Abstract
Amaç: Kronik arteryel iskemi tedavisinde cerrahi ve invazif girişimler
yapılmaktayken bu yöntemlerin yetersiz kaldığı durumlarda tedavi
seçeneklerinden biri terapötik anjiyogenezistir. Çalışmamızda, terapötik
anjiyogeneziste yeni ve kontrollü bir süreç oluşturmak amacıyla, arka
bacaklarında kronik arteryel iskemi oluşturulan ratlara, intramusküler
lipoteikoik asit enjeksiyonu yapıldı ve anjiyogenezis süreci değerlendirildi. Yöntem: Gruplardaki rat sayısı istatistiksel yöntemle belirlenerekherbirinde
onar rat bulunan toplam dört grup oluşturuldu. Kontrol grubuna herhangi bir
işlem uygulanmadı, iskemi grubuna kronik iskemi modeli oluşturuldu, serum
fizyolojik grubuna iskemi oluşturulduktan sonra serum fizyolojik enjeksiyonu
yapıldı, lipoteikoik asit grubuna iskemi oluşturulmasını takiben intramusküler
lipoteikoik asit enjeksiyonu yapıldı. Ratlar laboratuvar ortamında otuz gün
boyunca vital bulgular ve enfeksiyon açısından takip edildikten sonra anestezi
verilmesini takiben ekssanguinasyon yöntemiyle sakrifiye edildi, toraks ön
duvarı açıldı, kalp içerisinden enjektör yardımıyla 5 cc kan alınarak
biyokimyasal analizler yapıldı, toplanan kan örneklerinde proanjiyogenik
faktörlerden vasküler endotel büyüme faktörü ve anjiopoietin-1,
anti-anjiyogenik faktörlerden endostatin ve anjiostatinin serumdaki düzeyleri
ölçüldü. Ratların arka bacaklarından gastroknemius kasları çıkarılarak
histopatolojik analizler yapıldı, alınan kas dokusu örnekleri ışık ve elektron
mikroskobisi ile değerlendirildi. Bulgular: Yapılan değerlendirmeler sonucunda, lipoteikoik asit grubunda diğer
gruplara göre yeni oluşan kapillerite sayısı, anjiopoietin-1 düzeyi, endostatin
ve anjiostatin bakımından anlamlı derecede artış tespit edildi (p<0.05). Sonuç: Bulgularımız, kronik iskemi
modelinde lipoteikoik asitin anjiyogenezisi etkin bir biçimde sağladığını, aynı
zamanda proanjiyogenik ve anti-anjiyogenik sitokinlerin her ikisinde birden
artışa yol açarak anjiyogenezisin kontrollü bir süreçte geliştiğini telkin
etmektedir.
Keywords
References
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- 2. Sunderkotter C, Steinbrink K, Goebeler M, Bhardwaj R, Sorg C. Macrophages and angiogenesis. J Leukoc Biol 1994;55:410-422.
- 3. Alkan ML, Beachey EH. Excretion of lipoteichoic acid by group A streptococci. Influence of penicillin on excretion and loss of ability to adhere to human oral mucosal cells. J Clin Invest 1978;61(3):671–677.
- 4. Ginsburg I . Role of lipoteichoic acid in infection and inflammation. Lancet Infect Dis 2002;2:171–179.
- 5. Morath S, Wendel A, Hartung T. Interscience Conference on Antimicrobial Agents and Chemotherapy. Identification of Lipoteichoic Acid as a Major Cytokine-Stimulatory Principle of Staphylococcus aureus. Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother 1999;29(39):73.
- 6. Opitz B, Schroder NW, Spreitzer I, Michelsen KS, Kirschning CJ, Hallatschek W. Toll-like receptor-2 mediates Treponema glycolipid and lipoteichoic acid-induced NF-kappaB translocation. J Biol Chem 2001;276(25):22041-22047.
- 7. Keller R, Fischer W, Keist R, Bassetti S. Macrophage response to bacteria: induction of marked secretory and cellular activities by lipoteichoic acids. Infect Immun 1992;60:3664-3672.
- 8. Schwandner R, Dziarski R, Wesche H, Rothe M, Kirschning CJ. Peptidoglycan and Lipoteichoic Acid induced Cell Activation Is Mediated by Toll-like Receptor 2. J Biol Chem 1999;274(25):17406-17409.
