: 420–433.
  • 9. Trun W, Kiderlen AF, Kolodziej H. Nitric oxide synthase and cytokines gene expression analyses in Leishmania-infected RAW 264.7 cells treated with an extract of Pelargonium sidoides (Eps 7630). Phytomed 2006; 13: 570–575. 10. Davis PH, Mill RR, Kit T. Pelargonium L’Hérit In: Flora of Turkey and the East Aegean Islands, Davis PH (eds), University Press, Edinburgh, 1988; 10: pp 106
  • 11. Uce İ, Tunçtürk M. Hakkâri’de Doğal Olarak Yetişen ve Yaygın Olarak Kullanılan Bazı Yabani Bitkiler. Biyoloji Bilimleri Araştırma Dergisi 2014; 7 (2): 21-25.
  • 12. Taherpour AA, Maroofı H, Kheradmand K. Chemical composition of the essential oil of Pelargonium quercetorum Agnew. of Iran. Nat Prod Res 2007; 21(1): 24–27.
  • 13. Kolodziej H, Kiderlen AF. In vitro evaluation of antibacterial and immunomodulatory activities of Pelargonium reniforme, Pelargonium sidoides and the related herbal drug preparation EPs® 7630. Phytomedicine 2007; 14 (6): 18-26.
  • 14. Singleton VL, Orthofer R, Lamuela-Ravento´S RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. In Methods in Enzymology, Packer L (ed.), Academic Press, San Diego, CA, 1999; 299: 152-315.
  • 15. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64: 555−559.
  • 16. Miliauskas G, Venskutonis PR, Van-Beek TA. Screening of Radical Scavenging Activity of Some Medicinal and Aromatic Plant Extracts. Food Chem 2004; 85: 231-237.
  • 17. Oyaizu M. Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986; 44: 307-315.
  • 18. Gyamfi MA, Yonamine M, Aniya Y. Free-Radical Scavenging Action of Medicinal Herbs from Ghana Thonningia Sanguinea on Experimentally Induced Liver Injuries. Gen Pharmacol 1999; 32: 661-667.
  • 19. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Bio Med 1999; 26: 1231-1237.
  • 20. Carter P. Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Anal Biochem 1971; 40: 450–458.
  • 21. Çakal S. Pelargonium endlicherianum Fenzl.(Geraniaceae) kök ekstrelerinin UMCA® preparatı ile karşılaştırılmalı araştırılması, Yüksek Lisans Tezi, Anadolu Üniversitesi, Sağlık Bilimleri Enstitüsü, Eskişehir 2009, ss 15-27.
  • 22. Tepe B, Sökmen M, Akpulat HA, Yumrutaş Ö, Sökmen A. Screening of antioxidative properties of the methanolic extracts of Pelargonium endlicherianum Fenzl. Verbascum wiedemannianum Fisch. & Mey. Sideritis libanotica Labill. subsp. linearis (Bentham) Borm. Centaurea mucronifera DC. and Hieracium cappadocicum Freyn. from Turkish flora. Food Chem 2006; 98: 9–13.
  • 23. Lalli YY, Van Zyl RL, Van Vuuren SF, Viljoen AM. In vitro biological activities of South African Pelargonium (Geraniaceae) species. S Afr J Bot 2008; 74: 153–157.
  • 24. Arı F, Çelikler S, Karakaş D, Cevatemre B, Ulukaya E. Total Phenolic Content, Antioxidant and Cyto/Genotoxic Activities of Pelargonium quercetorum Agnew in Human Breast Cancer Cells. Journal of Clinical and Experimental Investigations 2017; 8: 22-30.
  • 25. Adewusi EA, Afolayan AJ. Antibacterial, antifungal and antioxidant activity of the roots and leaves of Pelargonium reniforme Curtis (Geraniaceae). Afr J Biotechnol 2009; 8 (22): 6425-6433.
  • 26. Walia H, Kumar S, Arora S. Effect of fractionation on in vitro antiradical efficacy of acetone extract of Terminalia chebula. Afr J Pharm Pharmacol 2014; 8(11): 311-320.
  • 27. Shahidi F. Natural Antioxidants: Chemistry, Health Effects, and Applications, AOCS Press, United States of America 1997; pp 98.
There are 26 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Articles
Authors

