Poly(N-Methylpyrrole)-Chitosan layers for Glucose Oxidase Immobilization for Amperometric Glucose Biosensor Design

Year 2017, , 123 - 134, 11.11.2017

Gul Ozyilmaz , Ali Tuncay Özyılmaz Rağibe Hülya Akyürekoğlu

https://doi.org/10.28978/nesciences.354825

Cited By: 4

Abstract

In this study, Pt electrode was coated by poly(N-methypyrrol) (PNMP) film, then Glucose Oxidase (GOD) was immobilized onto PNMP layer with thin chitosan (Chi) gel, and finally, the electrode was reacted with glutaraldehyde (GAL) to form crosslinking between –NH2 groups of Chi and GOD to prevent enzyme leakage from Chi. GOD-based electrode was used to measure current response depending on glucose concentration by chronoamperometric method. The preparation of electrode conditions have significant effect on current values which were measured and optimized in presence of glucose, polymer synthesis and GOD immobilization conditions. Therefore, the effect of N-methylpyrrole monomer concentration, scan rate, Chi concentration, GOD concentration and GAL concentration on biosensor response were investigated by classical method. In sight of obtained data, optimal monomer concentration and scan rates for PNMP synthesis were determined as 50 mM and 20 mV/s, respectively. Optimal Chi, GOD and GAL concentrations were found as 1,00%, 4 mg/mL and 0.025 %, respectively. SEM images of Pt, PNMP coated Pt and GOD immobilized Pt electrodes were obtained. After 20 successive uses of same enzyme electrode in 5 mM glucose solution, it was still its 91.3 % of initial activity.

Keywords

Glucose, poly(N-methylpyrrole), amperometric biosensor, glucose oxidase, Chitosan

