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Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues

Year 2015, Volume: 2 Issue: 4, 244 - 53, 07.04.2015

Mustafa Atac , Saban Sezen Mustafa Bilge Berrin Isik Mustafa Arslan , Faruk Comu , Mustafa Kavutcu , Dervis Yilmaz

Abstract

Background and aim: The aim of study was to assess the oxidative status in rat hepatic and renal tissues after intraperitoneal administration of acetaminophen (AP) versus lornoxicam (L).

Materials and Methods: For this purpose 18 Wistar Albino rats were randomly divided into 3 groups; each group consists of 6 rats. Group Control (Group C) remain untreated, comprises healthy rats. Group AP received AP (100 mg kg-1) and Group L received L (1.3 mg kg-1) intraperitoneally. Oxidative status was evaluated by MDA, SOD, GST and CAT in hepatic and renal tissues. Furthermore histopathalogical evaluation was performed in both tissues.

Results: The lornoxicam received rats (Group L) showed significantly increased level of MDA and GST [(p=0.015), (p=0.048) respectively],decreased level of SOD (p=0.02)in liver tissue. Renal tissue MDA, SOD and GST activity and CAT levels were similar in groups [(p=0.168), (p=0.270), (p=0.686) respectively]. Histopathalogically Group AP and Group L more damaged than in Group C. Hepatic injury was moderate level in two groups. Minimal injury was observed in group AP. Renal injury in group L more than the Group AP.

Conclusion: The results suggest that hepatotoxic effects of lornoxicam more than the AP while no remarkable difference nephrotoxicity.

