A RATIONAL SYNTHESIS OF MAGNETIC NANOPARTICLES INCORPORATED HORSERADISH PEROXIDASE NANOFLOWER AND ITS USE FOR THE REMOVAL OF PHENOL THROUGH OXIDATIVE COUPLING REACTION WITH GREAT REUSABILITY

Year 2021, Volume: 7 Issue: 2, 59 - 66, 31.12.2021

Cevahir Altınkaynak , Nalan Özdemir , İsmail Öçsoy

https://doi.org/10.22531/muglajsci.982993

Cited By: 4

Abstract

Herein, we report preparation of magnetic hybrid nanoflower (MhNF) formed of horseradish peroxidase (HRP), copper ions (Cu²⁺) and iron oxide nanoparticles (Fe₃O₄ NPs) with greatly enhanced activity, stability, reusability and applicability for use in removal of phenol from aqueous solution. In this study, pre-synthesized small sized Fe₃O₄ NPs were immobilized to synthesized HRP-Cu²⁺ hybrid nanoflower (hNFs). The synthesized MhNFs were characterized using SEM, EDX and XRD analysis. We also characterized the chemical structures of free HRP and MhNF using FTIR. The protein encapsulation yields of synthesized MhNFs were determined spectrophotometrically and calculated as ~98% for all synthesis conditions. The activities of free HRP, HRP-based non-magnetic hybrid nanoflower (hNF) and MhNF were calculated as 5847.6, 16738.2, and 18830.5 EU/mg, respectively. The optimum pH of the synthesized MhNFs for phenol removal was determined as pH 7. MhNF was easily and rapidly separated from reaction medium by an external magnetic field to be re-used. Even after seven cycles, MhNFs maintained more than 75% of their initial activity. We believe that the repeated use can reduce the capital and operation costs. The results are very promising in terms of increasing efficiency and applicability of HRP in industrial application especially for wastewater treatment.

Keywords

Enzyme-inorganic hybrid nanoflower, enzyme activity, magnetic hybrid nanoflower, peroxidase, phenol removal

Supporting Institution

TÜBİTAK

Project Number

115Z092

Thanks

This work was supported by TÜBİTAK (The Scientific and Technical Research Council of Turkey) project number 115Z092. We thank the technology research and application center of Erciyes University for SEM, EDX, XRD and FTIR analysis. There are no conflicts to declare.

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