Gökçe Şeker Karatoprak 0000-0001-5829-6914

Mehmet Fırat This is me 0000-0001-5814-614X

Müberra Koşar This is me 0000-0002-1559-998X

Publication Date August 15, 2018
Submission Date February 26, 2018
Acceptance Date May 3, 2018
Published in Issue Year 2018 Volume: 11 Issue: 2

Cite

APA Şeker Karatoprak, G., Fırat, M., & Koşar, M. (2018). Pelargonium quercetorum Agnew. bitkisinin antioksidan aktivitesinin belirlenmesi. Mersin Üniversitesi Sağlık Bilimleri Dergisi, 11(2), 174-183. https://doi.org/10.26559/mersinsbd.398926
AMA Şeker Karatoprak G, Fırat M, Koşar M. Pelargonium quercetorum Agnew. bitkisinin antioksidan aktivitesinin belirlenmesi. Mersin Univ Saglık Bilim derg. August 2018;11(2):174-183. doi:10.26559/mersinsbd.398926
Chicago Şeker Karatoprak, Gökçe, Mehmet Fırat, and Müberra Koşar. “Pelargonium Quercetorum Agnew. Bitkisinin Antioksidan Aktivitesinin Belirlenmesi”. Mersin Üniversitesi Sağlık Bilimleri Dergisi 11, no. 2 (August 2018): 174-83. https://doi.org/10.26559/mersinsbd.398926.
EndNote Şeker Karatoprak G, Fırat M, Koşar M (August 1, 2018) Pelargonium quercetorum Agnew. bitkisinin antioksidan aktivitesinin belirlenmesi. Mersin Üniversitesi Sağlık Bilimleri Dergisi 11 2 174–183.
IEEE G. Şeker Karatoprak, M. Fırat, and M. Koşar, “Pelargonium quercetorum Agnew. bitkisinin antioksidan aktivitesinin belirlenmesi”, Mersin Univ Saglık Bilim derg, vol. 11, no. 2, pp. 174–183, 2018, doi: 10.26559/mersinsbd.398926.
ISNAD Şeker Karatoprak, Gökçe et al. “Pelargonium Quercetorum Agnew. Bitkisinin Antioksidan Aktivitesinin Belirlenmesi”. Mersin Üniversitesi Sağlık Bilimleri Dergisi 11/2 (August 2018), 174-183. https://doi.org/10.26559/mersinsbd.398926.
JAMA Şeker Karatoprak G, Fırat M, Koşar M. Pelargonium quercetorum Agnew. bitkisinin antioksidan aktivitesinin belirlenmesi. Mersin Univ Saglık Bilim derg. 2018;11:174–183.
MLA Şeker Karatoprak, Gökçe et al. “Pelargonium Quercetorum Agnew. Bitkisinin Antioksidan Aktivitesinin Belirlenmesi”. Mersin Üniversitesi Sağlık Bilimleri Dergisi, vol. 11, no. 2, 2018, pp. 174-83, doi:10.26559/mersinsbd.398926.
Vancouver Şeker Karatoprak G, Fırat M, Koşar M. Pelargonium quercetorum Agnew. bitkisinin antioksidan aktivitesinin belirlenmesi. Mersin Univ Saglık Bilim derg. 2018;11(2):174-83.

MEU Journal of Health Sciences Assoc was began to the publishing process in 2008 under the supervision of Assoc. Prof. Gönül Aslan, Editor-in-Chief, and affiliated to Mersin University Institute of Health Sciences. In March 2015, Prof. Dr. Caferi Tayyar Şaşmaz undertook the Editor-in Chief position and since then he has been in charge.

Publishing in three issues per year (April - August - December), it is a multisectoral refereed scientific journal. In addition to research articles, scientific articles such as reviews, case reports and letters to the editor are published in the journal. Our journal, which has been published via e-mail since its inception, has been published both online and in print. Following the Participation Agreement signed with TÜBİTAK-ULAKBİM Dergi Park in April 2015, it has started to accept and evaluate online publications.

Mersin University Journal of Health Sciences have been indexed by Turkey Citation Index since November 16, 2011.

Mersin University Journal of Health Sciences have been indexed by ULAKBIM Medical Database from the first issue of 2016.

Mersin University Journal of Health Sciences have been indexed by DOAJ since October 02, 2019.

Article Publishing Charge Policy: Our journal has adopted an open access policy and there is no fee for article application, evaluation, and publication in our journal. All the articles published in our journal can be accessed from the Archive free of charge.

154561545815459

Creative Commons Lisansı
This work is licensed with Attribution-NonCommercial 4.0 International.