Karabiber özütü kullanılarak sentezlenen biyojenik gümüş nanopartiküller ile grafen yapıların kombine antibakteriyel ve antioksidan etkisi

Year 2025, Volume: 7 Issue: 1, 77 - 91

Selim Işıldak , Mahfuz Elmastas , Behiç Selman Erdoğdu , Meryem Erdoğdu

Abstract

Nanoteknoloji, malzemelerin nanometre ölçeğinde, özellikle 1 ila 100 nanometre aralığında tasarım ve manipülasyonuna odaklanan öncü bir araştırma alanıdır. Gümüş nanoparçacıkları, antiseptik özellikleri ile bilinir ve genellikle malzemelerin antibakteriyel etkinliğini artırmak amacı ile diş hekimliği gibi alanlarda kullanılır. Son zamanlarda, grafen bazlı malzemeler de antibakteriyel özellikleri nedeniyle önem kazanmıştır. Bu çalışma ile karabiber ekstraktı kullanılarak mikrodalga yöntemi ile sentezlenmiş gümüş nanoparçacıkları ile grafen yapılarının kombinasyonunun antibakteriyel ve antioksidan aktiviteleri incelenmiştir. Sentezlenen malzemeler, UV-Vis spektroskopisi, taramalı elektron mikroskobu (SEM) ve enerji dağıtıcı X-ışını spektroskopisi (EDS) gibi çeşitli analitik yöntemlerle karakterize edilmiştir. Grafen ve gümüş nanoparçacık kompozitlerinin antibakteriyel etkinliği, Staphylococcus aureus (S. aureus) üzerine agar well difüzyon yöntemi kullanılarak değerlendirilmiştir. Ayrıca, kompozitlerin serbest radikal süpürme aktiviteleri DPPH, FRAP ve ABTS testleri ile test edilmiştir. Sonuçlar, grafen oksit ve gümüş nanoparçacıklarının kombinasyonunun 14.40 mm ile en büyük inhibisyon alanını oluşturduğunu, antioksidan aktivitenin ise genel olarak gümüş nanoparçacıklar için grafen ve kompozit yapılara kıyasla daha üstün olduğunu göstermiştir. Bu sonuçlar, sentezlenen grafenin, biyosentezlenmiş gümüş nanoparçacıkları ile entegrasyonunun antibakteriyel aktiviteyi geliştirebileceği ve biyomedikal alanlarda kullanılabileceğini göstermektedir.

Keywords

Antibakteriyel, Antioksidan, Grafen Oksit, Gümüş nanopartikül

Combined Antibacterial and Antioxidant Effect of Graphene Structures with Biogenic Silver Nanoparticles Synthesized by Using Black Pepper Extract

Abstract

Nanotechnology is a pioneering research field that focuses on the design and manipulation of materials at the nanoscale, particularly within the range of 1 to 100 nanometers. Silver nanoparticles are known for their antiseptic properties and are commonly used in fields such as dentistry to enhance the antibacterial efficacy of materials Recently, graphene-based materials have also gained importance due to their antibacterial properties. In this study, antibacterial and antioxidant activities of the combination of silver nanoparticles and graphene structures synthesized by microwave method using black pepper extract were investigated. The synthesized materials were characterized by various analytical methods such as UV-Vis spectroscopy, scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The antibacterial activity of graphene and silver nanoparticle composites was evaluated using agar well diffusion method on Staphylococcus aureus (S. aureus). In addition, the free radical scavenging activities of the composites were tested by DPPH, FRAP and ABTS assays. The results showed that the combination of graphene oxide and silver nanoparticles produced the largest inhibition area of 14.40 mm, while the antioxidant activity was generally superior for silver nanoparticles compared to graphene and composite structures. These results suggest that the integration of synthesized graphene with biosynthesized silver nanoparticles can improve antibacterial activity and can be used in biomedical fields.

Keywords

Antibacterial, Antioxidant, Graphene Oxide, Silver nanoparticle

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