Keywords

Oxidative stress; Acetaminophen; Lornoxicam; Rat; Hepatic Renal

References

  • Litovitz TL, Klein-Schwartz W, Rodgers GC Jr, et al. 2001 annual report of the American Association of Poison Control Centers Toxic Exposure Surveillance System. Am J Emerg Med 2002; 20: 391-452.
  • Waters E, Wang JH, Redmond HP, et al. Role of taurine in preventing acetaminophen-induced hepatic injury in the rat. Am J Physiol Gastrointest Liver Physiol 2001; 280: 1274-1279.
  • Noriega GO, Ossola JO, Tomaro ML, Batlle AM. Effect of acetaminophen on heme metabolism in rat liver. Int J Biochem Cell Biol 2000; 32: 983-991.
  • Gambaro G, Perazella MA. Adverse renal effects of antiinflammatory agents: evaluation of selective and nonselective cyclooxygenase inhibitors. J Int Med 2003; 253: 643-52.
  • Arslan M, Comu FM, Isik B, Atac MS, Yilmaz D. The evaluation of effects of lornoxicam on blood flow and erythrocythe deformability in comparison with i.v. paracetamol in rats. Bratisl Med J 2012; 113: 211-213.
  • Andrzej P, Monika W Agniescka S Wejeiech G, Patrycja LK, Jerzy M. NSAID acute liver failure-A case Report. J. Chromatogr. B. Analyte. technol. Biomed. cif. sci, 2011; 879:573-578.
  • Turner P, Johnston A. Clinical pharmacokinetic studies withlornoxicam. Postgrad. Med. J. 1990; 66: 28–9.
  • Bianchi M, Panerai AE. Effects of lornoxicam, piroxicam, andmeloxicam in a model of thermal hind paw hyperalgesia induced by formalininjection in rat tail. Pharmacol Res 2002; 45: 101–105.
  • Pohlmeyer-Esch G, Mehdi N, Clarke D, Radhofer-Welte S.Evaluation of chronic oral toxicity and carcinogenic potential of lornoxicamin rats. Food Chem. Toxicol. 1997;35: 909–922.
  • Sen S, Doger FK, Sen S, Aydin ON, Karul A, Dost T. The protective effect of nitroglycerin on gastrointestinal and renal side effects of lornoxicam in rats. Eur J Pharmacol 2006; 541: 191-7.
  • Abdel-Wahhab MA, Nada SA, Arbid MS. Ochratoxicosis: Prevention of developmental toxicity by L-methionine in rats m. Journal of applied toxicology. J Appl Toxicol 1999; 19: 7-12.
  • Van Ye TM, Roza AM, Pieper GM, Henderson J Jr, Johnson JP, Adams MB. Inhibition of intestinal lipid peroxidation does not minimize morphological damage. J Surg Res 1993; 55:553-558.
  • Durak I, Canbolat O, Kavutcu M, Öztürk HS, Yurtarslanı Z. Activities of total, cytoplasmic and mihochondrial superoxide dismutase enzymes in sera and pleural fluids from patient with lung cancer. J Clin Lab Anal 1996; 10: 17-20.
  • Aebi H. Catalase. In: H.U.Bergmeyer (Ed): Methods of Enzymatic Analysis, Academic Press , New York and London, 1974; pp.673-677.
  • Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130– 9.
  • John CM, Shukla R, Jones CA. Using NSAID in volume depletedchildren can precipitate acute renal failure. Arch Dis Child 2007;92: 524–526
  • Onay OS, Erçoban HS, Bayrakçı US, Melek E, Cengiz N, Baskin E.Acute, reversible nonoliguric renal failure in two children associatedwith analgesic –antipyretic drugs. Pediatric Emergency Care 2009;25 (4): 263-266
  • Banal F, Lecoules S, Carmoi T, Thomas L, Bonnefoy S, Bechade D,Desrame J, Algayres JP. Acute renal insufficiency after flurbiprofentreatment in a patient treated with angiotensin converting enzymeinhibitor. Rev Med Interne 2008; 29: 593-595
  • Gökceoglu Uslu A, Doğan ÇS, Çomak E, Akkaya B, Koyun M, Akman S. Acute tubulointerstitial nephritis: presentation of four cases. Turk Neph Dial Transpl 2013; 22: 106-110.
  • Schwartz GJ, Muñoz A, Schneider MF, Mak RH, Kaskel F, WaradyBA, Furth SL. New equations to estimate GFR in children withCKD. J Am Soc Nephrol 2009;20:629-637
  • Krause I, Cleper R, Einstein B, Davidovitis M. Acute renal failure,associated with non-steroidal anti-inflamatory drugs in healthychildren. Pediatr Neprol 2005;20:1295-1298
  • Duh PD. Antioxidant activity of Burdock, its scavenging effecton free radical and active oxygen. J Am Oil Chem Soc 1998; 75:455‐458.
  • Basma K. Ramadanand Mona F. Schaalan. The renoprotective effect of clover flowers honey on paracetamol-induced nephrotoxicity in adult male albino rats. Life Science Journal 2011; 8:589-596.
  • Koçak A, Gökçimen A, Yılmaz N, Vural H. Evaluation of the effect of acetaminophen at different doses on hepatic nitric oxide synthase enzyme by using immunohistochemical and biochemical methods. Nobel Med 2011; 7: 88-95.
  • Lores Arnaiz S, Llesuy S, Cutrín JC, Boveris A. Oxidative stress by acute acetaminophen administration in mouse liver. Free Radic Biol Med 1995; 19: 303-310.
  • Sheeba Rani M, Emmanuel S, Sreekanth MR, Ignacimuthu S. Evaluation of in vivo antioxidant and hepatoprotective activity of cassia occidentalis linn, against paracetamol-induced liver toxicity in rats. Int J Pharmacy Pharm Sci 2010;2:67-70.
  • Wendel A, Feuerstein S, Konz KH. Acute paracetamol intoxication of starved mice leads to lipid peroxidation in vivo. Biochem Pharmacol 1979; 28: 2051-2055.
  • Knight TR, Fariss MW, Farhood A, Jaeschke H. Role of lipid peroxidation as a mechanism of liver injury after acetaminophen overdose in mice. Toxicol Sci 2003; 76: 229–236.
  • Linares MV, Belles M, Albina ML, Sirvent JJ, Sanchez DJ. Assessment of the pro-oxidant activity of uranium in kidney and testis of rats. Toxicol Lett 2006; 167:152-61.
  • Shayiq RM, Roberts DW, Rothstein K, Snawder JE,Benson W, Ma X et al. Repeat exposure to incrementaldoses of acetaminophen provides protection againstacetaminophen-induced lethality in mice: anexplanation for high acetaminophen dosage in humanswithout hepatic injury. Hepatology 1999; 29: 451–463.
  • Stern S, Bruno M, Hennig G, Horton R, Roberts J, Cohen S. Contribution of acetaminophen-cysteine to acetaminophen nephrotoxicity in CD-1 mice. 1. Enhancement of acetaminophen nephrotoxicity by acetaminophen–cysteine. Toxicl Appli Pharmacol 2005; 202: 151-9.
  • Kathleen AS, Michael SL. Effects of deracoxib or buffered aspirin on thegastric mucosa of healthy dogs. J Vet Intern Med 2006;20:1291-1296.
  • Chung H, Kim HJ, Jang KS, Kim M, Yang J, et al. comprehensive analysisof differential gene expression profiles on diclofenac-induced acute mouse liverinjury and recovery. Toxicology Letters 2006;166: 77-87.
  • Hummdi LA, Habashi SA. Histological and ultrastructure studies on the liver changes induced by xefo drug (lornoxicam) of adult female mice. Foreign Agricultural Relations (FAR), Egypt, 29th Nov – 1st Dec 2010: 551– 575
  • LeBail B, Bioulac-sage P, Senuila R, et al. Fine structure of hepatic sinusoidssinusoids cells in disease. J Electron Microsc Tech 1990;14:257-282.
  • Rabab R El-zoghby, Mona F. Biochemical and histopathological changes of lornoxicam in albino rats. Proc. of the 5th Animal Wealth Research Conf. in the Middle East & North Africa. 2012: 254- 271.
  • Radhofer–Welte S. Rabasseda X. Loronoxicam, a new potent NSAID with an improvedtolerability profile. Drugs Today 2000; 36:55-76.
  • Aydin G, Gokcimen A, Oncu M, Cicek E, Karahan N, Gokalp O. Histopathological changes in liver and renal tissues induced by different doses of diclofenicsodium in rats. Turk J Vet Anim Science 2003;(27) :1131-1142.