- 9. Auguet M, Lonchampt MO, Delaflotte S, Goulin-Schulz J, Chabrier PE, Braquet P. Induction of nitric oxide synthase by lipoteichoic acid from Staphylococcus aureus in vascular smooth muscle cells. FEBS Lett 1992;297:183–185.
- 10. Lın CH, Sheu SY, Lee HM, Ho YS, Lee WS, Ko WC, Sheu JR. Involvement of protein kinase C-γ in IL-1β-induced cyclooxygenase-2 expression in human pulmonary epithelial cells. Mol Pharmacol 2000;57:36–43.
- 11. Cooke JP. NO and angiogenesis. Atheroscler Suppl 2003;4(4):53-60.
- 12. Yang Z, von Ballmoos MW, Diehm N, Baumgartner I, Kalka C, Di Santo S. Call for a reference model of chronic hind limb ischemia to investigate therapeutic angiogenesis. Vascul Pharmacol 2009;51(4):268-274.
- 13. Kim MS, Lee CS, Hur J, Cho HJ, Jun SI, Kim TY, Lee SW, Suh JW, Park KW, Lee HY, Kang HJ, Lee DS, Koh GY, Nakagami H, Morishita R, Park YB, Kim HS. Priming with angiopoietin-1 augments the vasculogenic potential of the peripheral blood stem cells mobilized with granulocyte colony-stimulating factor through a novel Tie2/Ets-1 pathway. Circulation 2009;120(22):2240-2250.
- 14. Shyu KG, Manor O, Magner M, Yancopoulos GD, Isner JM. Direct Intramuscular Injection of plasmid DNA encoding angiopoietin-1 but not angiopoietin-2 augments revascularization in the rabbit ischemic hindlimb. Circulation 1998;98: 2081-2087.
- 15. Nykänen AI, Krebs R, Saaristo A, Turunen P, Alitalo K, Ylä-Herttuala S, Koskinen PK, Lemström KB. Angiopoietin-1 protects against the development of cardiac allograft arteriosclerosis. Circulation 2003;107:1308-1314.
- 16. Liekens S, De Clercq E, Neyts J. Angiogenesis: regulators and clinical applications. Biochem Pharmacol 2001;61:253-270.
- 17. Jakeman LB, Winer J, Bennet GL, Altar CA, Ferrera N. Binding sites for vascular endothelial growth factor are localized on endothelial cells in adult rat tissues. J Clin Invest 1992;89:244-253.
- 18. Sato T, El-Assal ON, Ono T, Yamanoi A, Dhar DK, Nagasue N. Sinusoidal endothelial cell proliferation and expression of angiopoietin/Tie family in regenerating rat liver. J Hepatol 2001;34:690-698.
- 19. Merino G, Fujino Y, Hanashiro RK. Lipoteichoic acid as an inducer of acute uveitis in the rat. IOVS 1998;39(7):1251-1256.
Abstract
References
- 1. Sasaki K, Murohara T, Ikeda H, Sugaya T, Shimada T, Shintani S, Imaizumi T. Evidence for the importance of angiotensin II type 1 receptor in ischemia-induced angiogenesis. J Clin Invest 2002;109:603-611.
- 2. Sunderkotter C, Steinbrink K, Goebeler M, Bhardwaj R, Sorg C. Macrophages and angiogenesis. J Leukoc Biol 1994;55:410-422.
- 3. Alkan ML, Beachey EH. Excretion of lipoteichoic acid by group A streptococci. Influence of penicillin on excretion and loss of ability to adhere to human oral mucosal cells. J Clin Invest 1978;61(3):671–677.
- 4. Ginsburg I . Role of lipoteichoic acid in infection and inflammation. Lancet Infect Dis 2002;2:171–179.
- 5. Morath S, Wendel A, Hartung T. Interscience Conference on Antimicrobial Agents and Chemotherapy. Identification of Lipoteichoic Acid as a Major Cytokine-Stimulatory Principle of Staphylococcus aureus. Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother 1999;29(39):73.
