Determination of Essential Oil Components of Two Different Varieties Belonging to Subspecies of Mentha longifolia subsp. typhoides

Year 2022, , 131 - 137, 26.12.2022

Osman Gedik , Yusuf Ziya Kocabaş , Orçun Çınar

https://doi.org/10.53518/mjavl.1096208

Abstract

Mentha, which is in the Lamiaceae family, is known to be a plant rich in essential oil and secondary metabolites. In addition, mint, which contains high levels of phenolic and flavonoid compounds, is a very important species in both health and commercial areas. The gradual increase in the world population also increases the demand for both the essential oil and secondary metabolites of mint, which is among the medicinal and aromatic plants. In this study, essential oil components of two different varieties, M. longifolia (L.) Hudson subsp. typhoides (Brıq.) Harley var. typhoides (L.) Hudson and M. longifolia (L.) Hudson subsp. typhoides (Brıq.) Harley var. calliantha (Stapf) Brıq., were determined. The study was carried out in the Medical and Aromatic Plants Laboratory of Kahramanmaras Sütcü Imam University Faculty of Agriculture. Essential oil components were determined in the GC/MS device at the West Mediterranean Agricultural Research Institute. While essential oil was obtained from two different plant parts, the flower and leaf, of the M. longifolia subsp. typhoides var. typhoides samples, the essential oil was obtained by using the above-ground parts of the M. longifolia subsp. typhoides var. calliantha samples. Considering the essential oil components of M. longifolia subsp. typhoides var. typhoides samples; 16 different components were determined in the essential oil obtained from the flower, and 19 different essential oil components were determined in the essential oil obtained from the leaves. The main component in flower essential oil is piperitenoneoxide with 56.87%, followed by cis-piperitoneoxide with 15.89% and eucarvone with 11.30%. Cis-piperitoneoxide with 35.69% and piperitoneoxide with 35.20% are the main components in leaf essential oil. In M. longifolia subsp. typhoides var. calliantha samples, the main essential oil component was pulegone with 47.72%, followed by menthone with 17.19%, trans-dihydrocarvone with 7.89%, eucarvone with 7.55%, and piperitone with 5.17%.

Keywords

Mentha, Essential oil, M. longifolia, Flower, Leaf

Mentha longifolia subsp. typhoides Alt Türüne Ait İki Farklı Varyetenin Uçucu Yağ Bileşenlerinin Belirlenmesi

Abstract

Lamiaceae familyası içerisinde yer alan nanenin (Mentha sp.) uçucu yağ ve sekonder metabolit açısından zengin bir bitki olduğu bilinmektedir. Ayrıca fenolik ve flavonoid bileşikleri yüksek oranlarda bulunduran nane, hem sağlık hem de ticari alanda oldukça önemli bir türdür. Dünya nüfusunun giderek artması tıbbi ve aromatik bitkiler arasında olan nanenin gerek uçucu yağına gerekse sekonder metabolitlerine olan talebi de artırmaktadır. Bu çalışmada M. longifolia (L.) Hudson subsp. typhoides (Brıq.) Harley var. typhoides (L.) Hudson ve M. longifolia (L.) Hudson subsp. typhoides (Brıq.) Harley var. calliantha (Stapf) Brıq. olmak üzere iki farklı varyetenin uçucu yağ bileşenleri belirlenmiştir. Çalışma Kahramanmaraş Sütçü İmam Üniversitesi Ziraat Fakültesine ait olan Tıbbi ve Aromatik Bitkiler Laboratuvarı’nda yürütülmüştür. Uçucu yağ bileşenleri Batı Akdeniz Tarımsal Araştırma Enstitüsünde GC/MS cihazında belirlenmiştir. M. longifolia subsp. typhoides var. typhoides örneğinin çiçek ve yaprak olmak üzere iki farklı bitki kısmından uçucu yağ elde edilirken, M. longifolia subsp. typhoides var. calliantha örneğinin herba kısımları kullanılarak uçucu yağ elde edilmiştir. M. longifolia subsp. typhoides var. typhoides örneğinin uçucu yağ bileşenlerine bakıldığında; çiçekten elde edilen uçucu yağda 16 farklı bileşen, yapraktan elde edilen uçucu yağda ise 19 farklı uçucu yağ bileşeni belirlenmiştir. Çiçek uçucu yağında başlıca bileşen %56.87 ile piperitenoneoxide olup bu bileşeni %15.89 ile cis-piperitoneoxide, %11.30 ile eucarvone takip etmektedir. Yaprak uçucu yağında %35.69 ile cis-piperitoneoxide ve %35.20 ile piperitoneoxide başlıca bileşenlerdir. M. longifolia subsp. typhoides var. calliantha örneğinde başlıca uçucu yağ bileşeni %47.72 ile pulegone olup bu bileşeni %17.19 ile menthone, %7.89 ile trans-dihydrocarvone, %7.55 ile eucarvone, %5.17 ile piperitone’nun takip ettiği belirlenmiştir.