Effect of acetaminofen versus lornoxicam administration on oxidative stress in rat hepatic and renal tissues

Year 2015, Volume: 2 Issue: 4, 244 - 53, 07.04.2015

Mustafa Atac , Saban Sezen Mustafa Bilge Berrin Isik Mustafa Arslan , Faruk Comu , Mustafa Kavutcu , Dervis Yilmaz

Abstract

References

  • Litovitz TL, Klein-Schwartz W, Rodgers GC Jr, et al. 2001 annual report of the American Association of Poison Control Centers Toxic Exposure Surveillance System. Am J Emerg Med 2002; 20: 391-452.
  • Waters E, Wang JH, Redmond HP, et al. Role of taurine in preventing acetaminophen-induced hepatic injury in the rat. Am J Physiol Gastrointest Liver Physiol 2001; 280: 1274-1279.
  • Noriega GO, Ossola JO, Tomaro ML, Batlle AM. Effect of acetaminophen on heme metabolism in rat liver. Int J Biochem Cell Biol 2000; 32: 983-991.
  • Gambaro G, Perazella MA. Adverse renal effects of antiinflammatory agents: evaluation of selective and nonselective cyclooxygenase inhibitors. J Int Med 2003; 253: 643-52.
  • Arslan M, Comu FM, Isik B, Atac MS, Yilmaz D. The evaluation of effects of lornoxicam on blood flow and erythrocythe deformability in comparison with i.v. paracetamol in rats. Bratisl Med J 2012; 113: 211-213.
  • Andrzej P, Monika W Agniescka S Wejeiech G, Patrycja LK, Jerzy M. NSAID acute liver failure-A case Report. J. Chromatogr. B. Analyte. technol. Biomed. cif. sci, 2011; 879:573-578.
  • Turner P, Johnston A. Clinical pharmacokinetic studies withlornoxicam. Postgrad. Med. J. 1990; 66: 28–9.
  • Bianchi M, Panerai AE. Effects of lornoxicam, piroxicam, andmeloxicam in a model of thermal hind paw hyperalgesia induced by formalininjection in rat tail. Pharmacol Res 2002; 45: 101–105.
  • Pohlmeyer-Esch G, Mehdi N, Clarke D, Radhofer-Welte S.Evaluation of chronic oral toxicity and carcinogenic potential of lornoxicamin rats. Food Chem. Toxicol. 1997;35: 909–922.
  • Sen S, Doger FK, Sen S, Aydin ON, Karul A, Dost T. The protective effect of nitroglycerin on gastrointestinal and renal side effects of lornoxicam in rats. Eur J Pharmacol 2006; 541: 191-7.
  • Abdel-Wahhab MA, Nada SA, Arbid MS. Ochratoxicosis: Prevention of developmental toxicity by L-methionine in rats m. Journal of applied toxicology. J Appl Toxicol 1999; 19: 7-12.
  • Van Ye TM, Roza AM, Pieper GM, Henderson J Jr, Johnson JP, Adams MB. Inhibition of intestinal lipid peroxidation does not minimize morphological damage. J Surg Res 1993; 55:553-558.
  • Durak I, Canbolat O, Kavutcu M, Öztürk HS, Yurtarslanı Z. Activities of total, cytoplasmic and mihochondrial superoxide dismutase enzymes in sera and pleural fluids from patient with lung cancer. J Clin Lab Anal 1996; 10: 17-20.
  • Aebi H. Catalase. In: H.U.Bergmeyer (Ed): Methods of Enzymatic Analysis, Academic Press , New York and London, 1974; pp.673-677.
  • Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130– 9.
  • John CM, Shukla R, Jones CA. Using NSAID in volume depletedchildren can precipitate acute renal failure. Arch Dis Child 2007;92: 524–526
  • Onay OS, Erçoban HS, Bayrakçı US, Melek E, Cengiz N, Baskin E.Acute, reversible nonoliguric renal failure in two children associatedwith analgesic –antipyretic drugs. Pediatric Emergency Care 2009;25 (4): 263-266
  • Banal F, Lecoules S, Carmoi T, Thomas L, Bonnefoy S, Bechade D,Desrame J, Algayres JP. Acute renal insufficiency after flurbiprofentreatment in a patient treated with angiotensin converting enzymeinhibitor. Rev Med Interne 2008; 29: 593-595
  • Gökceoglu Uslu A, Doğan ÇS, Çomak E, Akkaya B, Koyun M, Akman S. Acute tubulointerstitial nephritis: presentation of four cases. Turk Neph Dial Transpl 2013; 22: 106-110.