- 6. Opitz B, Schroder NW, Spreitzer I, Michelsen KS, Kirschning CJ, Hallatschek W. Toll-like receptor-2 mediates Treponema glycolipid and lipoteichoic acid-induced NF-kappaB translocation. J Biol Chem 2001;276(25):22041-22047.
- 7. Keller R, Fischer W, Keist R, Bassetti S. Macrophage response to bacteria: induction of marked secretory and cellular activities by lipoteichoic acids. Infect Immun 1992;60:3664-3672.
- 8. Schwandner R, Dziarski R, Wesche H, Rothe M, Kirschning CJ. Peptidoglycan and Lipoteichoic Acid induced Cell Activation Is Mediated by Toll-like Receptor 2. J Biol Chem 1999;274(25):17406-17409.
- 9. Auguet M, Lonchampt MO, Delaflotte S, Goulin-Schulz J, Chabrier PE, Braquet P. Induction of nitric oxide synthase by lipoteichoic acid from Staphylococcus aureus in vascular smooth muscle cells. FEBS Lett 1992;297:183–185.
- 10. Lın CH, Sheu SY, Lee HM, Ho YS, Lee WS, Ko WC, Sheu JR. Involvement of protein kinase C-γ in IL-1β-induced cyclooxygenase-2 expression in human pulmonary epithelial cells. Mol Pharmacol 2000;57:36–43.
- 11. Cooke JP. NO and angiogenesis. Atheroscler Suppl 2003;4(4):53-60.
- 12. Yang Z, von Ballmoos MW, Diehm N, Baumgartner I, Kalka C, Di Santo S. Call for a reference model of chronic hind limb ischemia to investigate therapeutic angiogenesis. Vascul Pharmacol 2009;51(4):268-274.
- 13. Kim MS, Lee CS, Hur J, Cho HJ, Jun SI, Kim TY, Lee SW, Suh JW, Park KW, Lee HY, Kang HJ, Lee DS, Koh GY, Nakagami H, Morishita R, Park YB, Kim HS. Priming with angiopoietin-1 augments the vasculogenic potential of the peripheral blood stem cells mobilized with granulocyte colony-stimulating factor through a novel Tie2/Ets-1 pathway. Circulation 2009;120(22):2240-2250.
- 14. Shyu KG, Manor O, Magner M, Yancopoulos GD, Isner JM. Direct Intramuscular Injection of plasmid DNA encoding angiopoietin-1 but not angiopoietin-2 augments revascularization in the rabbit ischemic hindlimb. Circulation 1998;98: 2081-2087.
- 15. Nykänen AI, Krebs R, Saaristo A, Turunen P, Alitalo K, Ylä-Herttuala S, Koskinen PK, Lemström KB. Angiopoietin-1 protects against the development of cardiac allograft arteriosclerosis. Circulation 2003;107:1308-1314.
- 16. Liekens S, De Clercq E, Neyts J. Angiogenesis: regulators and clinical applications. Biochem Pharmacol 2001;61:253-270.
- 17. Jakeman LB, Winer J, Bennet GL, Altar CA, Ferrera N. Binding sites for vascular endothelial growth factor are localized on endothelial cells in adult rat tissues. J Clin Invest 1992;89:244-253.
- 18. Sato T, El-Assal ON, Ono T, Yamanoi A, Dhar DK, Nagasue N. Sinusoidal endothelial cell proliferation and expression of angiopoietin/Tie family in regenerating rat liver. J Hepatol 2001;34:690-698.
- 19. Merino G, Fujino Y, Hanashiro RK. Lipoteichoic acid as an inducer of acute uveitis in the rat. IOVS 1998;39(7):1251-1256.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Articles |
| Authors | |
| Publication Date | December 25, 2018 |
| Submission Date | June 21, 2018 |
| Acceptance Date | August 16, 2018 |
| Published in Issue | Year 2018 Volume: 11 Issue: 3 |
Cite
MEU Journal of Health Sciences Assoc was began to the publishing process in 2008 under the supervision of Assoc. Prof. Gönül Aslan, Editor-in-Chief, and affiliated to Mersin University Institute of Health Sciences. In March 2015, Prof. Dr. Caferi Tayyar Şaşmaz undertook the Editor-in Chief position and since then he has been in charge.
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