Keywords

Mentha, Uçucu yağ, M. longifolia, çiçek, yaprak, Mentha, Essential oil, M. longifolia, Flower, Leaf

References

• Abdelli M, Moghrani H, Aboun A, Maachi R 2016. Algerian Mentha pulegium L. leaves essential oil: chemical composition, antimicrobial, insecticidal andantioxidant activities. Ind. Crop. Prod. 94: 197-205.
• Aksit H, Demirtas I, Telci I, Tarimcilar G 2013. Chemical diversity in essential oil composition of Mentha longifolia (L.) Hudson subsp. typhoides (Briq.) Harley var. typhoides from Turkey. The Journal of Essential Oil Research. 25(5): 430-437.
• Asgharia B, Zengin G, Bahadoric MB, Abbas-Mohammadid M, Dinparast L 2018. Amylase, glucosidase, tyrosinase, and cholinesterases inhibitory, antioxidant effects, and GC-MS analysis of wild mint (Mentha longifolia var. calliantha) essential oil: A natural remedy. European Journal of Integrative Medicine. 22: 44-49.
• Bahadori MB, Zengin G, Bahadori S, Dinparast L, Movahhedin N2018. Phenolic composition and functional properties of wild mint (Mentha longifolia var. calliantha (Stapf) Briq.). International Journal of Food Properties. 21(1): 198-208.
• Bakht J, Shaheen S, Shafi M2014. Antimicrobial potentials of Mentha longifolia by discdiffusion method. Pak. J. Pharm. Sci. 27(4): 939-945.
• Başer KHC, Kürkçüoglu M, Tarimcilar G, Kaynak G 1999. Essential Oils of Mentha Species from Northern Turkey. Journal of Essential Oil Research. 11(5): 579-588.
• Baydar H 2016. Tıbbi ve Aromatik Bitkileri Bilimi ve Teknolojisi (Genişletilmiş 5. Baskı). Süleyman Demirel Üniversitesi Ziraat Fakültesi, Yayın No: 51, Isparta.
• Berber I, Avsar C, Cine N, Bozkurt N, Elmas E2013. Sinop’ta yetişen bazı bitkilerin metanolik ekstraktlarin inantibakteriyal ve antifungal aktivitelerinin belirlenmesi. Karaelmas Science and Engineering Journal. 3(1): 10-16.
• Biswas N N, Saha S, Ali MK2014. Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. Asian Pacific Journal of Tropical Biomedicine. 4(10): 792-797.
• Davis PH 1982. Flora of Turkey and the East Aegean Islands, Vol. 7, p. 390. University Press, Edinburgh.
• Deka H, Deka S, Baruah CK2015. Plant Growth promoting rhizobacteria for value addition: mechanism of action. In: D. Egamberdieva et al. (eds.). Plant-Growth-Promoting Rhizobacteria (PGPR) and Medicinal Plants, Soil Biology 42. Springer International Publishing, Switzerland 305-321.
• Dualı G 2010. Bazı Türk nane (Mentha l.) uçucu yağlarının biyolojik aktiviteleri. Anadolu Üniversitesi, Yüksek Lisans Tezi, Sağlık Bilimleri Enstitüsü, Farmakognozi Anabilim Dalı, Eskişehir, 45s.
• Dzamic AM, Sokovic MD, Ristic MS, Novakovic M, Grujic-Jovanovic S, Tesevic V, Marin PD 2010. Antifungal and antioxidant activity of Mentha longifolia (L.) Hudson (Lamiaceae) essential oil. Botanica Serbica. 34 (1): 57-61.
