Detection of some antioxidant enzyme activities in apricot fruit grown in Van region from Turkey

Year 2020, Volume: 8 Issue: 1, 17 - 21, 30.06.2020

Mustafa Bilici

Abstract

In this study, it was aimed to determine antioxidant enzyme activity in apricot fruit. In the studies carried out, apricot has been reported to have protective effects such as antioxidant, anti-aging, anticancer and antivirus.It also protects brain cells. Apricot kernel also contains some substances with antioxidant properties. In other studies carried out, it has been also reported to be beneficial to heart health. The antioxidant property of this fruit is quite high. Its structure contains many vitamins, minerals and enzymes. For this purpose, it is aimed to determine the activity of superoxide dismutase (SOD) which is one of the enzyme activities thought to be present in apricot fruit. Furthermore, oxidative stress indicator and the level of malondialdehyde (MDA), which is the end product of lipid peroxidation, were determined. In this study, antioxidant enzyme activity and lipid peroxidation were determined by spectrophotometric method. Then, the results obtained were analyzed by using multidimensional statistical methods and the results were discussed in a multidimensional manner.

Keywords

Apricot, Fruit, Antioxidant Enzyme, Oxidative Stress, MDA, SOD

References

• [1] T.C Orman ve Su İşleri Bakanlığ Orman Genel Müdürlüğü Yayınları Maviyemiş Likapa Eylem Planı 2015-2019.
• [2] A.R,Galambos, The effects of Blueberry anthocyanıdıns on antoxidant enzyme activity in human HEPG2 cells.A Thesis submitted of the Graduate Faculty of The University fo Georgia.2012.
• [3] T.Uruk,S.Kahraman,”Yaban Mersini (Vaccinim myrtillus) Antioksidan aktivitesinin araştırılması.”Iğdır.Üniversitesi Fen Bilimleri Enst.Der.71(4):77-83.2017.
• [4] Mot CA, Dumitrescu SR, Sarbu C,.“Rapid and effective evaluation of the antioxidant capacity of propolis extracts using DPPH bleaching kinetic profiles, FT-IR and UV-VIS spectroscopic data”. Journal of Food Composite and Analysis, 24, 516-522.2011.
• [5] A, Güvenç.,H, Gül., E, “Uzun Yaban mersini posasının antioksidan kapasitesi ve trans-resveratrol derişimi üzerine ses ötesi dalgaların etkisinin incelenmesi.” BAP Proje, 2011.
• [6] S.Skrovankova, P.Sumezynki, J.Mlcek, T.Jurikova and J.Sochor.”Bioactive Compounds and Antioxidant Activity in Different Types of Berries” Int.J.Mol.Sci.16, 24673-24706.2015.
• [7] F.Işık, Ü.Urgancı, F.Turan.” Yaban Mersini İlaveli Muffin Keklerin Bazı Kimyasal, Fiziksel ve Duyusal Özellikleri” Akademik Gıda 15 (2) 130-138, 2017.
• [8] İ.Tosun, S. Yüksel,” Üzümsü meyvelerin antioksidan kapasitesi”. Gıda 28(3): 305-311. 2003.
• [9] İ.Yılmaz, “Antioksidan içeren bazı gıdalar ve oksidatif stress”. İnönü Üniversitesi Tıp Fakültesi Dergisi 17(2): 143-153.2010.
• [10] E.Derviş,” Oral antioksidanlar. Dermatoz “.2(1): 263-267.2011.
• [11] A.Mavi,” İnsan eritrosit ve lökositlerinden süperoksit dismutaz enzimininsaflaştırılması ve bazı ilaçların enzim üzerine etkilerinin incelenmesi”. Doktora Tezi,Atatürk Üniversitesi Fen Bilimleri Enstitüsü, s.52-53.2005.
• [12] E.R., Stadtman, “ Importance of individuality in oxidative stress and aging”. FreeRadical Biology and Medicine, 33, 597-604.2002.
• [13] E.Çaylak, “ Hayvan ve bitkilerde oksidatif stres ile antioksidanlar. Biyokimya” AD, Çankırı Karatekin Üniversitesi 18200 Tıp Araştırmaları Dergisi: 9: (1) : 73-83.2011.
• [14] C.Rice-Evans., R., Re., N. Pellegrini., A.Proteggente.,A. Pannala., M.Yang. “ Antioxidant activity applying an improved ABTS radical cation decolorization assay”, Free Radic. Biol. Med., 26, 1231-1237.1999.
• [15] J.Bergmeyer, and M.Grabl,.,Methods of Enzymatic Analysis (Third Edition), Germany. pp.190-302, 1983. [16] H. Aebi., Catalase in vitro. Enzymol;105:121-6. 1984.
• [17] İ.Akkuş., Serbest Radikaller ve Fizyopatolojik Etkileri, Mimoza Yayınları,Konya, 1995, syf 68.
• [18] A.Becker, Controlling als Praxis: Eine structurationsTheoretische Perspektive auf Controlling. In E. Scherm & G. Pietsch (Hrsg.), Controlling: Theorien und Konzeptionen pp. 753-777.2004.
• [19] JMC. Gutteridge.,“Lipid peroxidation and antioxidants as biomarkers of tissue damage”. Clin. Chem., 41: 1819-28.1995.
• [20] E. Güler.,H. Dedeakayoğulları., A.Kılınç., A.S. Yalçın., ,” Oksidatif stresin belirlenmesinde yeni bir yaklaşım” 23.ulusal biyokimya kongresi. 2011.
• [21] Halliwell.,J. M. C. Gutteridge.,” Iron and oxygen: A biologically damaging mixture”. Acta Paediatrica Scandinavica Suppl, 361, 78-85.1989.
• [22] JM.Gutteridge.,“Lipid peroxidation and antioxidants as biomarkers of tissue damage.” Clin Chem. Dec;41(12 Pt 2):1819-28. Review. 1995.
• [23] Y. Sun., LW. Oberley.,Y. Li., “A simple method for clinical assay of superoxide dismutase.”Clin Chem. Mar;34(3):497-500. 1988.
• [24] H. Demir., A. Güler., İ. Yörük., C. Demir., “ Ceviz Meyvesinde Bazı Antioksidan Enzim Aktivitelerinin Belirlenmesi” ISMSIT 2018 Oct. 19-21.2018 Turkiye.
• [25] Haznedaroğlu, M.Z., Öztürk, T. ve Konyalıoğlu, S. 2002. Salvia smyrnaea boiss. uçucu yağının antioksidan ve antimikrobiyal aktivitesi. Bitkisel İlaç Hammaddeleri Toplantısı, Bildiriler, Eskişehir.
• [26] Tosun, İ. and Üstün, Ş. 2003. An investigation about antioxidant capacity of fruit nectars. Pakistan Journal of Nutrition, 2(3): 167-169.