Productivity of Rotifer Brachionus Plicatilis Cultured Under Batch System by Using Sugar Beet Molasses as A Substitute Medium

Year 2019, Volume: 14 Issue: 4, 43 - 49, 26.10.2019

Servet Atayeter

Abstract

     This
study deals with the determination of availability of sugar beet molasses as a
nutritional enrichment factor for rotifer cultures. A series of experiments
under varying conditions such as ratio of sugar beet molasses in the nutrient
complex were carried out in order to determine the effectiveness of sugar beet
molasses as a carbohydrate and mineral source for the purpose of aquacultural
operations. The culture water of Brachionus plicatilis with the salinity of 38% was treated
through mechanical filtration and UV sterilization.  1 T cylindro-conical tanks are utilized for
rotifer culture. Parameters of culture water were stabilized at values as
following; Oxygen concentration:5 to 10 ppm, temperature: 27⁰C and pH:6.8 to 7.4.
Rotifers were counted by using Sedgewick Rafter Cell on a daily basis in order
to determine increase of number of individuals. The study was conducted as 3
different trial groups, each consisting three separate tanks, so triplicate
determinations were obtained. For the Group
I, 10% molasses by weight of Culture Selco Plus (200 gr Selco Plus+20 gr
molasses per tank) was added 6 times a day with 4 hours’ intervals. For the
Group II, 20% molasses by weight of Culture Selco Plus (200gr Selco Plus+40gr
molasses per tank) was added and lastly for the control group, Group III, no molasses has been added
and 200 gr Culture Selco Plus was only added per tank. The addition of molasses
to nutritive complex has a decreasing effect on the number of rotifer and
increasing effect on the number of ciliate. From the study, it is clear that
sugar beet molasses should not be used as an additional nutritional factor for
rotifer cultures due to its decreasing effect on the number rotifers and
stimulating effect on the ciliate booming. Addition of 10% and 20% molasses
resulted in 31.4% and 23.3% decrease in number of rotifers at the time of T3,
respectively. On the other hand, it was
observed that addition of molasses in the nutritive complex has not affected
on the percentage of rotifers bearing eggs.

Keywords

Sugar Beet Molasses, Ciliate, Brachionus Plicatilis, Rotifer, Culture Medium

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