• Economou KD, Oreopolou O, Thomopoulos CD 1991. Antioxidant activity of some plant extracts of the family Labiatae. J Am Oil Chem Soc. 68: 109-113.
• Elmastaş M, Dermirtas I, Isildak O, Aboul-Enein HY 2006. Antioxidant activity of Scarvone isolated from spear mint (Mentha spicata L. Fam. Lamiaceae). J Liq Chromatogr Relat Technol. 29:1465-1475.
• Fatiha B, Didier H, Naima G, Khodir M, Martin K, Leocadie K, Caroline S, Mohamed C, Pierre D2015. Phenolic compsoition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds. (Lamiacea). Ind. Crops Prod. 74: 722-730.
• Güllüce M, Şahin F, Sökmen M, Özer H, Daferera D, Sökmen A, Polissiou M, Adiguzel A, Özkan H 2007. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chem. 103:1449–56.
• Harley RM, Atkins S, Budantsev A, Cantino PD, Conn BJ, Grayer R, Harley MM de Kok R, Krestovskaja T, Morales R, Paton AJ, Ryding O, Upson T 2004. Labiatae. In: Kubitzki, K. (ed.), TheFamiliesandGenera of Vascular Plants, vol. 7, pp. 167-275. Springer-Verlag, Berlin.
• Kouhila M, Belghit A, Daguen M, Boutaleb BC 2001. Experimental determination of the sorptionisotherms of mint (Mentha viridis), sage (Salvia officinalis) and verbena (Lippia citriodora). J.FoodEngr. 47:281–287.
• Llorens-Molina JA, Vacas S, Castell V, Verdeguer M 2020. Seasonal variations of essential oils from five accessions of Mentha longifolia (L.) L. with selected chemical profiles. Journal of Essential Oil Research. 32(5): 419-428.
• Mahboubi M, Haghi G 2008. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. J Ethnopharmacol. 119:325–327.
• Okut N, Yağmur M, Selçuk N, Yıldırım B 2017. Chemical composition of essential oil of Mentha longifolia L. subsp. longifolia Growing Wild. Pak. J. Bot. 49(2): 525-529.
• Özgüven M, Kırıcı S 1999. Farklı ekolojilerde nane (Mentha) türlerinin verim ile uçucu yağ oran ve bileşenlerinin araştırılması. Tr. J. of Agriculture and Forestry. 23: 465-472.
• Saleem M, Alam A, Sultana S 2000. Attenuation of benzoylperoxide-mediated cut aneousoxidative stress and hyperproliferative response by the prophylactic treatment of mice with spearmint (Mentha spicata). FoodChemToxicol. 38:939–948.
• Salihila J, Nuro A, Dervishi A, Peçi D, Shëngjergji D 2018. Chemical composition of Mentha longifolia essential oil from Albania populations. International Journal of Engineering and Applied Sciences. 5(4):63-66.
• Sönmez E, Köse YB 2008. Morpho-anatomical investigations on Ajugapostii Briq and Ajugarelicta PH Davis. Biological Diversity and Conservation. 10(1): 39-49.
• Uysal Bayar F, Çınar O 2020. Yield and quality parameters of some cultivated Origanum spp. species. Derim. 37(1): 10-17.
• Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I 2006. Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry. 67: 1249-1255.