Using Response Surface Methodology for Amperometric Glucose Biosensor Construction

Year 2018, Volume: 3 Issue: 1, 1 - 15, 04.01.2018

Gül Özyılmaz , Ali Tuncay Özyılmaz Seda Ağçam

https://doi.org/10.28978/nesciences.379311

Cited By: 5

Abstract

In this study, construction of amperometric glucose biosensor was carried out by immobilizing of
glucose oxidase (GOD) on platinum electrode with 0.09 cm2 surface area which coated with
polypyrrole (PPy) by cyclic voltammetry technique. Because measured current values in the
presence of glucose would be affected from the electrode preparing and working conditions,
experimental parameters should be optimized by response surface methodology (RSM). To this,
State Ease Design Expert 8.0.7.1. (Serial Number:0021-6578) programe was used. PPy synthesis
conditions of pyrrole (Py) monomer concentration and scan rate were optimized according to
current response in presence of glucose. Optimal Py monomer concentration and scan rate for PPy
synthesis were determined as 10 mM and 50 mV/s, respectively. Immobilization parameters such
as concentrations of chitosan, GOD and glutaraldehyde (GAL) also were optimized by RSM as 1.0
%, 4 mg/ml and 0.0625 %, respectively. The digital photos of electrodes at each stage were
obtained. All electrodes well characterized in absence and in the presence of glucose by cyclic
voltammetry and impedance techniques and it was observed that electrodes were sensitive to
glucose molecule. Finally the effect of working pH and applied potential on the current response
was investigated by RSM. The highest current response was obsreved when pH of glucose solution
and applied potential were 6.0 and 0.8, respectively.

Keywords

Response surface methodology, Pt electrode, Polypyrrole, amperometric biosensor, glucose oxidase

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