Primary and Collagen Supported Scaffold Healing in Rabbit Horizontal Meniscus Ripped Models

Year 2019, Volume: 6 Issue: 1, 21 - 25, 20.03.2019

Cem Yalın Kılınç , Uğur Tiftikçi , Sancar Serbest , Mustafa Türk , Siyami Karahan

Abstract

The aim of this study is to investigate the
potential for improvement of the collagen scaffold in the repair of horizontal
meniscus tears in the rabbit model. Mature 21 New Zealand white male rabbits
weighing 2.5-3 kg were divided into 3 equal groups, with 7 animals in each
group. The medial meniscus was anteriorly horizontal torn from the horn to the
middle. In accordance with the study protocol, Group 1 (n=7); only the
horizontal meniscus was tilted in the control group. Group 2 (n=7); horizontal
meniscus rupture primer repair. Group 3; horizontal meniscus were repaired with
a colloidal scaffold. All experimental animals were sacrificed at the end of
the 12th week. 1/3 anterior area of medial meniscus was measured. Histological
analysis of the tissues was performed with Ishida score consisting of three
components. Surface area of meniscus in group I was significantly lower than
group II (p<0.008) and group III (p<0.002). The quality of the group III
was significantly higher than the other groups (p<0.001). In the tears of
the avascular region, such as the horizontal meniscus tears, scaffolds and
similar materials can provide better healing and meniscal function and function
close to normal anatomic tissue.

Keywords

Collagen Scaffold, Horizontal Rupture, Menuscus Repair, Primary Repair, Rabbit

Tavşanlarda Horizontal Menisküs Yırtık Modellerinde Primer ve Kollajen Skafold ile İyileşme

Abstract

Bu çalışmanın amacı, tavşan modelinde horizontal
menisküs yırtıklarının onarımında kollajen skafold kullanımı sonrası menisküsün
iyileşme potansiyelini araştırmak ve skafold ile tamirin primer tamirden daha
üstün olup olmadığını göstermektir. Ağırlıkları 2.5-3 kg arasında değişen olgun
21 adet New Zealand White cinsi erkek tavşanlar her grupta 7 hayvan olacak
şekilde 3 eşit gruba ayrıldı. Medial menisküs anterior boynuzdan orta kısmına
kadar horizontal yırtık modeli yapıldı. Çalışma protokolüne uygun olarak, Grup
1 (n=7); kontrol grubu sadece horizontal menisküs yırtığı olan tavşanlardan
oluşturuldu. Grup 2 (n=7); horizontal menisküs yırtığa primer tamir yapılırken,
Grup 3; horizontal menisküs yırtığa kollajen skafold ile tamir yapılanlar idi.
Tüm deney hayvanları 12. haftanın sonunda sakrifiye edildi. Medial menisküsün
1/3 anterior alanı ölçüldü. Dokuların histolojik analizi, üç bileşenden oluşan
Ishida skoru ile gerçekleştirildi. Grup I'deki menisküsün yüzey alanı grup II’den
(p<0.008) ve grup III'ten (p<0.002) anlamlı olarak daha düşüktü. Grup
III’deki dokunun kalitesi İshida puanına göre değerlendirildiğinde diğer
gruplardan anlamlı olarak daha yüksekti (p<0.001). Horizontal menisküs
yırtıkları gibi avasküler bölgelerindeki yırtıklarda skafold ve benzeri
materyaller ile daha iyi iyileşme ve normal anatomik dokuya yakın işlev ve
fonksiyonda menisküs elde edilebilir.

Keywords

Horizontal Yırtık, Kollajen Skafold, Menisküs Tamiri, Primer Tamir, Tavşan

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