  • Schwartz GJ, Muñoz A, Schneider MF, Mak RH, Kaskel F, WaradyBA, Furth SL. New equations to estimate GFR in children withCKD. J Am Soc Nephrol 2009;20:629-637
  • Krause I, Cleper R, Einstein B, Davidovitis M. Acute renal failure,associated with non-steroidal anti-inflamatory drugs in healthychildren. Pediatr Neprol 2005;20:1295-1298
  • Duh PD. Antioxidant activity of Burdock, its scavenging effecton free radical and active oxygen. J Am Oil Chem Soc 1998; 75:455‐458.
  • Basma K. Ramadanand Mona F. Schaalan. The renoprotective effect of clover flowers honey on paracetamol-induced nephrotoxicity in adult male albino rats. Life Science Journal 2011; 8:589-596.
  • Koçak A, Gökçimen A, Yılmaz N, Vural H. Evaluation of the effect of acetaminophen at different doses on hepatic nitric oxide synthase enzyme by using immunohistochemical and biochemical methods. Nobel Med 2011; 7: 88-95.
  • Lores Arnaiz S, Llesuy S, Cutrín JC, Boveris A. Oxidative stress by acute acetaminophen administration in mouse liver. Free Radic Biol Med 1995; 19: 303-310.
  • Sheeba Rani M, Emmanuel S, Sreekanth MR, Ignacimuthu S. Evaluation of in vivo antioxidant and hepatoprotective activity of cassia occidentalis linn, against paracetamol-induced liver toxicity in rats. Int J Pharmacy Pharm Sci 2010;2:67-70.
  • Wendel A, Feuerstein S, Konz KH. Acute paracetamol intoxication of starved mice leads to lipid peroxidation in vivo. Biochem Pharmacol 1979; 28: 2051-2055.
  • Knight TR, Fariss MW, Farhood A, Jaeschke H. Role of lipid peroxidation as a mechanism of liver injury after acetaminophen overdose in mice. Toxicol Sci 2003; 76: 229–236.
  • Linares MV, Belles M, Albina ML, Sirvent JJ, Sanchez DJ. Assessment of the pro-oxidant activity of uranium in kidney and testis of rats. Toxicol Lett 2006; 167:152-61.
  • Shayiq RM, Roberts DW, Rothstein K, Snawder JE,Benson W, Ma X et al. Repeat exposure to incrementaldoses of acetaminophen provides protection againstacetaminophen-induced lethality in mice: anexplanation for high acetaminophen dosage in humanswithout hepatic injury. Hepatology 1999; 29: 451–463.
  • Stern S, Bruno M, Hennig G, Horton R, Roberts J, Cohen S. Contribution of acetaminophen-cysteine to acetaminophen nephrotoxicity in CD-1 mice. 1. Enhancement of acetaminophen nephrotoxicity by acetaminophen–cysteine. Toxicl Appli Pharmacol 2005; 202: 151-9.
  • Kathleen AS, Michael SL. Effects of deracoxib or buffered aspirin on thegastric mucosa of healthy dogs. J Vet Intern Med 2006;20:1291-1296.
  • Chung H, Kim HJ, Jang KS, Kim M, Yang J, et al. comprehensive analysisof differential gene expression profiles on diclofenac-induced acute mouse liverinjury and recovery. Toxicology Letters 2006;166: 77-87.
  • Hummdi LA, Habashi SA. Histological and ultrastructure studies on the liver changes induced by xefo drug (lornoxicam) of adult female mice. Foreign Agricultural Relations (FAR), Egypt, 29th Nov – 1st Dec 2010: 551– 575
  • LeBail B, Bioulac-sage P, Senuila R, et al. Fine structure of hepatic sinusoidssinusoids cells in disease. J Electron Microsc Tech 1990;14:257-282.
  • Rabab R El-zoghby, Mona F. Biochemical and histopathological changes of lornoxicam in albino rats. Proc. of the 5th Animal Wealth Research Conf. in the Middle East & North Africa. 2012: 254- 271.
  • Radhofer–Welte S. Rabasseda X. Loronoxicam, a new potent NSAID with an improvedtolerability profile. Drugs Today 2000; 36:55-76.
  • Aydin G, Gokcimen A, Oncu M, Cicek E, Karahan N, Gokalp O. Histopathological changes in liver and renal tissues induced by different doses of diclofenicsodium in rats. Turk J Vet Anim Science 2003;(27) :1131-1142.
There are 38 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Mustafa Atac