References

• Abasiyanik, M. F & Senel, M. (2010). Immobilization of glucose oxidase on reagentless ferrocene-containing polythiophene derivative and its glucose sensing application, Journal of Electroanalytical Chemistry, 639, 21–26
• Abu-Rabeah, K., Niţǎ, I.I., Tencaliec, A.M., Marks, R.S. (2009). New approach of constructing biosensing matrices by physical and chemical crosslinking of biotin-alginate with alginate-pyrrole, Electrochimica Acta, 54, 4359-4364
• Arslan F., Ustabaş S., Arslan H. (2011). An Amperometric Biosensor for Glucose Determination Prepared from Glucose Oxidase Immobilized in Polyaniline-Polyvinylsulfonate Film. Sensors, 11, 8152-8163
• Borole D. D., Kapadi U. R., Mahulikar P. P., Hundiwale, D. G. (2007). Glucose oxidase electrodes of polyaniline, poly(o-anisidine) their co-polymer as a biosensor: a comparative study, Journal of Material Science, 42,4947–4953
• Deng, H., Teo, A.K.L., Gao, Z. (2014). An interference-free glucose biosensor based on a novel low potentialredox polymer mediator. Sensors and Actuators B, 191, 522– 528
• Eftekhari, A. (2004). Electropolymerization of aniline onto passivated substrate and its application for preparation of enzyme-modified electrode, Synthetic Metals, 145, 211-216
• Ekiz, F., Oğuzkaya F., Akin M., Tanyeli C., Toppare L. (2011). Synthesis and application of poly-SNS-anchored carboxylic acid: a novel functional matrix for biomolecule conjugation, Journal of Materials Chemistry, 21, 12337-12343
• Guadalupe de Jesus, C., Lima, D., Santos, V., Wohnrath, K., Pessoa, C.A. (2013), Glucose biosensor based on the highly efficient immobilization of glucose oxidase on layer-by-layer films of silsesquioxane polyelectrolyte, Sensors and Actuators B, 186, 44-51
• Haighi, B., Nazari, L., Sajjadi, S.M., (2012). Fabrication and application of a sensitive and highly stable copper hexacyanoferrate modified carbon ionic liquid paste electrode for hydrogen peroxide and glucose detection, Electroanalysis, 24, 2165-2175.
• Huang, Y., Qin X., Li Z., Fu, Y., Qin C., Wu, F., Su, Z., Ma, M., Xie, Q., Yao, S.H., Hu, J. (2012). Fabrication of a chitosan/glucose oxidase–poly(anilineboronic acid)–Aunano/Au-plated Au electrode for biosensor and biofuel cell. Biosensors and Bioelectronics, 31, 357-362
• Jiang, Y., Zhang, Q., Li, F., Niu, L. (2012). Glucose oxidase and graphene bionanocomposite bridged by ionic liquid unit for glucose biosensing application, Sensors and Actuators B, 161, 728-733
• Kadam, S.B., Datta K., Ghosh P. & Shirsat, M.D (2011). Poly(Pyrrole)-Poly(N-Methylpyrrole) Composite Matrix for Amperometric Biosensor Design, International Journal of Polymeric Materials, 60,233–243
• Li, J., Yuan, R., Chai, Y., Che, X., Li, W. (2012). Construction of an amperometric glucose biosensor based on the immobilization of glucose oxidase onto electrodeposited Pt nanoparticles-chitosan composite film, Bioprocess Biosystem Engineering, 35, 1089-1095
• Olea, D., Viratelle, O., Faure, C. (2008), Polypyrrole-glucose oxidase biosensor. Effect of enzyme encapsulation in multilamellar vesicles on analytical properties, Biosensors and Bioelectronics, 23, 788-794
• Ozdemir, C., Yeni, F., Odaci, D., Timur, S. (2010) Electrochemical glucose biosensing by pyranose oxidase immobilized in gold nanoparticle-polyaniline/AgCl/gelatin nanocomposite matrix, Food Chemistry, 119, 380-385
• Ozyilmaz G., Ozyilmaz A.T., Can F. (2011). Glucose Oxidase- Polypyrrole Electrodes Synthesized In P-Toluenesulfonic Acid And Sodium p-Toluenesulfonate, Applied Biochemistry and Microbiology, 47,217-225
• Özyılmaz A.T., Erbil, M., Yazıcı, B. (2006). The electrochemical synthesis of polyaniline on stainless steel and its corrosion performance, Current Applied Physics, 6, 1-9
• Pauliukaite, R., Ghica, M.E., Fatibello-Filho, O., Brett, C.M.A. (2010). Electrochemical impedance studies of chitosan-modified electrodes for application in electrochemical sensors and biosensors. Electrochimica Acta, 55, 6239-6247
• Raicopol, M., Prunǎ, A., Damian, C., Pilan, L. (2013). Functionalized single-walled carbon nanotubes/polypyrrole composites for amperometric glucose biosensors, Nanoscale Research Letters, 8, 1-8
• Rothwell, S.A., Killoran S.J.,O’Neill, R.D. (2010). Enzyme Immobilization Strategies and Electropolymerization Conditions to Control Sensitivity and Selectivity Parameters of a Polymer-Enzyme Composite Glucose Biosensor, Sensors, 10, 6439-6462
• Salimi, A. Noorbakhsh, A (2011). Layer by layer assembly of glucose oxidase and thiourea onto glassy carbon electrode: fabrication of glucose biosensor, Electrochimica Acta, 56, 6097-6105
• Savale P. A. & Shirsat M. D., (2009). Synthesis of Poly(o-anisidine)/H2SO4 Film for the Development of Glucose Biosensor, Applied Biochemistry and Biotechnology, 159,299–309
• Shervedani, R.K., Hatefi-Mehrjardi, A. (2007). Electrochemical characterization of directly immobilized glucose oxidase on gold mercaptosuccinic anhydride self-assembled monolayer, Sensors and Actuators, B: Chemical, 126, 415-423
• Shkotova L.V., Piechniakova N.Y., Kukla O.L., Dzyadevych S.V. (2016). Thin-film amperometric multisensor for simulraneous determination of lactate and glucose in wine, Food Chemistry, 197, 972-978
• Tang, W., Li L., Zeng X., (2015). A glucose biosensor based on the synergistic action of nanometer-sized TiO2 and polyaniline, Talanta, 131, 417–423
• Uang, Y.M., Chou, T.S., 2003. Fabrication of glucose oxidase/polypyrrole biosensor by galvanostatic method in various pH aqueous solutions. Biosensors and Bioelectronics,19: 141-147
• Yao, Y., Wu, S.G., Xu, H.H., Wang, L.W. (2015). High-Sensitive Glucose Biosensor Based on Ionic Liquid DopedPolyaniline/Prussian Blue Composite Film 2015 Chınese Journal of Chemıcal Physıcs, 28, 755-761