Saban Sezen This is me

Mustafa Bilge This is me

Berrin Isik This is me

Mustafa Arslan

Faruk Comu

Mustafa Kavutcu

Dervis Yilmaz This is me

Publication Date April 7, 2015
Published in Issue Year 2015 Volume: 2 Issue: 4

Cite

APA Atac, M., Sezen, S., Bilge, M., Isik, B., et al. (2015). Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues. Medical Science and Discovery, 2(4), 244-53. https://doi.org/10.17546/msd.71259
AMA Atac M, Sezen S, Bilge M, Isik B, Arslan M, Comu F, Kavutcu M, Yilmaz D. Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues. Med Sci Discov. June 2015;2(4):244-53. doi:10.17546/msd.71259
Chicago Atac, Mustafa, Saban Sezen, Mustafa Bilge, Berrin Isik, Mustafa Arslan, Faruk Comu, Mustafa Kavutcu, and Dervis Yilmaz. “Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues”. Medical Science and Discovery 2, no. 4 (June 2015): 244-53. https://doi.org/10.17546/msd.71259.
EndNote Atac M, Sezen S, Bilge M, Isik B, Arslan M, Comu F, Kavutcu M, Yilmaz D (June 1, 2015) Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues. Medical Science and Discovery 2 4 244–53.
IEEE M. Atac, S. Sezen, M. Bilge, B. Isik, M. Arslan, F. Comu, M. Kavutcu, and D. Yilmaz, “Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues”, Med Sci Discov, vol. 2, no. 4, pp. 244–53, 2015, doi: 10.17546/msd.71259.
ISNAD Atac, Mustafa et al. “Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues”. Medical Science and Discovery 2/4 (June 2015), 244-53. https://doi.org/10.17546/msd.71259.
JAMA Atac M, Sezen S, Bilge M, Isik B, Arslan M, Comu F, Kavutcu M, Yilmaz D. Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues. Med Sci Discov. 2015;2:244–53.
MLA Atac, Mustafa et al. “Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues”. Medical Science and Discovery, vol. 2, no. 4, 2015, pp. 244-53, doi:10.17546/msd.71259.
Vancouver Atac M, Sezen S, Bilge M, Isik B, Arslan M, Comu F, Kavutcu M, Yilmaz D. Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues. Med Sci Discov. 2015;2(4):244-53.
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